FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l03n01_6929.34100000035b97.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l03n01_6929.34100000035b97.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15598456
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG722200.066.559191
TTTGTGT789850.060.93742
TTGTGTT899450.055.1841243
TGTGTTT990650.050.4318854
ACTTTGT225800.049.638191
GTGTTTG1073050.046.6142435
GTTTGAG897250.034.6943742
TGTTTGA1745500.033.987481
AACTTTG110800.030.6806932
TTTGAGG339400.030.4906238
TTTGAGT406250.029.6046353
TAACTTT116200.029.2044661
TTGAGGG185200.027.092919
TTGAGTC171000.024.5146734
TTTGAGC271800.024.0128653
GTTTGAT619150.023.1031322
GTTTGAA219600.022.5460972
GTTTGAC420500.022.1975822
TTGAGGC82800.020.8112379
TTTGAGA171650.019.30373