FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l03n01_6777.34100000035b47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l03n01_6777.34100000035b47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12714603
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1041050.068.560821
TTTGTGT1176800.060.7313082
TTGTGTT1297950.056.4712373
TGTGTTT1405450.052.4579624
ACTTTGT278700.051.1343881
GTGTTTG1471900.050.1830565
AACTTTG130050.048.891052
TAACTTT134750.047.0635341
TTTGAGG408050.035.2729348
GTTTGAG1127750.034.4449427
TGTTTGA2146850.034.4081736
TTGAGGG185950.033.0086759
TTGAGGC85000.030.269569
TTTGAGC297550.029.8815828
TTGAGTC137550.029.810654
GTTTGAC433400.027.8427687
TTTGAGT484450.027.1461413
GTTTGAA217700.025.0843622
TTGAGCC164450.024.5941319
GTTTGAT763650.024.3119187