##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l03n01_6929.34100000035b97.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15598456 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74086480097774 33.0 33.0 33.0 33.0 33.0 2 32.75312998927586 33.0 33.0 33.0 33.0 33.0 3 32.782050736303646 33.0 33.0 33.0 33.0 33.0 4 36.47116753094024 37.0 37.0 37.0 37.0 37.0 5 36.58108950014027 37.0 37.0 37.0 37.0 37.0 6 36.622703875306634 37.0 37.0 37.0 37.0 37.0 7 36.61434080398727 37.0 37.0 37.0 37.0 37.0 8 36.620843306542646 37.0 37.0 37.0 37.0 37.0 9 36.614346189135645 37.0 37.0 37.0 37.0 37.0 10-11 36.59652676521317 37.0 37.0 37.0 37.0 37.0 12-13 36.568366285740076 37.0 37.0 37.0 37.0 37.0 14-15 38.67982815094007 40.0 37.0 40.0 37.0 40.0 16-17 38.64336402910647 40.0 37.0 40.0 37.0 40.0 18-19 38.62711318992085 40.0 37.0 40.0 37.0 40.0 20-21 38.582589616562046 40.0 37.0 40.0 37.0 40.0 22-23 38.52129659499633 40.0 37.0 40.0 37.0 40.0 24-25 38.47721136630446 40.0 37.0 40.0 37.0 40.0 26-27 38.394777886990866 40.0 37.0 40.0 37.0 40.0 28-29 38.33831675391462 40.0 37.0 40.0 37.0 40.0 30-31 38.2855799638118 40.0 37.0 40.0 37.0 40.0 32-33 38.194222876930894 40.0 37.0 40.0 37.0 40.0 34-35 38.13271880883595 40.0 37.0 40.0 37.0 40.0 36-37 38.04057452865848 40.0 37.0 40.0 37.0 40.0 38-39 37.93317354615098 40.0 37.0 40.0 35.0 40.0 40-41 37.81705557909065 40.0 37.0 40.0 33.0 40.0 42-43 37.6967832264937 40.0 37.0 40.0 33.0 40.0 44-45 37.56029516639339 40.0 37.0 40.0 33.0 40.0 46-47 37.39146066764557 40.0 37.0 40.0 33.0 40.0 48-49 37.184499190176254 37.0 37.0 40.0 33.0 40.0 50-51 36.99687735119424 37.0 37.0 40.0 33.0 40.0 52-53 36.7761007243281 37.0 37.0 40.0 33.0 40.0 54-55 36.53595048125276 37.0 37.0 40.0 33.0 40.0 56-57 36.276107551926934 37.0 37.0 40.0 33.0 40.0 58-59 36.00485653836508 37.0 37.0 40.0 33.0 40.0 60-61 35.72392822084442 37.0 35.0 38.5 33.0 40.0 62-63 35.46398012085298 37.0 33.0 37.0 33.0 40.0 64-65 35.18727260569892 37.0 33.0 37.0 33.0 40.0 66-67 34.95094915163398 37.0 33.0 37.0 30.0 40.0 68-69 34.67288374567329 37.0 33.0 37.0 27.0 38.5 70-71 34.42649182713981 37.0 33.0 37.0 27.0 37.0 72-73 34.19390101815205 37.0 33.0 37.0 27.0 37.0 74-75 33.94534221848624 37.0 33.0 37.0 27.0 37.0 76-77 31.077074327100068 33.0 30.0 35.0 24.5 37.0 78-79 33.681199408454276 35.0 33.0 37.0 27.0 37.0 80-81 34.1480051615365 37.0 33.0 37.0 27.0 37.0 82-83 34.13189747754521 37.0 33.0 37.0 27.0 37.0 84-85 34.036560830123186 37.0 33.0 37.0 27.0 37.0 86-87 33.94099262132099 37.0 33.0 37.0 27.0 37.0 88-89 33.797254997545906 37.0 33.0 37.0 27.0 37.0 90-91 33.68425410181623 37.0 33.0 37.0 27.0 37.0 92-93 33.56613055163921 37.0 33.0 37.0 27.0 37.0 94-95 33.459385435327704 37.0 33.0 37.0 27.0 37.0 96-97 33.362418177799135 37.0 33.0 37.0 27.0 37.0 98-99 33.21468685746846 35.0 33.0 37.0 27.0 37.0 100-101 31.2739934324269 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.08088878113623821 2306 2 0.10014975547946392 2306 3 0.08384317436458133 2306 4 -0.0774175998028852 2306 5 -0.017071454634432826 2306 6 