FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l03n01_30082.34100000035d38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l03n01_30082.34100000035d38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15022337
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGAGTAGCGAAAGACAGGTGAGAATCCTGTCCACCGTAAGACTAAG158100.1052432787255405No Hit
CTTTGTGTTTGAGGGGTCCATCCCGGATTACCAATCTCAGATAAACTCCG151600.10091638870836142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1482650.078.5170441
TTTGTGT1580200.073.762912
TTGTGTT1790950.067.1277853
TGTGTTT1868500.064.669254
GTGTTTG2032900.059.5320635
ACTTTGT229450.048.9398731
TTTGAGT748150.041.8613743
GTTTGAA263500.041.1891982
TGTTTGA3503100.040.915741
GTTTGAG1960500.040.242652
TTGAGGG410800.039.2549139
TTGAGTC275850.039.0684364
TTTGAGG761350.038.6265458
TTTGATT358000.037.5189973
TGAGTCT51050.037.2887275
TGAGTGG112850.034.535885
TTGATTC104150.034.522574
GTTTGAT967050.034.410112
TTGAGGC125050.034.3553059
TTGAGTG329600.034.2474064
Produced by FastQC (version 0.11.2)