##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l03n01_7331.34100000035c1f.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19808495 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.7349478594916 33.0 33.0 33.0 33.0 33.0 2 32.74697350808327 33.0 33.0 33.0 33.0 33.0 3 32.77684957893066 33.0 33.0 33.0 33.0 33.0 4 36.46552234281302 37.0 37.0 37.0 37.0 37.0 5 36.57532346601799 37.0 37.0 37.0 37.0 37.0 6 36.61202226620448 37.0 37.0 37.0 37.0 37.0 7 36.59995920942 37.0 37.0 37.0 37.0 37.0 8 36.608767854397826 37.0 37.0 37.0 37.0 37.0 9 36.60007946085758 37.0 37.0 37.0 37.0 37.0 10-11 36.58578720897272 37.0 37.0 37.0 37.0 37.0 12-13 36.552699662442805 37.0 37.0 37.0 37.0 37.0 14-15 38.63102413888586 40.0 37.0 40.0 37.0 40.0 16-17 38.59500479466007 40.0 37.0 40.0 37.0 40.0 18-19 38.57185927047966 40.0 37.0 40.0 37.0 40.0 20-21 38.51783325790274 40.0 37.0 40.0 37.0 40.0 22-23 38.453865046284434 40.0 37.0 40.0 37.0 40.0 24-25 38.40702552112111 40.0 37.0 40.0 37.0 40.0 26-27 38.31979572400629 40.0 37.0 40.0 37.0 40.0 28-29 38.26217289602264 40.0 37.0 40.0 37.0 40.0 30-31 38.204444406301434 40.0 37.0 40.0 37.0 40.0 32-33 38.10450435532836 40.0 37.0 40.0 37.0 40.0 34-35 38.03931164381746 40.0 37.0 40.0 37.0 40.0 36-37 37.937743755898666 40.0 37.0 40.0 35.0 40.0 38-39 37.817829345439925 40.0 37.0 40.0 33.0 40.0 40-41 37.69136373056105 40.0 37.0 40.0 33.0 40.0 42-43 37.558527364143515 40.0 37.0 40.0 33.0 40.0 44-45 37.402216271352266 40.0 37.0 40.0 33.0 40.0 46-47 37.21220433455444 37.0 37.0 40.0 33.0 40.0 48-49 36.98315457585243 37.0 37.0 40.0 33.0 40.0 50-51 36.76461460095783 37.0 37.0 40.0 33.0 40.0 52-53 36.51911250703297 37.0 37.0 40.0 33.0 40.0 54-55 36.25915492317816 37.0 37.0 40.0 33.0 40.0 56-57 35.97737389943052 37.0 37.0 40.0 33.0 40.0 58-59 35.69360072534536 37.0 33.0 37.0 33.0 40.0 60-61 35.40024120964263 37.0 33.0 37.0 33.0 40.0 62-63 35.142994811064646 37.0 33.0 37.0 33.0 40.0 64-65 34.86857954125237 37.0 33.0 37.0 27.0 40.0 66-67 34.63062812192446 37.0 33.0 37.0 27.0 37.0 68-69 34.36200127773463 37.0 33.0 37.0 27.0 37.0 70-71 34.12544865220704 37.0 33.0 37.0 27.0 37.0 72-73 33.90936605734055 37.0 33.0 37.0 27.0 37.0 74-75 33.67191520607699 37.0 33.0 37.0 27.0 37.0 76-77 30.759664073419003 33.0 30.0 33.0 24.5 35.0 78-79 33.45989902312114 35.0 33.0 37.0 27.0 37.0 80-81 33.97099289976346 37.0 33.0 37.0 27.0 37.0 82-83 33.96291929295991 37.0 33.0 37.0 27.0 37.0 84-85 33.86774813028451 37.0 33.0 37.0 27.0 37.0 86-87 33.77563658925123 37.0 33.0 37.0 27.0 37.0 88-89 33.62464700119823 37.0 33.0 37.0 27.0 37.0 90-91 33.5094632378684 37.0 33.0 37.0 27.0 37.0 92-93 33.388299666380505 37.0 33.0 37.0 27.0 37.0 94-95 33.272786397957034 37.0 33.0 37.0 27.0 37.0 96-97 33.17978889865182 33.0 33.0 37.0 27.0 37.0 98-99 33.02907028020049 33.0 33.0 37.0 27.0 37.0 100-101 31.007250348903337 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.08899262424954202 2306 2 0.09893762801641515 2306 3 0.09752186552689324 2306 4 -0.1526452223611372 2306 5 -0.07278873039746259 2306 6 0.04854965688112145 2306 7 0.08046260733534893 2306 8 0.057526148227090346 2306 9 0.05807946511968254 2306 10-11 0.10987603677941138 2306 12-13 0.11573487041957975 