0.09034255476239395 2306 7 0.08745305593555486 2306 8 0.05840415656653164 2306 9 0.09679627988089834 2306 10-11 0.089151204110955 2306 12-13 0.11377269680573932 2306 14-15 0.19414066477743575 2306 16-17 0.188156971592484 2306 18-19 0.1495634210645278 2306 20-21 0.15137364171702927 2306 22-23 0.14613932838663146 2306 24-25 0.11091119313201148 2306 26-27 0.12944492859340073 2306 28-29 0.09554909813765988 2306 30-31 0.07297054501840705 2306 32-33 0.12369377219859956 2306 34-35 0.12035692968282063 2306 36-37 0.14768554687213253 2306 38-39 0.040843687374547244 2306 40-41 -0.010637891363032281 2306 42-43 0.014815544481344034 2306 44-45 0.007952645766572175 2306 46-47 -0.0335501506463558 2306 48-49 0.039490592631374 2306 50-51 0.07073266903238107 2306 52-53 0.023691129762219987 2306 54-55 0.04502880417894772 2306 56-57 -0.09449717940477598 2306 58-59 -0.11486821715502771 2306 60-61 -0.15575845928702847 2306 62-63 -0.12182709911687084 2306 64-65 -0.09699234807045087 2306 66-67 -0.13076634077538074 2306 68-69 -0.20109280047719835 2306 70-71 -0.2086594152255259 2306 72-73 -0.27632904893863497 2306 74-75 -0.2887062292395228 2306 76-77 -0.6527472073350467 2306 78-79 -0.2139019500794319 2306 80-81 -0.08119488508473438 2306 82-83 -0.07223022737885287 2306 84-85 -0.18730866772483523 2306 86-87 -0.3006829124569279 2306 88-89 -0.28843989177969576 2306 90-91 -0.2807030984568897 2306 92-93 -0.3171915728626544 2306 94-95 -0.3894522523614796 2306 96-97 -0.468332568365355 2306 98-99 -0.5496122400046701 2306 100-101 -0.8591153477781113 2307 1 0.08923661266972971 2307 2 0.08156176159114636 2307 3 0.0971315306850471 2307 4 -0.08465157989906658 2307 5 -0.021196669437507865 2307 6 0.09274085893841288 2307 7 0.12164648672469269 2307 8 0.09906777989802151 2307 9 0.04749806011795954 2307 10-11 0.09181630643926297 2307 12-13 0.1014829591934614 2307 14-15 0.15084157533896558 2307 16-17 0.12803754057907213 2307 18-19 0.07173262794093915 2307 20-21 0.08744395381451397 2307 22-23 0.10862920249727637 2307 24-25 0.08334033571939159 2307 26-27 0.040802940064281756 2307 28-29 0.0057540967947531385 2307 30-31 0.03313258666902641 2307 32-33 -0.031976574304898975 2307 34-35 0.04931161895277825 2307 36-37 0.05363855957045871 2307 38-39 0.03018844473637472 2307 40-41 -0.006018573569079422 2307 42-43 -0.12812406415889654 2307 44-45 -0.061725494911598844 2307 46-47 -0.030157795507001595 2307 48-49 -0.0572874009697486 2307 50-51 -0.15031694244953542 2307 52-53 -0.14156909964103903 2307 54-55 -0.13636464231227308 2307 56-57 -0.14200155989373542 2307 58-59 -0.24125908824633058 2307 60-61 -0.32185424684490016 2307 62-63 -0.307467473132192 2307 64-65 -0.33191329866360775 2307 66-67 -0.3962705440387353 2307 68-69 -0.3138917225760167 2307 70-71 -0.4543362287320818 2307 72-73 -0.5142290423143265 2307 74-75 -0.4939307191061246 2307 76-77 -0.7189587832560917 2307 78-79 -0.32507225104979653 2307 80-81 -0.26894079464397436 2307 82-83 -0.3203134125791891 2307 84-85 -0.3040236264469982 2307 86-87 -0.38830837209978597 2307 88-89 -0.38812489778559467 