FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l03n01_6957.34100000035bda.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l03n01_6957.34100000035bda.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26458576
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG4365300.059.914871
TAACTTT695950.059.1728251
TTTGTGT4636850.056.4725762
AACTTTG738550.056.3059042
TTGTGTT5201650.051.7000773
ACTTTGT1516500.051.3010941
TGTGTTT5469850.049.4263574
GTGTTTG6020450.044.9961135
TTGAGGG852350.035.282789
TTTGAGG1703100.034.635818
GTTTGAG4495500.034.0279357
TGTTTGA8045100.033.6575286
TTGAGGC263100.032.2612239
GTTTGAC1689700.030.6457087
TTTGACC543100.030.56688
TTGACCC295800.030.2515289
TTTGAGC1110550.029.2340268
TTGAGCC507750.028.6465939
TTTGACG461350.028.1938368
TTTGAGT1649800.026.7110658
Produced by FastQC (version 0.11.2)