2306 14-15 0.1863110731501152 2306 16-17 0.18729900760789775 2306 18-19 0.15061304707907652 2306 20-21 0.14172762006068496 2306 22-23 0.1386718349404461 2306 24-25 0.1431662443374151 2306 26-27 0.16152247828094346 2306 28-29 0.09185432218859546 2306 30-31 0.09060637775704805 2306 32-33 0.12244159760354734 2306 34-35 0.09584612783472579 2306 36-37 0.1059160808567512 2306 38-39 0.01827764305780022 2306 40-41 -0.01812792358898463 2306 42-43 0.06742817837848492 2306 44-45 0.02156686202116731 2306 46-47 -0.04296742946083043 2306 48-49 -0.0033527213993878036 2306 50-51 -0.047498811903963656 2306 52-53 -0.0782809340921986 2306 54-55 -0.06031377516888625 2306 56-57 -0.14937880034553075 2306 58-59 -0.2284796138328673 2306 60-61 -0.15037132792068064 2306 62-63 -0.14396921152187048 2306 64-65 -0.1824019787753457 2306 66-67 -0.24332011675375043 2306 68-69 -0.23747515686739717 2306 70-71 -0.3121154136116999 2306 72-73 -0.4138918975229444 2306 74-75 -0.4053011046826285 2306 76-77 -0.6684188920798739 2306 78-79 -0.2682148726613889 2306 80-81 -0.14662294699624567 2306 82-83 -0.17505159674820447 2306 84-85 -0.26622231807368735 2306 86-87 -0.3582348823450161 2306 88-89 -0.3773000957141619 2306 90-91 -0.4676184055372161 2306 92-93 -0.47236047343704257 2306 94-95 -0.5047583864494385 2306 96-97 -0.5911679378428474 2306 98-99 -0.6561699054604233 2306 100-101 -0.9925344373124076 2307 1 0.09532125597635144 2307 2 0.07291985033990045 2307 3 0.06505164981076206 2307 4 -0.03437878617439338 2307 5 0.006004496874489007 2307 6 0.08858107093121959 2307 7 0.11904490227195907 2307 8 0.1327662089975945 2307 9 0.08642017849481931 2307 10-11 0.06887050549055118 2307 12-13 0.09592197894007626 2307 14-15 0.15502233591927705 2307 16-17 0.11422067296240357 2307 18-19 0.06645606630119971 2307 20-21 0.09653281067986796 2307 22-23 0.09270726949157648 2307 24-25 0.08995162879465823 2307 26-27 0.01019257549452135 2307 28-29 -0.03296200891474399 2307 30-31 0.061545963124522984 2307 32-33 0.006915229220773256 2307 34-35 0.05928551062109477 2307 36-37 0.01646410952646704 2307 38-39 0.005914708316417716 2307 40-41 -0.03722323253322912 2307 42-43 -0.12031004544255097 2307 44-45 -0.040495845450784884 2307 46-47 -0.08875227256719853 2307 48-49 -0.11857293009074255 2307 50-51 -0.18047708156025521 2307 52-53 -0.12431171394971585 2307 54-55 -0.09667002252850665 2307 56-57 -0.16389753920986294 2307 58-59 -0.23729694955783742 2307 60-61 -0.2794906534487538 2307 62-63 -0.29187956623450617 2307 64-65 -0.32126852460075384 2307 66-67 -0.3166916512841098 2307 68-69 -0.413289668335743 2307 70-71 -0.4740850006404571 2307 72-73 -0.5534535926104098 2307 74-75 -0.5104695050602572 2307 76-77 -0.7726084601936485 2307 78-79 -0.3380075381593741 2307 80-81 -0.19430761959411313 2307 82-83 -0.20640377407733013 2307 84-85 -0.32901900212765156 2307 86-87 -0.41027780546745873 2307 88-89 -0.39301223087585413 2307 90-91 -0.46046578627044 2307 92-93 -0.4244220317627381 2307 94-95 -0.5897986442541594 2307 96-97 -0.6457983807749059 2307 98-99 -0.7577747063667104 2307 100-101 -1.079028590038721 2201 1 0.051039528928320976 2201 2 0.057558339367759004 2201 3 0.048935407680687604 2201 4 -0.11314516049782952 