2307 90-91 -0.43586374091994884 2307 92-93 -0.5474111017109564 2307 94-95 -0.633854506301823 2307 96-97 -0.5891243295679871 2307 98-99 -0.7298463740908545 2307 100-101 -0.9881139032739021 2201 1 0.08302743937613144 2201 2 0.09653143502305284 2201 3 0.08552405207222336 2201 4 -0.06156426271082438 2201 5 -0.02876528416457802 2201 6 0.06148449896134878 2201 7 0.0687734168096128 2201 8 0.08565722831713174 2201 9 0.08716786287149603 2201 10-11 0.09408132419209636 2201 12-13 0.0949600387556373 2201 14-15 0.16803841400368924 2201 16-17 0.05644229938448575 2201 18-19 0.1100054750688102 2201 20-21 0.07274001847224554 2201 22-23 0.09665678204434869 2201 24-25 0.06343016477406138 2201 26-27 0.13816563105285695 2201 28-29 0.13474055838874932 2201 30-31 0.10324815275748023 2201 32-33 0.10039327616960492 2201 34-35 0.0857708279047884 2201 36-37 0.0439569769787056 2201 38-39 0.14099249523739132 2201 40-41 0.11105474193851705 2201 42-43 -0.004133843776166657 2201 44-45 -0.08347312097237847 2201 46-47 -0.01003795509858918 2201 48-49 -0.04773352541101161 2201 50-51 -0.04679032365301339 2201 52-53 0.0026426772359755546 2201 54-55 0.05652598782231877 2201 56-57 -0.06986948957058559 2201 58-59 -0.02286823705973262 2201 60-61 -0.14972946229440964 2201 62-63 -0.1273638228830407 2201 64-65 -0.1534083881198356 2201 66-67 -0.27301696384353846 2201 68-69 -0.3652834458444758 2201 70-71 -0.3256615077637761 2201 72-73 -0.2958506489615189 2201 74-75 -0.416975437135342 2201 76-77 -0.9497586853889004 2201 78-79 -0.31568850414474525 2201 80-81 -0.10499661632469781 2201 82-83 -0.015976079898216256 2201 84-85 -0.11381183925265503 2201 86-87 -0.18575031554597388 2201 88-89 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1101 44-45 -0.14423342118730176 1101 46-47 -0.09493655859542827 1101 48-49 -0.1112009794106612 1101 50-51 -0.08738236451911519 1101 52-53 -0.0765624707179029 1101 54-55 -0.17133490634534354 1101 56-57 -0.23968959542145996 1101 58-59 -0.18956519322005505 1101 60-61 -0.18078921777095758 1101 62-63 -0.3045811682948596 1101 64-65 -0.34744149114397516 1101 66-67 -0.38707053771907596 1101 68-69 -0.3213149139629081 1101 70-71 -0.31842056199008795 1101 72-73 -0.3517420342297015 1101 74-75 -0.4405992430780614 1101 76-77 -0.6801062864064846 1101 78-79 -0.29913408428365784 1101 80-81 -0.11540196607929687 1101 82-83 -0.07862135588214159 1101 84-85 -0.1195343727095235 1101 86-87 -0.21044764172275165 1101 88-89 -0.25782670850151845 1101 90-91 -0.26014520460613255 1101 92-93 -0.287497431545475 1101 94-95 -0.44705557480990876 1101 96-97 -0.29478887503468343 1101 98-99 -0.40269060523906575 1101 100-101 -0.6523703512265087 2304 1 0.07226123749680369 2304 2 0.05005434738085057 2304 3 0.05580786271328009 2304 4 -0.10675137060214013 2304 5 -0.07882402748534645 2304 6 0.0029266323163881225 2304 7 0.07547829352613178 2304 8 0.02463263296329643 2304 9 0.04500521949137948 2304 10-11 0.03618583134182529 2304 12-13 0.020400429847114765 2304 14-15 0.05328039067080681 2304 16-17 0.08905698273954954 2304 18-19 0.07533998651861396 