##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l03n01_6957.34100000035bda.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26458576 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.73669066695048 33.0 33.0 33.0 33.0 33.0 2 32.74825281602457 33.0 33.0 33.0 33.0 33.0 3 32.781501997688764 33.0 33.0 33.0 33.0 33.0 4 36.471261945465244 37.0 37.0 37.0 37.0 37.0 5 36.581419876867145 37.0 37.0 37.0 37.0 37.0 6 36.61603625229113 37.0 37.0 37.0 37.0 37.0 7 36.59781573278925 37.0 37.0 37.0 37.0 37.0 8 36.60891141685025 37.0 37.0 37.0 37.0 37.0 9 36.598091409001 37.0 37.0 37.0 37.0 37.0 10-11 36.588009479421714 37.0 37.0 37.0 37.0 37.0 12-13 36.546267833915174 37.0 37.0 37.0 37.0 37.0 14-15 38.60826104171291 40.0 37.0 40.0 37.0 40.0 16-17 38.57480805089435 40.0 37.0 40.0 37.0 40.0 18-19 38.54218641623041 40.0 37.0 40.0 37.0 40.0 20-21 38.4856682196351 40.0 37.0 40.0 37.0 40.0 22-23 38.42434383090004 40.0 37.0 40.0 37.0 40.0 24-25 38.37238812474261 40.0 37.0 40.0 37.0 40.0 26-27 38.277466463047745 40.0 37.0 40.0 37.0 40.0 28-29 38.204507396769955 40.0 37.0 40.0 37.0 40.0 30-31 38.15200164211407 40.0 37.0 40.0 37.0 40.0 32-33 38.04748887468472 40.0 37.0 40.0 37.0 40.0 34-35 37.9743355046772 40.0 37.0 40.0 37.0 40.0 36-37 37.85449793291974 40.0 37.0 40.0 33.0 40.0 38-39 37.73379846670509 40.0 37.0 40.0 33.0 40.0 40-41 37.59946432869252 40.0 37.0 40.0 33.0 40.0 42-43 37.45935346255974 40.0 37.0 40.0 33.0 40.0 44-45 37.318189667501386 40.0 37.0 40.0 33.0 40.0 46-47 37.147844918033385 38.5 37.0 40.0 33.0 40.0 48-49 36.93680899531403 37.0 37.0 40.0 33.0 40.0 50-51 36.73478650173766 37.0 37.0 40.0 33.0 40.0 52-53 36.51732035767911 37.0 37.0 40.0 33.0 40.0 54-55 36.27545201223225 37.0 37.0 40.0 33.0 40.0 56-57 36.00557934410378 37.0 37.0 40.0 33.0 40.0 58-59 35.72061423487039 37.0 35.0 40.0 33.0 40.0 60-61 35.437412353559765 37.0 33.0 37.0 33.0 40.0 62-63 35.18876108449676 37.0 33.0 37.0 30.0 40.0 64-65 34.90872624815485 37.0 33.0 37.0 27.0 40.0 66-67 34.66827349287429 37.0 33.0 37.0 27.0 40.0 68-69 34.385115170219294 37.0 33.0 37.0 27.0 37.0 70-71 34.13089275855208 37.0 33.0 37.0 27.0 37.0 72-73 33.89577464410783 37.0 33.0 37.0 27.0 37.0 74-75 33.64275792846902 37.0 33.0 37.0 27.0 37.0 76-77 30.750903733443554 33.0 30.0 33.0 24.5 37.0 78-79 33.383185701301535 35.0 33.0 37.0 27.0 37.0 80-81 33.85035385124279 37.0 33.0 37.0 27.0 37.0 82-83 33.82847648339049 37.0 33.0 37.0 27.0 37.0 84-85 33.72526716857325 37.0 33.0 37.0 27.0 37.0 86-87 33.61587210891471 37.0 33.0 37.0 27.0 37.0 88-89 33.46481736205305 37.0 33.0 37.0 27.0 37.0 90-91 33.34166851987801 37.0 33.0 37.0 27.0 37.0 92-93 33.21289656707148 37.0 33.0 37.0 27.0 37.0 94-95 33.09156974283121 35.0 33.0 37.0 27.0 37.0 96-97 32.98579817749829 33.0 33.0 37.0 27.0 37.0 98-99 32.826787106758886 33.0 33.0 37.0 27.0 37.0 100-101 30.836293759724633 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.08295344211258993 2306 2 0.10624646396600212 2306 3 0.09714167790106387 