2201 5 -0.052444276402717094 2201 6 0.02592239385212025 2201 7 0.09586796028427358 2201 8 0.08020650012937836 2201 9 0.09129595147290814 2201 10-11 0.07301530635945852 2201 12-13 0.07636053789513397 2201 14-15 0.13805272615354625 2201 16-17 0.023603916633867073 2201 18-19 0.08952663794496374 2201 20-21 0.016267434783863166 2201 22-23 0.04422826478698738 2201 24-25 0.0020330942138215846 2201 26-27 0.04883156267754174 2201 28-29 0.0527820212932113 2201 30-31 0.05080658531101534 2201 32-33 0.049384782420411 2201 34-35 -0.006870592616060378 2201 36-37 0.04182735491318823 2201 38-39 0.04335641331888951 2201 40-41 0.07084905098560768 2201 42-43 -0.019307894159901196 2201 44-45 -0.08794675711482824 2201 46-47 -0.05575892519744485 2201 48-49 -0.09095662010872019 2201 50-51 -0.11092177608940545 2201 52-53 -0.04984019118409577 2201 54-55 0.005807141858035436 2201 56-57 -0.16940144581248973 2201 58-59 -0.15275562308535484 2201 60-61 -0.18847862672534887 2201 62-63 -0.23275583553240864 2201 64-65 -0.2361045263077628 2201 66-67 -0.32273232804742946 2201 68-69 -0.45137085318642534 2201 70-71 -0.4405442553328953 2201 72-73 -0.4443455365211264 2201 74-75 -0.5722589583674065 2201 76-77 -1.0275613021646848 2201 78-79 -0.42376144952346806 2201 80-81 -0.22913399387536515 2201 82-83 -0.18451925712531647 2201 84-85 -0.258233341783793 2201 86-87 -0.3631733742289782 2201 88-89 -0.3371538841839765 2201 90-91 -0.31634754828366596 2201 92-93 -0.35572697048774415 2201 94-95 -0.5403643289719184 2201 96-97 -0.4361956462376 2201 98-99 -0.5545107817891619 2201 100-101 -0.8621131418883259 1101 1 0.039285398112852477 1101 2 0.05550837183950108 1101 3 0.0685416296311061 1101 4 -0.11622352018289206 1101 5 -0.08189883106280149 1101 6 0.018397020436069056 1101 7 0.01794612889074898 1101 8 0.060197584966161344 1101 9 0.047598796645637265 1101 10-11 0.04695104077666201 1101 12-13 0.040108264404089766 1101 14-15 0.060602025890140965 1101 16-17 0.06761925888581999 1101 18-19 0.05203383617227786 1101 20-21 0.007491035981736616 1101 22-23 0.04747831433719085 1101 24-25 0.03087022506030479 1101 26-27 0.056147072230537276 1101 28-29 0.008907244507547318 1101 30-31 0.02511018587296121 1101 32-33 -0.0781812474209218 1101 34-35 -0.057576050928581424 1101 36-37 -0.10290301634339016 1101 38-39 -0.08254034596794924 1101 40-41 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2304 2 0.07194502253592816 2304 3 0.06426747263517285 2304 4 -0.11094886247841629 2304 5 -0.07060831929667444 2304 6 0.008715304934973744 2304 7 0.07342804129089586 2304 8 0.03402858431459066 2304 9 0.030850422639289832 2304 10-11 0.030862278888768913 2304 12-13 0.026092934835183712 2304 14-15 0.10058892376869721 2304 16-17 0.06873513228901373 2304 18-19 0.0362765683884021 2304 20-21 0.03712391145485583 2304 22-23 0.05273480316763113 2304 24-25 0.05530649595355186 2304 26-27 0.07353084007991839 2304 28-29 -0.03783579431263462 2304 30-31 0.023177805199537715 2304 32-33 0.04511775806300733 2304 34-35 0.032039057494053225 2304 36-37 -0.04847916697949728 2304 38-39 -0.024312256830803847 2304 40-41 -0.010947561030441477 2304 42-43 -0.027890290306181953 2304 44-45 -0.02735660451738653 2304 46-47 -0.08948270292676597 