FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l03n01_7331.34100000035c1f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l03n01_7331.34100000035c1f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19808495
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1493550.069.4203341
TTTGTGT1583250.065.5278852
TTGTGTT1818900.058.7760773
AACTTTG175550.056.8163722
TAACTTT174700.056.5280881
TGTGTTT1960400.054.89674
ACTTTGT389900.054.432291
GTGTTTG2248600.047.9817475
GTTTGAG1827100.034.5126082
TGTTTGA3427900.034.3840141
TTGAGTC252600.034.0799874
TTTGAGT693200.033.6810953
TTTGAGG680350.032.8775868
GTTTGAA289100.032.3866042
TTGAGGG357200.031.0713489
TTTGAGC488800.029.2711038
TTGAGGC139150.026.8142959
TTGATCT90650.026.6513824
GTTTGAC731600.025.2083477
TTGAGGT214000.025.0554984