2306 4 -0.10043868381588794 2306 5 -0.03124192126846026 2306 6 0.058855902397233706 2306 7 0.1057266231248164 2306 8 0.058126844343377115 2306 9 0.06708625620212416 2306 10-11 0.08961356203812443 2306 12-13 0.12333969137020517 2306 14-15 0.18841113308118196 2306 16-17 0.16534204563620136 2306 18-19 0.13536725808312866 2306 20-21 0.15114588299613274 2306 22-23 0.13258322819076795 2306 24-25 0.10028303247558057 2306 26-27 0.12874250404606613 2306 28-29 0.10124374805705827 2306 30-31 0.10661786629074754 2306 32-33 0.12865384216745923 2306 34-35 0.1221140239622045 2306 36-37 0.16586179233798504 2306 38-39 0.08564252964449537 2306 40-41 0.011786178249813872 2306 42-43 0.05935996506067198 2306 44-45 0.031250208940647894 2306 46-47 -0.025446808872274573 2306 48-49 -0.010780712851236274 2306 50-51 -0.014661727999509822 2306 52-53 -0.013725233905908851 2306 54-55 -0.03631514306975703 2306 56-57 -0.12078626928116165 2306 58-59 -0.19693759068685068 2306 60-61 -0.16035057901277838 2306 62-63 -0.13387670009359454 2306 64-65 -0.20160864380635246 2306 66-67 -0.22186407426212185 2306 68-69 -0.2979749638116047 2306 70-71 -0.2858896355981244 2306 72-73 -0.43648171515161494 2306 74-75 -0.46493059051588403 2306 76-77 -0.7033528509376019 2306 78-79 -0.2840144844071304 2306 80-81 -0.12489929905774488 2306 82-83 -0.11295925010661279 2306 84-85 -0.24871732804570712 2306 86-87 -0.3043709223412918 2306 88-89 -0.377725074953581 2306 90-91 -0.4130809068588519 2306 92-93 -0.42103495970606986 2306 94-95 -0.4959546313881944 2306 96-97 -0.5582746329854444 2306 98-99 -0.6143279475462649 2306 100-101 -1.0269634082825334 2307 1 0.0852175996291038 2307 2 0.0730490754343407 2307 3 0.08164896548921519 2307 4 -0.07816563432343315 2307 5 -0.021214905374506543 2307 6 0.07741978941844962 2307 7 0.09652065783667751 2307 8 0.11909663913478141 2307 9 0.05430225205237349 2307 10-11 0.07716763557099426 2307 12-13 0.11246511351983202 2307 14-15 0.17457794184087305 2307 16-17 0.10303099682150929 2307 18-19 0.10036188409577562 2307 20-21 0.11635469727240633 2307 22-23 0.10033592242224643 2307 24-25 0.0911985359996379 2307 26-27 0.0018229442337087676 2307 28-29 -0.03296541687684851 2307 30-31 0.029447043888687574 2307 32-33 -0.02805358239220368 2307 34-35 0.07028992915802945 2307 36-37 0.030122388258938315 2307 38-39 -0.014223670263511679 2307 40-41 0.017406179741819017 2307 42-43 -0.039576224500592616 2307 44-45 -0.03049342921816134 2307 46-47 -0.04591934495582706 2307 48-49 -0.07491151499468174 2307 50-51 -0.10497853077074382 2307 52-53 -0.10734314971179515 2307 54-55 -0.06905729895225932 2307 56-57 -0.11763103294404686 2307 58-59 -0.21284998706475733 2307 60-61 -0.31216253594543275 2307 62-63 -0.25702745320262466 2307 64-65 -0.2856290794939582 2307 66-67 -0.2968768866600513 2307 68-69 -0.31169970706211103 2307 70-71 -0.44425727327448783 2307 72-73 -0.5329228333205904 2307 74-75 -0.5420484743072933 2307 76-77 -0.7543612372890323 2307 78-79 -0.3520406195125574 2307 80-81 -0.22893457432638797 2307 82-83 -0.20309240390260186 2307 84-85 -0.3287667720929903 2307 86-87 -0.4168249671512285 2307 88-89 -0.4046743487655391 2307 90-91 -0.49675105971986966 2307 92-93 -0.5312223783409493 2307 94-95 -0.6794652992966874 2307 96-97 -0.6529158732298868 2307 98-99 -0.7085312561733943 2307 100-101 -1.056241393530403 2201 1 0.06046136956718584 2201 2 0.056757743733562904 2201 3 0.07110396195164981 2201 4 -0.058921539004018086 2201 5 -0.03175710983201441 2201 6 0.05079466521010545 2201 7 0.09809568655416712 2201 8 0.08717962511635591 2201 9 0.07523165046086433 2201 10-11 0.07750854587329314 2201 12-13 0.08578935232263518 2201 14-15 0.1289705234200298 2201 16-17 0.04416892575551401 2201 18-19 0.08480943357557891 2201 20-21 0.05103858538965511 2201 22-23 0.09159338458424315 2201 24-25 0.026655822444645594 2201 26-27 0.10938186973867658 2201 28-29 0.09741140256204517 2201 30-31 0.04665659112584564 2201 32-33 -0.002689978766206025 2201 34-35 -0.01281293243645365 2201 36-37 -0.01257525129852155 2201 38-39 0.018819279848379722 2201 40-41 0.006369935421524531 2201 42-43 -0.04514522993736847 2201 44-45 -0.1019470635986508 2201 46-47 -0.04365625187578104 2201 48-49 -0.029220136458789625 2201 50-51 -0.10028402451198559 2201 52-53 -0.037442135771073026 2201 54-55 0.002296861976063269 2201 56-57 -0.10901978491704511 2201 58-59 -0.10636812768140658 2201 60-61 -0.13553100579778743 2201 62-63 -0.20016515952375613 2201 64-65 -0.21758034504851764 2201 66-67 -0.301595091275324 2201 68-69 -0.461118133063394 2201 70-71 -0.4459044358262645 2201 72-73 -0.39584076512453237 2201 74-75 -0.46495513035345226 2201 76-77 -0.9707579238609334 2201 78-79 -0.40155320808578665 2201 80-81 -0.18385378170663103 2201 82-83 -0.17044799208028394 2201 84-85 -0.23600446172329015 2201 86-87 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-0.03564438665613778 1101 44-45 -0.09882066474229845 1101 46-47 -0.07722164052191971 1101 48-49 -0.06932892260577006 1101 50-51 -0.05075973392337829 1101 52-53 -0.053665569460726203 1101 54-55 -0.08216903819069898 1101 56-57 -0.1487123239817052 1101 58-59 -0.14480677656356988 1101 60-61 -0.15507627817448366 1101 62-63 -0.2351693896106326 1101 64-65 -0.28225949522242644 1101 66-67 -0.35387448940074506 1101 68-69 -0.31325808623975604 1101 70-71 -0.29219706789347555 1101 72-73 -0.34217156481674493 1101 74-75 -0.3759670303478231 1101 76-77 -0.6850193444570678 1101 78-79 -0.2600714321504185 1101 80-81 -0.133329785063367 1101 82-83 -0.10815294832129041 1101 84-85 -0.13627348904674363 1101 86-87 -0.17097828646272717 1101 88-89 -0.23590742169712797 1101 90-91 -0.2278951384887833 1101 92-93 -0.2889794200285323 1101 94-95 -0.41915362255679867 1101 96-97 -0.39833480349604855 1101 98-99 -0.4139328129975084 1101 100-101 -0.6340047379995397 2304 1 0.07932401982617421 2304 2 0.0818398193255021 2304 3 0.07226828681717734 2304 4 -0.10959726222874622 2304 5 -0.07766648203896409 2304 6 