2304 48-49 -0.05427565763128683 2304 50-51 -0.11513954262201764 2304 52-53 -0.04906441084947488 2304 54-55 -0.1670052671674611 2304 56-57 -0.15912581283280502 2304 58-59 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14-15 0.036400269988256184 2202 16-17 -0.015294418356596395 2202 18-19 -0.01497824480102139 2202 20-21 -0.046320718169347685 2202 22-23 0.02478094001940434 2202 24-25 -0.05981298920391254 2202 26-27 -0.07942528600904808 2202 28-29 -0.014372609565548089 2202 30-31 0.010376535686425825 2202 32-33 -0.012171928796966824 2202 34-35 -0.02914326312819071 2202 36-37 -0.035205891672383416 2202 38-39 -0.0978626604777233 2202 40-41 -0.11806895192452771 2202 42-43 -0.10667678231587985 2202 44-45 -0.12204219362539703 2202 46-47 -0.13753132156666936 2202 48-49 -0.09916995110512516 2202 50-51 -0.11123270612888092 2202 52-53 -0.1361033113747041 2202 54-55 -0.12127239228284736 2202 56-57 -0.21478218724318765 2202 58-59 -0.2090121603820947 2202 60-61 -0.09286294979282417 2202 62-63 -0.18194309946598963 2202 64-65 -0.21840756635444336 2202 66-67 -0.23342752484920481 2202 68-69 -0.2433534864221727 2202 70-71 -0.2911345682948152 2202 72-73 -0.33085158456934494 2202 74-75 -0.3864680677335386 2202 76-77 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1213 48-49 -0.2721178683099197 1213 50-51 -0.21497313855473266 1213 52-53 -0.2377985051635889 1213 54-55 -0.24053154694466627 1213 56-57 -0.22836354267580816 1213 58-59 -0.18470531747654206 1213 60-61 -0.22944711278352514 1213 62-63 -0.17387835207586733 1213 64-65 -0.14060534437700767 1213 66-67 -0.09838405323133514 1213 68-69 -0.08008966431004438 1213 70-71 -0.015104984948990818 1213 72-73 0.08842691248540291 1213 74-75 0.051868271463128224 1213 76-77 0.3329461929245916 1213 78-79 -0.2482741428387243 1213 80-81 -0.21486728778974395 1213 82-83 -0.03883832168936863 1213 84-85 -0.09949817171731468 1213 86-87 -0.05062876366003621 1213 88-89 -0.14392444581078223 1213 90-91 -0.05610869499762572 1213 92-93 -0.014469847036487238 1213 94-95 0.10534297756002786 1213 96-97 0.13287379212346906 1213 98-99 0.18353709212501457 1213 100-101 0.8496541043089678 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 46.0 8 520.0 9 1543.0 10 989.0 11 389.0 12 578.0 13 726.0 14 1412.0 15 2908.0 16 5139.0 17 7758.0 18 11504.0 19 15796.0 20 21421.0 21 28388.0 22 37141.0 23 48417.0 24 63591.0 25 82726.0 26 107030.0 27 139140.0 28 179730.0 29 233109.0 30 304268.0 31 406453.0 32 558739.0 33 814291.0 34 1318469.0 35 2500961.0 36 5290896.0 37 6617752.0 38 1006584.0 39 80.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 21.029749494671567 22.396528149926056 30.478123864233503 26.095598491168875 2 27.9509675015694 22.672136373813355 29.013673174059917 20.363222950557326 3 21.955474153892055 20.7401925285086 35.98485902134412 21.319474296255216 4 21.881576190403905 21.090091558771597 34.969831486552785 22.058500764271717 5 28.043286478856672 20.287603879042805 31.056998525127728 20.612111116972795 6 29.78573081902487 20.915349702236337 29.342840028987567 19.95607944975123 7 28.18231773792002 26.683445663085458 24.571942492349873 20.56229410664465 8 26.727573195237696 22.454270251223026 29.329638622217384 