2304 20-21 0.0822543354484111 2304 22-23 0.09231847152296524 2304 24-25 0.04860908751227555 2304 26-27 0.10629304835520514 2304 28-29 0.021302262531655458 2304 30-31 0.01755693038877837 2304 32-33 0.04450604186792617 2304 34-35 -0.0013243361209376303 2304 36-37 -0.06792491199128392 2304 38-39 -0.058941573148310056 2304 40-41 0.0030594154274581342 2304 42-43 -0.03133414520083733 2304 44-45 -0.04974331824940492 2304 46-47 -0.031146268779032482 2304 48-49 -0.018551027996736025 2304 50-51 -0.05559318752835907 2304 52-53 0.03911768881257416 2304 54-55 -0.055875358583982404 2304 56-57 -0.006327777893837094 2304 58-59 -0.13042259572865333 2304 60-61 -0.09398696473426327 2304 62-63 -0.11565285694043581 2304 64-65 -0.14757846747214387 2304 66-67 -0.21263155089885544 2304 68-69 -0.2689350433082609 2304 70-71 -0.20202294791382513 2304 72-73 -0.27567566201011573 2304 74-75 -0.3674263655813661 2304 76-77 -0.6014982130545867 2304 78-79 -0.21417090605076083 2304 80-81 -0.07422625343424727 2304 82-83 -0.08613557405688965 2304 84-85 -0.18233106457974912 2304 86-87 -0.24477404340287023 2304 88-89 -0.28560030704125694 2304 90-91 -0.2573203543271845 2304 92-93 -0.2109316024648038 2304 94-95 -0.27494846151089547 2304 96-97 -0.2839968069456802 2304 98-99 -0.5116042764772644 2304 100-101 -0.6335102068853047 2202 1 0.06865651388774552 2202 2 0.06722005454649604 2202 3 0.05349514563589253 2202 4 -0.13058076374206706 2202 5 -0.08129604383891831 2202 6 0.002925355147098685 2202 7 0.04005083142833854 2202 8 0.03413212356196027 2202 9 -0.009832961109374594 2202 10-11 0.00556563446412639 2202 12-13 0.03303578890023573 2202 14-15 0.026001347502074168 2202 16-17 -0.029239829701324993 2202 18-19 -6.289915753470154E-4 2202 20-21 0.003453675937109324 2202 22-23 0.0340061539315073 2202 24-25 -0.010906599485132062 2202 26-27 -0.08256948827393273 2202 28-29 0.007325623440188167 2202 30-31 0.07725429866003708 2202 32-33 0.007843896065203637 2202 34-35 -0.007589695067160562 2202 36-37 0.0012397495984259876 2202 38-39 -0.048189628847268295 2202 40-41 -0.0957900978590942 2202 42-43 -0.08023289730552818 2202 44-45 -0.10041119422195521 2202 46-47 -0.10618508920692449 2202 48-49 -0.08595300675138873 2202 50-51 -0.048805056385589296 2202 52-53 -0.09613618705723326 2202 54-55 -0.060837224358664344 2202 56-57 -0.11526332571889242 2202 58-59 -0.08893063714916849 2202 60-61 -0.060246454062699684 2202 62-63 -0.18206451704828908 2202 64-65 -0.158066053496988 2202 66-67 -0.1808157107010544 2202 68-69 -0.1926555238657528 2202 70-71 -0.25822150302224856 2202 72-73 -0.30707655621152696 2202 74-75 -0.31108722724554383 2202 76-77 -0.4264481283088841 2202 78-79 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40-41 -0.2972766187954434 1213 42-43 -0.2910245551340793 1213 44-45 -0.27227831408480796 1213 46-47 -0.2970120529857212 1213 48-49 -0.25854847545004844 1213 50-51 -0.20731019154075625 1213 52-53 -0.17921171062189245 1213 54-55 -0.22630889617161642 1213 56-57 -0.20159327940842076 1213 58-59 -0.18494427401434876 1213 60-61 -0.22296031794187599 1213 62-63 -0.19645495483864295 1213 64-65 -0.12531798338515188 1213 66-67 -0.07303301905722037 1213 68-69 -0.08565160109112924 1213 70-71 -0.02171962212594991 1213 72-73 0.07048620781549886 1213 74-75 0.05478420842782583 1213 76-77 0.3649129966863143 1213 78-79 -0.23019857487310702 1213 80-81 -0.18174490026657963 1213 82-83 -0.05656984482617844 1213 84-85 -0.14199945536102376 1213 86-87 -0.11437549564613647 1213 88-89 -0.11270827701671493 1213 90-91 -0.06831255833068184 1213 92-93 -0.021971859889944767 1213 94-95 0.050356196012998566 1213 96-97 0.12758475976746553 1213 98-99 0.18751597188929026 1213 100-101 0.8363168330315105 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 2.0 7 46.0 8 491.0 9 1375.0 10 897.0 11 362.0 12 449.0 13 583.0 14 1252.0 15 2598.0 16 4419.0 17 7060.0 18 9963.0 19 13371.0 20 17336.0 21 22649.0 22 28844.0 23 36975.0 24 47864.0 25 61743.0 26 79584.0 27 102862.0 28 131616.0 29 170046.0 30 221053.0 31 292266.0 32 398737.0 33 576537.0 34 919410.0 35 1755685.0 36 3884196.0 37 5601797.0 38 1206253.0 39 134.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 20.90500938536904 24.491371788850184 29.839819098503952 24.763799727276822 2 26.10107051621007 25.04773549382067 28.360960854074275 20.49023313589499 3 21.82024938878566 22.856210896770808 33.06636246561839 22.25717724882514 4 21.76678546840548 23.643119215539727 31.939956443230937 22.650138872823856 5 26.07981200190583 22.787313052009765 29.784358144165036 21.34851680191937 6 27.346501474248473 23.343143706018083 28.503045429624574 20.807309390108866 7 25.876663690303708 27.00150579006025 25.706390427360247 21.4154400922758 8 25.062256161763703 24.3890549167174 28.55719181436932 21.99149710714958 9 22.85355678792824 23.407438531095643 31.11720801084415 22.621796670131967 10-11 25.441710996410226 24.229432094722586 27.633641623429455 22.69521528543773 12-13 24.936923244198013 26.092136939707366 25.86254370304343 23.10839611305119 14-15 24.63782953902617 25.08392176764162 26.806621116859258 23.471627576472955 16-17 24.346357351327942 25.0227610748901 26.565379041975994 24.06550253180596 18-19 24.252297791525006 25.181155750287083 26.834338603769503 23.732207854418412 20-21 23.76982385074989 25.450524942216063 27.027621084057625 23.752030122976425 22-23 23.96292729448245 25.680484301699103 26.553450179685374 23.80313822413308 24-25 24.257266231991167 25.54147025833839 26.2939165260972 23.907346983573248 26-27 24.11811450754417 25.6067249547623 26.497096071693583 23.778064465999947 28-29 24.094220580854717 25.90635479957512 26.27218135632137 23.72724326324879 30-31 24.16056814552632 25.806743317426033 26.164004424805 23.868684112242644 32-33 24.137012717231563 26.082827712898865 26.05029841336963 23.72986115649994 34-35 23.970874744006288 26.062486298572534 26.147502063287924 23.819136894133255 36-37 24.052291235786257 25.8794512483616 26.214239256722216 23.854018259129926 38-39 24.159211146119034 25.81337315894624 26.181717759756694 23.84569793517803 40-41 23.897268725387548 