0.019711493180281536 2304 7 0.06980402494964721 2304 8 0.027282379822828773 2304 9 0.06431993317991669 2304 10-11 0.02411212540179264 2304 12-13 0.03415585940373944 2304 14-15 0.06506085802987371 2304 16-17 0.09219258699791055 2304 18-19 0.05038106141787324 2304 20-21 0.05056900213396176 2304 22-23 0.05310221058311981 2304 24-25 -0.007058765341476203 2304 26-27 0.043407349966940956 2304 28-29 -0.0219357724424043 2304 30-31 -0.011386127344451324 2304 32-33 0.012726797785219901 2304 34-35 0.0027166161073708395 2304 36-37 -0.042988741717245205 2304 38-39 -0.07356220959534454 2304 40-41 -0.039309056432664136 2304 42-43 -0.07517017948188709 2304 44-45 -0.11258364539116172 2304 46-47 -0.10747887163406489 2304 48-49 -0.06273722340174004 2304 50-51 -0.1406941296529638 2304 52-53 -0.07883344016649829 2304 54-55 -0.1532315813462901 2304 56-57 -0.0929573087856852 2304 58-59 -0.11258671381809648 2304 60-61 -0.08836486726129777 2304 62-63 -0.19977362351814776 2304 64-65 -0.20706940592149436 2304 66-67 -0.25666860071151376 2304 68-69 -0.30629443077391727 2304 70-71 -0.28639658379056243 2304 72-73 -0.2839065403521701 2304 74-75 -0.3486651712943214 2304 76-77 -0.5913977887890169 2304 78-79 -0.24286213777575938 2304 80-81 -0.135565519885958 2304 82-83 -0.12871891918330647 2304 84-85 -0.19840532637037 2304 86-87 -0.25253837049672256 2304 88-89 -0.233154248368443 2304 90-91 -0.28938430902386614 2304 92-93 -0.2782234579335565 2304 94-95 -0.29102060727746704 2304 96-97 -0.3027155213715673 2304 98-99 -0.4591502878349729 2304 100-101 -0.6581027665738617 2202 1 0.06925954862531825 2202 2 0.06536576527981453 2202 3 0.04852630641429556 2202 4 -0.0878509303588828 2202 5 -0.06341354333677174 2202 6 0.0095540590875558 2202 7 0.00994235672423116 2202 8 0.05064973213523416 2202 9 -0.002871379085043202 2202 10-11 -0.0013405821828555986 2202 12-13 0.036134590331840855 2202 14-15 0.05287553895990982 2202 16-17 -0.022468144411249114 2202 18-19 -0.00270340734770258 2202 20-21 -0.03272672778777519 2202 22-23 0.019950539658545097 2202 24-25 -0.04987100420142809 2202 26-27 -0.09391766680077751 2202 28-29 -0.047938763608534884 2202 30-31 -0.008106079359457397 2202 32-33 -0.002690925165914848 2202 34-35 -0.06601431011910819 2202 36-37 -0.06824407369164476 2202 38-39 -0.11625470623920364 2202 40-41 -0.10960304607136351 2202 42-43 -0.12496855604020851 2202 44-45 -0.17988716613331235 2202 46-47 -0.1711049578720676 2202 48-49 -0.12012998448764733 2202 50-51 -0.12717564522663594 2202 52-53 -0.13190302175950563 2202 54-55 -0.12846769304915995 2202 56-57 -0.18596707114169675 2202 58-59 -0.22098430753727172 2202 60-61 -0.15525101602578673 2202 62-63 -0.22871737618115162 2202 64-65 -0.2148875625679949 2202 66-67 -0.20757687944767156 2202 68-69 -0.2435234354484237 2202 70-71 -0.2450423259193144 2202 72-73 -0.32257240987430436 2202 74-75 -0.353779767305916 2202 76-77 -0.4320476980293151 2202 78-79 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-0.5163830121256439 