21.48851793132189 9 23.305930107259538 21.441856132936906 32.94602139132731 22.306192368476253 10-11 27.54658265703865 22.199116943753737 27.407401555034994 22.84689884417262 12-13 26.710305351315185 25.414790472471534 24.4315860442704 23.443318131942885 14-15 26.307705355707235 23.824604039832405 25.75699971148742 24.11069089297294 16-17 25.857576159836725 23.56534169681851 25.283117891604906 25.293964251739855 18-19 25.832979739248234 24.05719616760385 25.6492075748309 24.460616518317018 20-21 24.972045632674387 24.41720148373083 26.09954475866656 24.511208124928217 22-23 25.089910102266227 24.570991282131512 25.50677624241881 24.832322373183455 24-25 25.565326896364414 24.42336229986175 25.215015073078494 24.79629573069534 26-27 25.20893330803776 24.536463535757473 25.54451948191466 24.710083674290104 28-29 25.216531853503376 25.054817647582073 24.957230270266123 24.77142022864843 30-31 25.488406145592744 24.809348023932408 24.820275454033585 24.88197037644126 32-33 25.248374546359862 25.096349425702368 24.973428355407354 24.681847672530413 34-35 25.05297594311996 25.232542234161787 24.928982362353608 24.785499460364647 36-37 25.25970844455996 24.97055266478031 24.975558478264006 24.79418041239573 38-39 25.253953306228755 24.688120897259353 25.093917041574944 24.96400875493695 40-41 25.02907520757574 25.05181835763759 24.753039658989575 25.1660667757971 42-43 25.02181288576773 24.97210704764412 24.862900620156694 25.143179446431457 44-45 25.366515625330145 24.743702670838864 24.85299578107642 25.03678592275457 46-47 24.957079157558123 24.860971309551665 25.077056813095673 25.104892719794535 48-49 25.10588879917732 24.625364426261118 24.936395023045932 25.33235175151563 50-51 24.963866939656583 24.92909397337336 25.357156913401656 24.7498821735684 52-53 25.11687231424173 24.70428541328192 25.438922208206233 24.739920064270116 54-55 24.86367027523862 24.876412114244737 24.978465484196064 25.281452126320573 56-57 25.004101531758632 25.087505656175146 25.177999408894664 24.730393403171558 58-59 24.80679179054042 25.267109525800898 25.147692795752853 24.778405887905834 60-61 25.35188236740275 25.104768258129294 24.673592233847977 24.869757140619974 62-63 24.940368226218876 25.282726010096194 25.160964578540828 24.615941185144095 64-65 25.161638819279926 25.16331517375225 24.96089789584946 24.714148111118366 66-67 25.29854333529955 25.246914092995603 25.0620471190244 24.392495452680446 68-69 25.17975558905895 24.917748211634958 25.40686803884699 24.4956281604591 70-71 25.026039586230898 25.382971034728776 25.041244704846477 24.54974467419385 72-73 24.937161226943726 24.994273955916366 25.352984010683127 24.71558080645678 74-75 24.99550013286885 24.93759330012769 25.27412958279181 24.792776984211645 76-77 24.86804466122428 25.000521412900184 25.15207707921916 24.97935684665638 78-79 25.08317029491524 24.869132510395783 24.99735927244775 25.050337922241226 80-81 24.980609467789908 24.757374119815236 25.32325757710005 24.938758835294802 82-83 24.737056783860005 25.516398549733545 25.140732927503894 24.60581173890256 84-85 24.92075297360957 25.20707279174067 25.0133435325333 24.85883070211646 86-87 24.82317770257738 25.299504499138532 