##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l03n01_30082.34100000035d38.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15022337 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.740315704540514 33.0 33.0 33.0 33.0 33.0 2 32.7548988549518 33.0 33.0 33.0 33.0 33.0 3 32.78182788736533 33.0 33.0 33.0 33.0 33.0 4 36.475311264818515 37.0 37.0 37.0 37.0 37.0 5 36.58476760307001 37.0 37.0 37.0 37.0 37.0 6 36.61871205525478 37.0 37.0 37.0 37.0 37.0 7 36.60542990082036 37.0 37.0 37.0 37.0 37.0 8 36.6163799946706 37.0 37.0 37.0 37.0 37.0 9 36.607431120737076 37.0 37.0 37.0 37.0 37.0 10-11 36.592672032320934 37.0 37.0 37.0 37.0 37.0 12-13 36.55515519988668 37.0 37.0 37.0 37.0 37.0 14-15 38.629629098322056 40.0 37.0 40.0 37.0 40.0 16-17 38.59860077030625 40.0 37.0 40.0 37.0 40.0 18-19 38.574261082014075 40.0 37.0 40.0 37.0 40.0 20-21 38.5201428379619 40.0 37.0 40.0 37.0 40.0 22-23 38.45998944771376 40.0 37.0 40.0 37.0 40.0 24-25 38.41732481437475 40.0 37.0 40.0 37.0 40.0 26-27 38.33008825457716 40.0 37.0 40.0 37.0 40.0 28-29 38.276307308243716 40.0 37.0 40.0 37.0 40.0 30-31 38.22569491018608 40.0 37.0 40.0 37.0 40.0 32-33 38.126333505898586 40.0 37.0 40.0 37.0 40.0 34-35 38.060627883664175 40.0 37.0 40.0 37.0 40.0 36-37 37.959442695234436 40.0 37.0 40.0 35.0 40.0 38-39 37.8439259816898 40.0 37.0 40.0 33.0 40.0 40-41 37.72939636489316 40.0 37.0 40.0 33.0 40.0 42-43 37.60724852597835 40.0 37.0 40.0 33.0 40.0 44-45 37.4673608706821 40.0 37.0 40.0 33.0 40.0 46-47 37.30828369114606 40.0 37.0 40.0 33.0 40.0 48-49 37.10329930689213 37.0 37.0 40.0 33.0 40.0 50-51 36.90887815923715 37.0 37.0 40.0 33.0 40.0 52-53 36.68396744794102 37.0 37.0 40.0 33.0 40.0 54-55 36.44166892940825 37.0 37.0 40.0 33.0 40.0 56-57 36.17706233058145 37.0 37.0 40.0 33.0 40.0 58-59 35.89975025190821 37.0 37.0 40.0 33.0 40.0 60-61 35.61153038305558 37.0 33.0 37.0 33.0 40.0 62-63 35.34792176476935 37.0 33.0 37.0 33.0 40.0 64-65 35.065517435802434 37.0 33.0 37.0 30.0 40.0 66-67 34.823155811242955 37.0 33.0 37.0 27.0 40.0 68-69 34.541883729542214 37.0 33.0 37.0 27.0 37.0 70-71 34.294384555478956 37.0 33.0 37.0 27.0 37.0 72-73 34.070271522999384 37.0 33.0 37.0 27.0 37.0 74-75 33.81620942200937 37.0 33.0 37.0 27.0 37.0 76-77 30.94187339160345 33.0 30.0 33.0 24.5 37.0 78-79 33.55968165938495 35.0 33.0 37.0 27.0 37.0 80-81 34.031958010261654 37.0 33.0 37.0 27.0 37.0 82-83 34.00928630478733 37.0 33.0 37.0 27.0 37.0 84-85 33.920573809521116 37.0 33.0 37.0 27.0 37.0 86-87 33.8239461010627 37.0 33.0 37.0 27.0 37.0 88-89 33.6725398651355 37.0 33.0 37.0 27.0 37.0 90-91 33.560061959733694 37.0 33.0 37.0 27.0 37.0 92-93 33.44401666664781 37.0 33.0 37.0 27.0 37.0 94-95 33.33448730380633 37.0 33.0 37.0 27.0 37.0 96-97 33.24000995983515 37.0 33.0 37.0 27.0 37.0 98-99 33.09680301407165 35.0 33.0 37.0 27.0 37.0 100-101 31.13280713247213 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.09345559960507188 2306 2 0.10273243801742638 2306 3 0.08767473951764515 2306 4 -0.07601038879778343 2306 5 -0.018704610707160896 2306 6 0.0993355928435875 2306 7 0.12015604413920045 2306 8 0.07438644257147331 2306 9 0.10571729862811452 2306 10-11 0.08867156682619992 2306 12-13 0.10603029603444014 2306 14-15 0.18248232111590568 2306 16-17 0.16354218145171018 2306 18-19 0.1318131925700996 2306 20-21 0.1584624115397446 2306 