1213 36-37 -0.37023756944004305 1213 38-39 -0.3583027303393962 1213 40-41 -0.2660842121261666 1213 42-43 -0.2566571823222432 1213 44-45 -0.3073821975383666 1213 46-47 -0.2845635379122484 1213 48-49 -0.22321392101368076 1213 50-51 -0.19185781205248276 1213 52-53 -0.18986222331957237 1213 54-55 -0.20022035388474535 1213 56-57 -0.14983411667811453 1213 58-59 -0.1611695080225104 1213 60-61 -0.16810037321426563 1213 62-63 -0.12221545509456178 1213 64-65 -0.06968494724198138 1213 66-67 -0.04892680533796323 1213 68-69 0.006775419385689929 1213 70-71 0.04820084001201508 1213 72-73 0.18017265186563947 1213 74-75 0.13086890843992904 1213 76-77 0.44317948480464153 1213 78-79 -0.13939021186150313 1213 80-81 -0.10802329333389338 1213 82-83 0.030674618520272645 1213 84-85 0.008196521692589442 1213 86-87 0.04189050715247333 1213 88-89 0.008718064995449026 1213 90-91 0.04171865478317471 1213 92-93 0.07805752536300048 1213 94-95 0.17238029243532793 1213 96-97 0.20389313249303598 1213 98-99 0.2745261008213973 1213 100-101 0.9145151716489082 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 4.0 7 54.0 8 737.0 9 2034.0 10 1471.0 11 636.0 12 921.0 13 1396.0 14 3268.0 15 7091.0 16 12620.0 17 19295.0 18 26411.0 19 33493.0 20 42053.0 21 52777.0 22 65542.0 23 81141.0 24 101222.0 25 126986.0 26 160391.0 27 200714.0 28 255371.0 29 326026.0 30 422140.0 31 556271.0 32 753653.0 33 1065252.0 34 1659349.0 35 3097703.0 36 6686650.0 37 8994548.0 38 1701323.0 39 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 18.741473997729212 21.635273850472693 31.857811756313048 27.765440395485047 2 26.058450764697238 20.87317170810704 33.999233367661205 19.069144159534513 3 20.018647262044638 19.41966188958922 42.87533085680801 17.686359991558128 4 19.45772341382411 18.558416805605518 42.50821089838069 19.475648882189684 5 30.309620593338053 17.974308216738496 34.56454723791636 17.151523952007093 6 29.90014655361649 18.306079662034723 35.078029142611456 16.71574464173733 7 30.804201254065976 25.199319116796005 26.92092726381042 17.0755523653276 8 27.671307783155076 20.501655115528518 33.44910927935049 18.37792782196593 9 21.472141962590882 19.25355317686031 39.80964810804633 19.464656752502478 10-11 28.62181927512988 20.385609179997502 30.57629345997745 20.416278084895172 12-13 27.105409981247668 26.90105091067637 24.267076958336684 21.726462149739277 14-15 27.126331364167143 23.347242875051176 25.764651884515626 23.761773876266055 16-17 26.42148949336523 22.77355383607888 25.363686551883173 25.441270118672715 18-19 26.176301022398178 23.958209239983287 26.172109564777784 23.693380172840744 20-21 24.265012401377035 25.149560246095053 26.97073062608817 23.61469672643974 22-23 24.740414073235737 25.131126895239138 26.393608949402925 23.734850082122204 24-25 24.806042093875348 25.295953191131677 26.08225401094904 23.815750704043936 26-27 24.961904095093637 25.128549643825 26.13934190088441 23.770204360196953 28-29 24.73062804055832 