25.99135460719636 26.091361663312103 24.020015004103993 42-43 23.923995850425538 25.875661514748554 26.07720952658177 24.123133108244136 44-45 24.113880645440602 25.791962225717253 26.103883060317784 23.990274068524357 46-47 23.95286443668491 25.87562378303099 26.121621641117432 24.049890139166674 48-49 23.97552670916106 25.663946792309616 26.085053155817917 24.275473342711415 50-51 23.931549866349457 25.821501917452544 26.28728552267625 23.95966269352174 52-53 23.976656783499127 25.867183270320805 26.338970108347432 23.81718983783263 54-55 23.865616256251514 25.886311039363584 26.070833067229703 24.177239637155196 56-57 23.97606711196877 26.004813792578947 26.17938655630367 23.839732539148617 58-59 23.845885335624768 26.157077505157233 26.182838828579975 23.814198330638025 60-61 24.15986983999852 26.00398273240912 25.85431527386183 23.981832153730533 62-63 23.918071688330606 26.143146809340244 26.209617919187867 23.729163583141286 64-65 24.030587038115815 26.073991239136685 26.082189119357867 23.813232603389633 66-67 24.13042872557165 26.08168599339402 26.137856403883685 23.650028877150643 68-69 24.064535806596258 25.901555460130126 26.349176391338652 23.68473234193496 70-71 23.957844046288706 26.14937865535399 26.17310010892694 23.719677189430364 72-73 23.909017048048558 25.93648254687006 26.23986551028807 23.914634894793313 74-75 23.985858968220512 25.964774903891318 26.216227455672687 23.833138672215483 76-77 24.005401117291207 25.988105613583468 26.060214569677427 23.946278699447895 78-79 23.909229353992703 25.924036508545196 26.073831613092548 24.092902524369556 80-81 23.935908287822546 25.912889523748767 26.179727286292977 23.97147490213571 82-83 23.76406308499515 26.30635873005625 26.156263370888322 23.77331481406028 84-85 23.902560975852158 26.12787637626107 25.975346578337007 23.99421606954977 86-87 23.837300368227858 26.231655180381182 26.02909314611891 23.901951305272053 88-89 23.91275874651962 26.77343199446391 25.677868035671935 23.63594122334454 90-91 23.889013198901516 26.251654857001693 25.80354879326189 24.055783150834905 92-93 23.802735122284567 26.429544843232144 25.703660532438928 24.064059502044362 94-95 23.864178321532002 26.66374456631549 25.542566792060022 23.929510320092483 96-97 23.84341935932089 26.372774919264373 25.60840618062139 24.175399540793343 98-99 24.10898028199359 26.339363747876774 25.509047866068112 24.04260810406152 100-101 24.205559466748962 26.721222992673745 25.179507703979453 23.89370983659784 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2056.0 1 1552.0 2 1173.5 3 1272.0 4 1287.0 5 1277.0 6 1319.0 7 1402.0 8 1527.0 9 1726.5 10 1840.0 11 2030.5 12 2262.5 13 2569.5 14 2922.5 15 3198.5 16 3631.5 17 4124.5 18 4596.5 19 5286.5 20 6032.0 21 6914.0 22 8071.5 23 9537.0 24 11481.5 25 14347.0 26 17983.5 27 21981.5 28 26994.5 29 34037.0 30 44025.0 31 57415.5 32 75277.0 33 97957.0 34 125807.0 35 160076.0 36 198615.0 37 241940.5 38 286433.5 39 331753.0 40 382951.0 41 440597.5 42 495393.0 43 533235.5 44 582741.0 45 681297.5 46 784581.0 47 867192.0 