25.15615601861374 24.72116177967035 88-89 24.881066958600414 26.277849762517686 24.487138184106104 24.353945094775792 90-91 24.91060850153444 25.373716900867127 24.73059201340584 24.985082584192593 92-93 24.66725129852428 25.706931689900575 24.70559835458105 24.92021865699409 94-95 24.755627246484124 25.88518358475253 24.36140429455888 24.997784874204466 96-97 24.659200291961838 25.410278486677928 24.63076097502652 25.29976024633371 98-99 24.955436963155996 25.347268533571683 24.600192463341166 25.09710203993116 100-101 25.217462227913064 25.93096606130645 24.05724262081962 24.794329089960872 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 9022.0 1 5495.5 2 3056.5 3 3664.0 4 3184.0 5 2563.0 6 1815.5 7 1726.5 8 1750.0 9 1714.0 10 1571.5 11 1444.5 12 1401.0 13 1360.5 14 1401.5 15 1464.0 16 1635.0 17 1774.0 18 1836.5 19 2137.0 20 2481.0 21 2834.0 22 3505.0 23 4528.5 24 5582.0 25 6705.5 26 8585.5 27 10671.5 28 12749.0 29 15317.0 30 18885.5 31 24716.0 32 33392.0 33 43965.5 34 58191.5 35 78604.0 36 103109.0 37 131161.5 38 164680.0 39 213994.0 40 285376.5 41 361570.0 42 428913.0 43 520387.0 44 639955.0 45 793528.5 46 982869.5 47 1175974.5 48 1323292.5 49 1361347.0 50 1308511.0 51 1234270.5 52 1160166.5 53 1105533.0 54 1048003.5 55 989189.5 56 956595.0 57 832206.5 58 635374.0 59 475349.0 60 359474.0 61 276220.0 62 198831.0 63 130574.5 64 82957.0 65 51007.0 66 30078.5 67 18453.5 68 13411.0 69 9985.5 70 7506.5 71 5728.5 72 4428.0 73 3245.5 74 2326.0 75 1691.5 76 1236.0 77 930.0 78 746.0 79 573.5 80 386.0 81 261.5 82 183.0 83 139.0 84 108.5 85 76.5 86 56.0 87 52.0 88 51.5 89 41.5 90 32.5 91 27.5 92 23.0 93 19.0 94 15.0 95 13.0 96 11.5 97 7.5 98 6.0 99 4.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11534445196366508 2 0.0 3 0.0 4 0.5652423366843367 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.588082537315429E-4 12-13 0.0 14-15 0.0 16-17 3.28142042088508E-5 18-19 0.0 20-21 0.0016154685148972701 22-23 4.947372326872889E-4 24-25 0.0 26-27 1.0601512129013335E-4 28-29 4.6949553714201907E-4 30-31 0.0027462964753253594 32-33 0.0012898506423632892 34-35 0.0010677237215649144 36-37 0.0076961929717527765 38-39 0.015064243901417043 40-41 0.012779870454570123 42-43 0.009937655536172738 44-45 0.008408008786129385 46-47 0.01585430897198399 48-49 0.015387337604396496 50-51 0.014536692464520905 52-53 0.016831162589585932 54-55 0.01848449364780111 56-57 0.024827731738327422 58-59 0.032369950367254055 60-61 0.017540454234408014 62-63 0.008736150828217893 64-65 0.01824722170967557 66-67 0.02420931019746831 68-69 0.03124921908504407 70-71 0.030052762716198275 72-73 0.0324582963016625 74-75 0.025910600477219495 76-77 0.032965654382122414 78-79 0.016896790998003636 80-81 0.005795493297193957 82-83 0.008339856208157158 84-85 0.015245984109342987 86-87 0.02879572627804384 88-89 0.024812586721000257 90-91 0.035214689455205964 92-93 0.02599642224207341 94-95 0.03602747205176365 96-97 0.01598808995837392 98-99 0.01860060544730935 100-101 0.28610957066652465 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.9808495E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.684137664458696 #Duplication Level Percentage of deduplicated