25.59977217101876 25.72346992836029 23.946129860062626 30-31 25.014774949358486 25.388182939470433 25.568339713338485 24.028702397832593 32-33 24.89009468056591 25.552295112129205 25.431257149949726 24.126353057355164 34-35 25.07821130180642 25.518867099983872 25.576331125676383 23.826590472533326 36-37 25.068825702201135 25.526074315675373 25.52549222819666 23.879607753926827 38-39 25.148172433670386 25.271343985167928 25.542355797060594 24.038127784101093 40-41 24.95139479443441 25.520748864804393 25.293517910485985 24.23433843027521 42-43 24.922153900310764 25.720065200614812 25.252511392975297 24.105269506099127 44-45 24.87287867562687 25.40936862327473 25.516881684284105 24.2008710168143 46-47 24.57039752267492 25.695112786786588 25.35174568709709 24.3827440034414 48-49 24.730516260880435 25.214775501361768 25.25785714000858 24.796851097749222 50-51 24.58548708623341 25.31027716082679 25.88518646473124 24.219049288208563 52-53 24.84665149726005 25.20583748725435 25.775682519344244 24.171828496141355 54-55 24.606261589158134 25.168950514266413 25.309761195487766 24.915026701087687 56-57 24.650111644789 25.36201693987029 25.546362353644426 24.441509061696284 58-59 24.80524841288142 25.38664211314815 25.462817982790686 24.345291491179747 60-61 24.867327030221013 25.48672807231836 25.18376853285603 24.462176364604602 62-63 24.549395257614997 25.845373852292752 25.307088418967123 24.298142471125132 64-65 24.845897744137957 25.49437789442014 25.124337044559475 24.535387316882428 66-67 24.999576593899036 25.593148472717488 25.278262867594943 24.129012065788537 68-69 24.687014107391796 25.08848889297453 25.71635573792993 24.50814126170374 70-71 24.730578401539596 25.274069894647187 25.389143107486 24.606208596327214 72-73 24.230416706947175 25.008585088558455 26.05198100516784 24.70901719932653 74-75 24.500544803850577 24.829073509845596 25.851874596292824 24.818507090011003 76-77 24.24384425611542 24.877797794305433 25.703678994430547 25.1746789551486 78-79 24.743762484042833 25.066318369262625 25.13398294221478 25.055936204479767 80-81 24.446936327372562 24.887486824145395 25.57419512123676 25.09138172724528 82-83 24.494874519146578 25.521144321247373 25.446341855132154 24.537639304473892 84-85 24.47222510820122 25.629905787596353 25.340898590473998 24.556970513728434 86-87 24.33805567038562 25.525931109810294 25.551187318748042 24.584825901056035 88-89 24.27852711147556 26.60606540950439 24.83962118301043 24.27578629600962 90-91 24.357672251416307 26.113152401292876 24.83817582446915 24.690999522821667 92-93 24.16483319649253 26.400439412842942 24.56202562999633 24.872701760668196 94-95 24.171447588043897 26.55213413895266 24.42601059675071 24.850407676252733 96-97 23.982896313269944 26.27683370827873 24.402015377728905 25.338254600722422 98-99 24.17991323190814 26.16531836655919 24.073018592900628 25.581749808632043 100-101 24.50861151666039 26.878759332858454 23.610739149217995 25.00189000126316 