22-23 0.1829580699760811 2306 24-25 0.11401106889150725 2306 26-27 0.10113470320767703 2306 28-29 0.14656257406458195 2306 30-31 0.10705424379977302 2306 32-33 0.13520688850712048 2306 34-35 0.12119778700004957 2306 36-37 0.13266774247802005 2306 38-39 0.05643296062083891 2306 40-41 0.02840317043816043 2306 42-43 0.08688253687144254 2306 44-45 -0.0053835653345828405 2306 46-47 -0.01166835211936501 2306 48-49 0.061609740030235116 2306 50-51 -0.009497957479347008 2306 52-53 -0.03590875570041874 2306 54-55 0.012082289811345959 2306 56-57 -0.11092259985542086 2306 58-59 -0.16321698726779488 2306 60-61 -0.08867597074008415 2306 62-63 -0.11501843118147548 2306 64-65 -0.125756074203359 2306 66-67 -0.18885770686402026 2306 68-69 -0.19785387715703706 2306 70-71 -0.26972770635759247 2306 72-73 -0.3649053547612411 2306 74-75 -0.34316487481063973 2306 76-77 -0.6349974898550599 2306 78-79 -0.2895037622938972 2306 80-81 -0.09955271973392854 2306 82-83 -0.16884686474752897 2306 84-85 -0.309283136530631 2306 86-87 -0.3991892980074425 2306 88-89 -0.38969412442646245 2306 90-91 -0.3233492353109497 2306 92-93 -0.40670185886668264 2306 94-95 -0.44070788899570346 2306 96-97 -0.5693377106640156 2306 98-99 -0.5578963992652533 2306 100-101 -0.9520269918560444 2307 1 0.10188602659336254 2307 2 0.09314439830610155 2307 3 0.08905365938502285 2307 4 -0.06836046582154864 2307 5 -0.025534388888608817 2307 6 0.06727664899722896 2307 7 0.07331624537908255 2307 8 0.09668809544647416 2307 9 0.06917565951541604 2307 10-11 0.04544360762266564 2307 12-13 0.06854175871470147 2307 14-15 0.1480057988609076 2307 16-17 0.08859313231240407 2307 18-19 0.06535585406641786 2307 20-21 0.05922248793463325 2307 22-23 0.062103398783754926 2307 24-25 0.07662971528220908 2307 26-27 0.00841006973131897 2307 28-29 -0.011030018200827385 2307 30-31 0.034875505205612 2307 32-33 -0.05270978040778829 2307 34-35 0.06606291371343076 2307 36-37 0.02327879961261914 2307 38-39 0.01324061525041742 2307 40-41 -0.050778525067109115 2307 42-43 -0.10128332939521556 2307 44-45 -0.09327114391561508 2307 46-47 -0.12065648616317759 2307 48-49 -0.13126229333150263 2307 50-51 -0.13181345239679132 2307 52-53 -0.15540515906936747 2307 54-55 -0.09303020366296977 2307 56-57 -0.15829437378132383 2307 58-59 -0.2561563343553743 2307 60-61 -0.26190632653573687 2307 62-63 -0.28348175007143794 2307 64-65 -0.30228598200006473 2307 66-67 -0.40866088102668385 2307 68-69 -0.4777270866586534 2307 70-71 -0.5562866669245423 2307 72-73 -0.5653504170533026 2307 74-75 -0.6011317449846985 2307 76-77 -0.7767867878814982 2307 78-79 -0.3815737936218113 2307 80-81 -0.25176155492187746 2307 82-83 -0.3032489186764664 2307 84-85 -0.3847168998864703 2307 86-87 -0.5067593369674697 2307 88-89 -0.5056910329706668 2307 90-91 -0.5355333929377935 2307 92-93 -0.5504896659349185 2307 94-95 -0.6809358630496121 2307 96-97 -0.6205945996327813 2307 98-99 -0.7051070458642812 2307 100-101 -1.0839985349788428 2201 1 0.05124096787073995 2201 2 0.061726297327233226 2201 3 0.04740925645460692 2201 4 -0.13449692289166393 2201 5 -0.06746821794778413 2201 6 0.023719152398861354 2201 7 0.07587469673635638 2201 8 0.09188463878196274 2201 9 0.06475485089494981 2201 10-11 0.07657649585127757 2201 12-13 0.09720945413940285 2201 14-15 0.14727215806866667 2201 16-17 0.012589968658680561 2201 