Percentage of total 1 79.86345757600736 19.713605811658223 2 9.131924101840129 4.5082734334242005 3 3.04463565485953 2.2546261692801597 4 1.871915629099811 1.848264923398061 5 0.7819005203540296 0.9650270042165376 6 0.5653519963872635 0.8373135904619861 7 0.5048881417017661 0.8723909876443386 8 0.4065231734972314 0.802773918271863 9 0.2880982584563103 0.6400311365363728 >10 2.5488169827161347 12.953309325613851 >50 0.43509742599764367 7.540035009215365 >100 0.46827976186740267 24.18103602994081 >500 0.05849356052225642 9.959550822483296 >1k 0.030312596515997003 12.426819251274852 >5k 3.0462017683972995E-4 0.49694258658026386 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.0601512129013335E-4 0.0 0.0 2 1.1106346039918732E-4 0.0 0.0 3 1.2116013861729525E-4 0.0 0.0 4 1.2116013861729525E-4 0.0 0.0 5 1.312568168354032E-4 0.0 0.0 6 1.3630515594445717E-4 0.0 0.0 7 1.464018341625651E-4 0.0 0.0 8 1.6659519059878097E-4 0.0 0.0 9 1.766918688168889E-4 0.0 0.0 10-11 2.3474776857100956E-4 0.0 0.0 12-13 2.9532783787965716E-4 0.0 0.0 14-15 4.0639129827884453E-4 0.0 0.0 16-17 5.553173019959366E-4 0.0 0.0 18-19 7.143399839311366E-4 0.0 0.0 20-21 9.036527005206605E-4 0.0 0.0 22-23 0.0011888838601822097 0.0 0.0 24-25 0.001486735867616394 0.0 0.0 26-27 0.0017643945186143623 0.0 0.0 28-29 0.0020496256782759114 0.0 0.0 30-31 0.0024484444678911747 0.0 0.0 32-33 0.0029658992265692067 0.0 0.0 34-35 0.0035186923590106167 0.0 0.0 36-37 0.00434409580334094 0.0 0.0 38-39 0.00542444037267849 0.0 0.0 40-41 0.006699145997714617 0.0 0.0 42-43 0.008524120585637627 0.0 0.0 44-45 0.010752962302284955 0.0 0.0 46-47 0.01355983884691896 0.0 0.0 48-49 0.01714415961434728 0.0 0.0 50-51 0.021750769051359024 0.0 0.0 52-53 0.02730646624087292 0.0 0.0 54-55 0.034343850958894154 0.0 0.0 56-57 0.04251206363734347 0.0 0.0 58-59 0.05302270566239384 0.0 0.0 60-61 0.06636041758851442 0.0 0.0 62-63 0.08299217078329273 0.0 0.0 64-65 0.10324358311926271 0.0 0.0 66-67 0.12822024086130723 0.0 0.0 68-69 0.1591867529562443 5.048339109053969E-6 0.0 70-71 0.1973799624857921 5.048339109053969E-6 0.0 72-73 0.2444229104735115 5.048339109053969E-6 0.0 74-75 0.30134798226720405 5.048339109053969E-6 0.0 76-77 0.370421882126835 5.048339109053969E-6 0.0 78-79 0.45533999428023175 5.048339109053969E-6 0.0 80-81 0.5570135439365788 5.048339109053969E-6 0.0 82-83 0.6772574090055807 5.048339109053969E-6 0.0 84-85 0.8215894241334336 5.048339109053969E-6 0.0 86-87 0.992132415915495 5.048339109053969E-6 0.0 88-89 1.1929402006563345 5.048339109053969E-6 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 149355 0.0 69.420334 1 TTTGTGT 158325 0.0 65.527885 2 TTGTGTT 181890 0.0 58.776077 3 AACTTTG 17555 0.0 56.816372 2 TAACTTT 17470 0.0 56.528088 1 TGTGTTT 196040 0.0 54.8967 4 ACTTTGT 38990 0.0 54.43229 1 GTGTTTG 224860 0.0 47.981747 5 GTTTGAG 182710 0.0 34.512608 2 TGTTTGA 342790 0.0 34.384014 1 TTGAGTC 25260 0.0 34.079987 4 TTTGAGT 69320 0.0 33.681095 3 TTTGAGG 68035 0.0 32.877586 8 GTTTGAA 28910 0.0 32.386604 2 TTGAGGG 35720 0.0 31.071348 9 TTTGAGC 48880 0.0 29.271103 8 TTGAGGC 13915 0.0 26.814295 9 TTGATCT 9065 0.0 26.651382 4 GTTTGAC 73160 0.0 25.208347 7 TTGAGGT 21400 0.0 25.055498 4 >>END_MODULE