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 11533.0 1 7012.5 2 4497.5 3 6130.5 4 6974.0 5 5775.5 6 3040.0 7 2674.0 8 2707.0 9 2644.5 10 2399.5 11 2311.0 12 2288.0 13 2188.5 14 2168.0 15 2263.0 16 2438.0 17 2594.5 18 2791.5 19 3126.5 20 3643.5 21 4153.5 22 4613.0 23 5331.5 24 6123.0 25 7013.0 26 8336.5 27 9788.5 28 11708.0 29 14629.0 30 18950.0 31 24755.0 32 35939.0 33 56093.0 34 82900.5 35 128235.5 36 185264.5 37 255663.0 38 348496.0 39 449776.0 40 559369.5 41 683484.5 42 812226.0 43 924629.5 44 1078210.0 45 1287232.0 46 1508827.0 47 1702209.5 48 1793720.0 49 1798513.5 50 1692952.5 51 1574015.0 52 1492445.5 53 1374302.0 54 1227205.0 55 1071470.5 56 934745.5 57 739471.5 58 548892.0 59 436150.0 60 342014.0 61 268561.5 62 209242.0 63 150526.0 64 100835.5 65 66728.5 66 47142.5 67 35657.5 68 29577.5 69 25145.5 70 22587.5 71 20915.0 72 20503.0 73 20396.0 74 18918.0 75 16185.5 76 13741.0 77 11954.0 78 11141.5 79 10907.5 80 8998.0 81 6346.5 82 4710.0 83 3897.0 84 3022.0 85 2151.0 86 1789.0 87 1760.5 88 1779.0 89 1734.0 90 1502.5 91 1121.0 92 826.0 93 636.5 94 537.5 95 531.5 96 504.5 97 393.5 98 237.0 99 87.0 100 43.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11287455530486598 2 0.0 3 0.0 4 0.5655633167862095 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.840882139688847E-4 12-13 0.0 14-15 0.0 16-17 3.401543605370145E-5 18-19 0.0 20-21 0.0016081742267611075 22-23 5.310187517272283E-4 24-25 0.0 26-27 8.881808302910935E-5 28-29 5.045623014632382E-4 30-31 0.0029725711618040214 32-33 0.0013152635274097896 34-35 0.0010714862356915958 36-37 0.007829219531693618 38-39 0.015153876761923998 40-41 0.012598939565001532 42-43 0.010168725633609306 44-45 0.00852464622434707 46-47 0.01600993190260882 48-49 0.015686785260098653 50-51 0.014670101671382467 52-53 0.016833861353687366 54-55 0.018791638673222626 56-57 0.02495410183828487 58-59 0.03249985940286431 60-61 0.017814639759902422 62-63 0.008889367288700647 64-65 0.01842502786242162 66-67 0.024302139313922264 68-69 0.031076880327951133 70-71 0.03032665098832227 72-73 0.032673716076027676 74-75 0.02590464430134109 76-77 0.03313481420920007 78-79 0.017217479882515218 80-81 0.005909237141106914 82-83 0.008424489662633393 84-85 0.015286159013243948 86-87 0.028930128363673084 88-89 0.024923865895126025 90-91 0.03531936110242667 92-93 0.026131413875032428 94-95 0.0359996698235007 96-97 0.01617433984353504 98-99 0.01873683602624722 100-101 0.28844522849604604 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.6458576E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.274892133805317 #Duplication Level Percentage of deduplicated Percentage of total 1 72.7431771670658 13.29373716198417 2 11.450360870988934 4.185082196209357 3 4.096196591736534 2.2457265261853845 4 2.364806043231322 1.7286630142969341 5 1.493123284417008 1.3643333482596967 6 0.8582541580733549 0.9410701297308273 7 0.7541582470237115 0.9647512431324621 8 0.4748737745437468 0.6942613605567965 9 