18-19 0.08172247737901017 2201 20-21 0.01772866748804347 2201 22-23 0.08499322598512293 2201 24-25 0.04875622338593644 2201 26-27 0.09710816479181972 2201 28-29 0.06654413476940135 2201 30-31 0.029270129034294712 2201 32-33 0.047720331528267934 2201 34-35 0.029853278099302827 2201 36-37 0.0036642183743680334 2201 38-39 0.02419641789184368 2201 40-41 0.03875167194548368 2201 42-43 -0.0026166958884488167 2201 44-45 -0.09886511099070106 2201 46-47 -0.02399120011683209 2201 48-49 -0.07040316977402483 2201 50-51 -0.09913339692732848 2201 52-53 -0.0677720595416531 2201 54-55 6.709303059295735E-4 2201 56-57 -0.16264954895616057 2201 58-59 -0.15886944674686276 2201 60-61 -0.1464197321724967 2201 62-63 -0.1758956653995014 2201 64-65 -0.2203737052145982 2201 66-67 -0.2911902983224408 2201 68-69 -0.4076913346399067 2201 70-71 -0.42251814264108845 2201 72-73 -0.37555867988277214 2201 74-75 -0.4894028320867605 2201 76-77 -1.0391001337483736 2201 78-79 -0.5004145083937033 2201 80-81 -0.2508191064187457 2201 82-83 -0.17091646766612456 2201 84-85 -0.28465193765035934 2201 86-87 -0.3314073022324564 2201 88-89 -0.2765154620307655 2201 90-91 -0.3213379213685599 2201 92-93 -0.3465363679308524 2201 94-95 -0.4999557365374585 2201 96-97 -0.46851413151895827 2201 98-99 -0.525577845700397 2201 100-101 -0.6879616637109045 1101 1 0.05237927004665721 1101 2 0.07569218043149561 1101 3 0.07496334363317914 1101 4 -0.07811288083690471 1101 5 -0.047135546381454674 1101 6 0.05451421647899224 1101 7 0.010577301650464221 1101 8 0.04686428848380331 1101 9 0.04417236378841949 1101 10-11 0.04163842511291449 1101 12-13 0.024194042141843397 1101 14-15 0.07196071745683952 1101 16-17 0.05936669645053172 1101 18-19 0.08774489420324016 1101 20-21 0.07990424359147141 1101 22-23 0.07852469747810176 1101 24-25 0.06941280210286749 1101 26-27 0.040770788096835986 1101 28-29 0.009950078397103823 1101 30-31 0.004783573896069981 1101 32-33 -0.07682641323869888 1101 34-35 -0.03105437843190373 1101 36-37 -0.09098428421226856 1101 38-39 -0.05917745503300864 1101 40-41 0.0011252698200863165 1101 42-43 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0.07574200864605274 2304 4 -0.06026414280798065 2304 5 -0.04221475191588553 2304 6 0.02415580683901908 2304 7 0.06602842839450318 2304 8 0.0075047802459238255 2304 9 0.027889436276922197 2304 10-11 0.03211356491654982 2304 12-13 0.01216708741556971 2304 14-15 0.05497301531409704 2304 16-17 0.07675894155121199 2304 18-19 0.05172628215292008 2304 20-21 0.04635042675320733 2304 22-23 0.0541567562232288 2304 24-25 0.06657553639771407 2304 26-27 0.040928747305791546 2304 28-29 -0.03551708798144659 2304 30-31 0.021156474413807302 2304 32-33 0.061035357283209635 2304 34-35 0.01610426871131665 2304 36-37 -0.030712644352703933 2304 38-39 -0.053216408572495766 2304 40-41 0.015583543156715507 2304 42-43 -0.03077885429703997 2304 44-45 -0.042487374813163115 2304 46-47 -0.06200426055674768 2304 48-49 0.009753261008242475 2304 50-51 -0.029941951928002197 2304 52-53 0.017283577553961038 2304 54-55 -0.0800169814408207 2304 56-57 -0.06500843477008544 2304 58-59 -0.07446985963433406 2304 60-61 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2202 16-17 -0.02371391517014132 2202 18-19 -0.0319286116331412 2202 20-21 -0.017214570609169755 2202 22-23 -0.006130176607364035 2202 24-25 -0.031951097237957526 2202 26-27 -0.08757511205828905 2202 28-29 -0.06406514194996049 2202 30-31 -0.030394360238339857 2202 32-33 -0.011357065258074783 2202 34-35 -0.07399792811816042 2202 36-37 -0.07194276157058965 2202 38-39 -0.1473647108482652 2202 40-41 -0.15652110353551763 2202 42-43 -0.1371836232269601 2202 44-45 -0.14942656635844997 2202 46-47 -0.21340999384869264 2202 48-49 -0.16899015610324142 2202 50-51 -0.2110697062673097 2202 52-53 -0.20575535073965057 2202 54-55 -0.183174468785964 2202 56-57 -0.21033855952403968 2202 58-59 -0.17742438389729642 2202 60-61 -0.17569107447121723 2202 62-63 -0.24821021894103268 2202 64-65 -0.26604025052358793 2202 66-67 -0.22394119821681358 2202 68-69 -0.31325811454490093 2202 70-71 -0.31391033024672055 2202 72-73 -0.31356843311021265 2202 74-75 -0.4030035706384467 2202 76-77 -0.5255248284046701 2202 78-79 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-0.2728272069513338 1213 46-47 -0.31888827424981514 1213 48-49 -0.27333324343987897 1213 50-51 -0.22731384920998465 1213 52-53 -0.21287461211261416 1213 54-55 -0.20583930259599015 1213 56-57 -0.25662997967973666 1213 58-59 -0.1982504125344775 1213 60-61 -0.18976036261261697 1213 62-63 -0.129617417893094 1213 64-65 -0.08481857380829183 1213 66-67 -0.07380755525489491 1213 68-69 -0.03411273115902702 1213 70-71 -0.009505292576157842 1213 72-73 0.07699043608857181 1213 74-75 0.06380972196754442 1213 76-77 0.3460441920685966 1213 78-79 -0.2177512045151886 1213 80-81 -0.15676693389224994 1213 82-83 -0.04910987478335471 1213 84-85 -0.07843952273567822 1213 86-87 -0.035648193650807514 1213 88-89 -0.057851306628926125 1213 90-91 -0.008661197853037095 1213 92-93 0.04798800905066258 1213 94-95 0.08966203429947939 1213 96-97 0.16066984124734063 1213 98-99 0.19799648026153704 1213 100-101 0.8337144881615295 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 46.0 8 430.0 9 1261.0 10 799.0 11 334.0 12 460.0 13 704.0 14 1451.0 15 2990.0 16 5387.0 17 8008.0 18 11281.0 19 14877.0 20 19137.0 21 24485.0 22 31187.0 23 39579.0 24 50184.0 25 63656.0 26 81854.0 27 104550.0 28 133678.0 29 172294.0 30 224318.0 31 297421.0 32 402863.0 33 576502.0 34 906948.0 35 1705869.0 36 3771188.0 37 5361685.0 38 1006871.0 39 38.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 18.14482247708354 20.02566355497951 33.862503645467065 27.96701032246989 2 29.75949081690818 21.70669583567457 30.414222500799976 18.11959084661727 3 19.717864137916756 19.313872402143556 41.71614576347209 19.2521176964676 4 19.54422987263427 20.226148416517884 40.22945166135675 20.000170049491096 5 29.29704612537983 19.29111296065319 33.34789387297063 18.06394704099635 6 31.82105420747784 20.22490242363755 30.767123650601103 17.186919718283512 7 28.937754491861018 29.494844909949762 23.33586312169671 18.231537476492505 8 26.672727419175857 21.42375051232042 32.16629343357162 19.7372286349321 9 22.356208624530257 20.52524850161463 36.26803872127219 20.850504152582914 10-11 28.428140443467463 21.70072691273955 28.271109824277413 21.600022819515573 12-13 26.9809018397071 26.211484271721503 24.508883005353958 22.298730883217438 14-15 26.26628266960061 23.96265640958527 26.646679541272437 23.124381379541678 16-17 25.67403555020027 23.767966956134547 26.060243832275205 24.49775366138998 18-19 25.440492381445047 24.479979380039204 