48 946990.5 49 971654.5 50 933765.0 51 883258.0 52 811665.0 53 750034.5 54 687711.0 55 621898.0 56 578382.5 57 478095.5 58 353135.5 59 268666.0 60 205463.5 61 157336.0 62 117469.0 63 81839.5 64 53789.0 65 33737.5 66 20806.5 67 13691.5 68 10218.0 69 7720.0 70 5814.0 71 4352.0 72 3160.5 73 2294.0 74 1622.0 75 1110.0 76 718.5 77 456.5 78 303.0 79 203.0 80 136.5 81 91.5 82 59.0 83 41.0 84 28.0 85 15.5 86 7.0 87 4.5 88 2.5 89 4.0 90 4.5 91 2.5 92 1.5 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11425489804888381 2 0.0 3 0.0 4 0.5678895398365069 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.82759883414102E-4 12-13 0.0 14-15 0.0 16-17 3.525990008241841E-5 18-19 0.0 20-21 0.0016860643130320078 22-23 5.12871273926086E-4 24-25 0.0 26-27 1.0577970024725525E-4 28-29 5.160767193881241E-4 30-31 0.0030804330890185543 32-33 0.001349492539518014 34-35 0.001105878684403123 36-37 0.008157858700886806 38-39 0.015786818900537337 40-41 0.013183997185362449 42-43 0.010744653188751502 44-45 0.008907932939003707 46-47 0.01659459115697092 48-49 0.01612339067405133 50-51 0.01520983871737049 52-53 0.017613922813899015 54-55 0.019412177718102358 56-57 0.02627824189778783 58-59 0.03406106347961618 60-61 0.01847298219772521 62-63 0.00932464084906865 64-65 0.01898264802618926 66-67 0.02530058103186623 68-69 0.032875048658662115 70-71 0.03190379868366459 72-73 0.033932845661134664 74-75 0.027320011672950197 76-77 0.034529058517073746 78-79 0.018075506960432492 80-81 0.006176893405347299 82-83 0.008792536902370337 84-85 0.0159438857281772 86-87 0.03022414526155666 88-89 0.026076298833679436 90-91 0.03666388519479107 92-93 0.027153328508924216 94-95 0.03712226389586251 96-97 0.016960011939643256 98-99 0.019655791573217247 100-101 0.2872463787441526 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5598456E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.649361609082916 #Duplication Level Percentage of deduplicated Percentage of total 1 83.52203987713955 30.61029441785531 2 8.26639882288163 6.059164793293725 3 2.5876796259465302 2.8451041901931244 4 1.2724356079284607 1.8653581087697362 5 0.685341284256884 1.2558660276181914 6 0.4951013645369157 1.0887089365238283 7 0.42448632640164025 1.0890007012083442 8 0.285788511977752 0.8379173215355487 9 0.20328183975997344 0.6705134688530625 >10 1.6245658384719648 12.46180465797279 >50 0.2781421339589018 7.248792021598159 >100 0.32305370950529316 22.972020473401134 >500 0.021709220313428625 5.33968748815958 >1k 0.009975836921016407 5.655767393017419 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.334158201298898E-5 0.0 0.0 2 8.334158201298898E-5 6.410890924076075E-6 0.0 3 8.334158201298898E-5 6.410890924076075E-6 0.0 4 8.334158201298898E-5 6.410890924076075E-6 0.0 5 8.334158201298898E-5 6.410890924076075E-6 0.0 6 8.334158201298898E-5 6.410890924076075E-6 0.0 7 8.975247293706506E-5 6.410890924076075E-6 0.0 8 1.0898514570929328E-4 6.410890924076075E-6 0.0 9 1.282178184815215E-4 6.410890924076075E-6 0.0 10-11 1.4745049125374974E-4 6.410890924076075E-6 