0.42933311491668974 0.7061414728115832 >10 3.7312237719537693 14.256288711955486 >50 0.7153031042642206 9.287350011481488 >100 0.7744313142141999 28.561471304258546 >500 0.08131761632619813 10.110073120346655 >1k 0.032277947136879426 9.966206749439287 >5k 9.116990490631508E-4 1.0457491319795742 >10k+ 2.5129505863123036E-4 0.6490945173716611 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.7385667316336298E-4 0.0 0.0 2 1.776361660582187E-4 0.0 0.0 3 1.814156589530744E-4 0.0 0.0 4 1.8519515184793015E-4 0.0 0.0 5 1.8897464474278586E-4 0.0 0.0 6 2.192105879016316E-4 0.0 0.0 7 2.3810805237591017E-4 0.0 0.0 8 2.5322602395533306E-4 0.0 0.0 9 2.721234884296116E-4 0.0 0.0 10-11 3.47713346326726E-4 0.0 0.0 12-13 5.234597659375169E-4 0.0 0.0 14-15 7.256626358122976E-4 0.0 0.0 16-17 0.0010242425745058993 0.0 0.0 18-19 0.001349278963463491 0.0 0.0 20-21 0.00176502318189762 0.0 0.0 22-23 0.0022658059904660027 0.0 0.0 24-25 0.0028138324602200813 0.0 0.0 26-27 0.0034506770130032696 0.0 0.0 28-29 0.004102639537365881 0.0 0.0 30-31 0.004905781777522721 0.0 0.0 32-33 0.005782624129129247 0.0 0.0 34-35 0.006958046419429376 0.0 0.0 36-37 0.008324333100919717 0.0 0.0 38-39 0.010066679325448202 0.0 0.0 40-41 0.012222880021963388 0.0 0.0 42-43 0.015012145778366907 0.0 0.0 44-45 0.018578097324663277 0.0 0.0 46-47 0.02313049651651699 0.0 0.0 48-49 0.028567297045766937 0.0 0.0 50-51 0.035102040260972474 0.0 0.0 52-53 0.04352652992360587 0.0 0.0 54-55 0.05386344299103625 0.0 0.0 56-57 0.06658521607512059 0.0 0.0 58-59 0.08247609395154146 0.0 0.0 60-61 0.10272094764283611 0.0 1.8897464474278585E-6 62-63 0.12852921487535837 0.0 3.779492894855717E-6 64-65 0.16149395190428995 0.0 3.779492894855717E-6 66-67 0.20177389743121474 0.0 3.779492894855717E-6 68-69 0.2505898276611712 0.0 3.779492894855717E-6 70-71 0.3100242431792248 0.0 3.779492894855717E-6 72-73 0.3773804758048959 0.0 3.779492894855717E-6 74-75 0.4572524991518818 0.0 3.779492894855717E-6 76-77 0.5500031445380885 0.0 3.779492894855717E-6 78-79 0.6622918784442519 0.0 3.779492894855717E-6 80-81 0.7941961804747164 0.0 3.779492894855717E-6 82-83 0.9522942580129785 0.0 3.779492894855717E-6 84-85 1.1457476018361685 0.0 3.779492894855717E-6 86-87 1.3759905294978838 0.0 3.779492894855717E-6 88-89 1.6449203464313422 0.0 3.779492894855717E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 436530 0.0 59.91487 1 TAACTTT 69595 0.0 59.172825 1 TTTGTGT 463685 0.0 56.472576 2 AACTTTG 73855 0.0 56.305904 2 TTGTGTT 520165 0.0 51.700077 3 ACTTTGT 151650 0.0 51.301094 1 TGTGTTT 546985 0.0 49.426357 4 GTGTTTG 602045 0.0 44.996113 5 TTGAGGG 85235 0.0 35.28278 9 TTTGAGG 170310 0.0 34.63581 8 GTTTGAG 449550 0.0 34.027935 7 TGTTTGA 804510 0.0 33.657528 6 TTGAGGC 26310 0.0 32.261223 9 GTTTGAC 168970 0.0 30.645708 7 TTTGACC 54310 0.0 30.5668 8 TTGACCC 29580 0.0 30.251528 9 TTTGAGC 111055 0.0 29.234026 8 TTGAGCC 50775 0.0 28.646593 9 TTTGACG 46135 0.0 28.193836 8 TTTGAGT 164980 0.0 26.711065 8 >>END_MODULE