26.894836668888466 23.184691569627283 20-21 24.16966123158806 25.094556667981287 27.51942116657441 23.216360933856247 22-23 24.349042050218195 25.090251872479012 26.62059039101595 23.940115686286845 24-25 25.111895040032717 24.941681843510764 26.1393982840353 23.807024832421213 26-27 24.64369033131088 25.109330605659867 26.565922614114484 23.68105644891477 28-29 24.601567121006855 25.593821249471745 25.848223707743585 23.956387921777818 30-31 24.872787945492657 25.293651091893278 25.847200812229293 23.986360150384776 32-33 24.611018695559874 25.768375082536604 25.88555891866948 23.735047303234037 34-35 24.44390383549843 25.788171729960574 25.76853406746682 23.999390367074177 36-37 24.74576336631129 25.604242753415875 25.980323870540857 23.66967000973198 38-39 24.6955698717752 25.34889099823175 25.92698217550539 24.028556954487662 40-41 24.328001588245492 25.80353664242666 25.520876746663923 24.347585022663925 42-43 24.239174594731253 25.694235820899003 25.651631402375745 24.414958181994 44-45 24.46718919741551 25.38268096434072 26.077551571473478 24.07257826677029 46-47 23.947983150846106 25.533133988430258 26.159161627562007 24.359721233161633 48-49 24.088717288551628 25.11737833909629 25.837758652871067 24.956145719481007 50-51 24.096872281961733 25.362992096417425 26.436555627341207 24.103579994279638 52-53 24.210771748029263 25.629146703009187 26.252102365505113 23.907979183456433 54-55 24.213311759507324 25.429386172961436 25.73296284890087 24.624339218630368 56-57 24.116109500167827 26.138117614048472 25.979143269073973 23.76662961670973 58-59 24.175450981000605 26.12070821704142 25.973701543991247 23.73013925796673 60-61 24.758506119693667 25.678881175366815 25.53422336911007 24.028389335829452 62-63 24.215819037000845 26.09584947719938 25.993089507724786 23.69524197807499 64-65 24.615703123540964 25.681045302284716 25.75431651853797 23.948935055636355 66-67 24.722151430221288 25.894193970230518 25.89076488153747 23.492889718010723 68-69 24.477015838617845 25.444145992081758 26.411152955703866 23.66768521359654 70-71 24.29511407273451 26.206925552199973 25.77012442642676 23.727835948638763 72-73 24.20751087257516 25.726027254046034 26.11436716153846 23.952094711840342 74-75 24.371718212333075 25.77694041423194 25.956364837806888 23.8949765356281 76-77 24.1543610666463 25.531293973592444 26.163832818018 24.150512141743253 78-79 24.563043523057228 25.498773790444453 25.555289718784902 24.382892967713417 80-81 24.221139663185923 25.299670743138975 26.242002827565443 24.237186766109666 82-83 24.086520405100547 26.136492206267203 26.009912847746637 23.767074540885613 84-85 24.183837716646075 26.010814731355588 25.646636374340293 24.158711177658045 86-87 23.94414440299557 26.023568980410456 25.88276637124565 24.14952024534832 88-89 23.964305285425212 27.387117329528245 25.061121405842275 23.58745597920427 90-91 23.876341101203803 26.312236974754438 25.346495608235465 24.464926315806295 92-93 23.72662369318456 26.782988509742246 25.294823862424398 24.1955639346488 94-95 23.709388219564946 26.936604822908173 24.946185698156516 24.40782125937037 96-97 23.786457345404383 26.413824321912198 25.079013787557226 24.720704545126193 98-99 24.15705967928253 26.166810369221245 24.996011837983414 24.680118113512812 100-101 24.505693939166044 27.077445697391344 24.257175242582868 