0.0 12-13 2.5964108242508105E-4 6.410890924076075E-6 0.0 14-15 3.8785890090660256E-4 6.410890924076075E-6 0.0 16-17 5.737747377048087E-4 6.410890924076075E-6 0.0 18-19 7.885395836613573E-4 1.282178184815215E-5 0.0 20-21 9.968935386938298E-4 1.282178184815215E-5 0.0 22-23 0.001285383630277253 1.282178184815215E-5 0.0 24-25 0.0015834900582467905 1.282178184815215E-5 0.0 26-27 0.001875185595292252 1.9232672772228225E-5 0.0 28-29 0.0022374009325025505 1.9232672772228225E-5 0.0 30-31 0.002647697951643419 2.884900915834234E-5 0.0 32-33 0.003160569225569505 3.205445462038038E-5 0.0 34-35 0.0038882053454521396 3.525990008241841E-5 0.0 36-37 0.004622252356258851 3.846534554445645E-5 0.0 38-39 0.005558242431173957 3.846534554445645E-5 0.0 40-41 0.0065775740881020535 3.846534554445645E-5 0.0 42-43 0.007927066627620067 3.846534554445645E-5 0.0 44-45 0.009629158167962264 3.846534554445645E-5 0.0 46-47 0.011821682863996283 4.487623646853253E-5 3.2054454620380375E-6 48-49 0.014613625861431414 4.487623646853253E-5 6.410890924076075E-6 50-51 0.01779022231431111 4.487623646853253E-5 6.410890924076075E-6 52-53 0.02160790785959841 4.487623646853253E-5 6.410890924076075E-6 54-55 0.02654108842567495 4.487623646853253E-5 9.616336386114112E-6 56-57 0.03247757342136939 4.487623646853253E-5 1.282178184815215E-5 58-59 0.03970905838372721 4.487623646853253E-5 1.282178184815215E-5 60-61 0.04862660765911703 4.487623646853253E-5 1.282178184815215E-5 62-63 0.05981361232162978 4.487623646853253E-5 1.282178184815215E-5 64-65 0.07384384710897027 4.487623646853253E-5 1.282178184815215E-5 66-67 0.09211809168804913 4.487623646853253E-5 1.282178184815215E-5 68-69 0.11508510842355166 5.12871273926086E-5 1.282178184815215E-5 70-71 0.14384436510895693 5.12871273926086E-5 1.282178184815215E-5 72-73 0.1802966909032535 5.12871273926086E-5 1.282178184815215E-5 74-75 0.22640061298374659 5.12871273926086E-5 1.282178184815215E-5 76-77 0.28312738132543375 5.12871273926086E-5 1.282178184815215E-5 78-79 0.35325291169843986 5.12871273926086E-5 1.282178184815215E-5 80-81 0.43920372631752785 5.449257285464664E-5 1.282178184815215E-5 82-83 0.5442718176722106 5.769801831668468E-5 1.282178184815215E-5 84-85 0.6740955643302132 5.769801831668468E-5 1.282178184815215E-5 86-87 0.8311816246428493 5.769801831668468E-5 1.282178184815215E-5 88-89 1.021187609850616 5.769801831668468E-5 1.282178184815215E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 72220 0.0 66.55919 1 TTTGTGT 78985 0.0 60.9374 2 TTGTGTT 89945 0.0 55.184124 3 TGTGTTT 99065 0.0 50.431885 4 ACTTTGT 22580 0.0 49.63819 1 GTGTTTG 107305 0.0 46.614243 5 GTTTGAG 89725 0.0 34.694374 2 TGTTTGA 174550 0.0 33.98748 1 AACTTTG 11080 0.0 30.680693 2 TTTGAGG 33940 0.0 30.490623 8 TTTGAGT 40625 0.0 29.604635 3 TAACTTT 11620 0.0 29.204466 1 TTGAGGG 18520 0.0 27.09291 9 TTGAGTC 17100 0.0 24.514673 4 TTTGAGC 27180 0.0 24.012865 3 GTTTGAT 61915 0.0 23.103132 2 GTTTGAA 21960 0.0 22.546097 2 GTTTGAC 42050 0.0 22.197582 2 TTGAGGC 8280 0.0 20.811237 9 TTTGAGA 17165 0.0 19.3037 3 >>END_MODULE