24.159685120859745 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4982.0 1 3361.0 2 3210.0 3 3917.0 4 3298.5 5 2754.5 6 1867.5 7 1738.0 8 1860.0 9 1887.0 10 1816.5 11 1740.0 12 1677.5 13 1588.0 14 1603.0 15 1674.5 16 1739.0 17 1924.5 18 2091.5 19 2241.5 20 2469.0 21 2732.5 22 3147.5 23 3688.0 24 4373.0 25 5433.0 26 6484.5 27 7603.5 28 9013.0 29 10606.0 30 12828.0 31 16514.5 32 25712.0 33 40189.0 34 57850.5 35 89771.5 36 128136.0 37 183073.5 38 256846.5 39 308485.5 40 356342.5 41 420213.5 42 500639.0 43 582251.5 44 652098.5 45 758642.0 46 879799.5 47 942154.0 48 975969.5 49 985467.0 50 941530.5 51 862258.0 52 766375.5 53 687024.5 54 623394.0 55 574363.0 56 538887.0 57 450154.5 58 336352.5 59 262045.0 60 203213.5 61 153593.0 62 114341.5 63 78364.5 64 47358.0 65 29000.0 66 18991.5 67 13337.0 68 10766.0 69 8419.0 70 6351.5 71 4775.5 72 3953.5 73 3343.5 74 2655.5 75 2105.0 76 1510.0 77 1101.0 78 876.5 79 702.5 80 557.5 81 378.0 82 244.5 83 183.5 84 130.5 85 93.5 86 83.0 87 95.5 88 86.5 89 68.0 90 57.0 91 49.5 92 39.0 93 29.0 94 28.5 95 22.0 96 14.5 97 10.5 98 7.0 99 2.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11583417413682039 2 0.0 3 0.0 4 0.5692123668907175 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.156010413026947E-4 12-13 0.0 14-15 0.0 16-17 1.6641884681458017E-5 18-19 0.0 20-21 0.0017340843838079256 22-23 4.8261465576228255E-4 24-25 0.0 26-27 7.322429259841528E-5 28-29 5.092416712526154E-4 30-31 0.003132002697050399 32-33 0.0014245453287328063 34-35 0.0012148575817464353 36-37 0.008271016686684635 38-39 0.015896328247728696 40-41 0.013240283452568 42-43 0.010614194049833924 44-45 0.008913393435388915 46-47 0.016462152326898273 48-49 0.016132643010205405 50-51 0.015397071707284958 52-53 0.017630412631536623 54-55 0.01932122811517276 56-57 0.02580157801013251 58-59 0.03322718695499908 60-61 0.01832937178815786 62-63 0.009386022960342322 64-65 0.018615612204678937 66-67 0.025092633722702398 68-69 0.032385107590117305 70-71 0.031602939010088774 72-73 0.033819638049658984 74-75 0.026866658629745827 76-77 0.03432555134397531 78-79 0.017823458493841537 80-81 0.0062174081169927165 82-83 0.008823527258109042 84-85 0.015719924270105243 86-87 0.029985347819051055 88-89 0.02574166722527926 90-91 0.03647901122175598 92-93 0.026663627636632034 94-95 0.03761398775703141 96-97 0.016575317142732186 98-99 0.01921804843014772 100-101 0.2887932816312136 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5022337E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.089091777423437 #Duplication Level Percentage of deduplicated Percentage of total 1 76.4045163489946 16.877063738425914 2 10.04818881727734 4.439107299634381 3 4.213366276436873 2.7920830311634477 4 1.8788034943626426 1.660042512748811 5 1.2088852878872363 1.3351589036259055 6 0.8891492596854069 1.1784299760611434 7 0.5590591789435212 0.8644376658886103 8 0.39399912894200023 0.6962466335539785 9 0.36727625860569607 0.7301519085608932 >10 3.0150316944956965 13.73896952073442 >50 0.43232215474918256 6.6381734623654545 >100 0.4766145526687113 22.607884046426822 >500 0.07513773281607528 11.6609802987855 >1k 0.036881851749286765 13.485662167369982 >5k 6.477715866894253E-4 0.9305738193284627 >10k+ 1.2019079913546554E-4 0.3650350153262353 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTTTGAGTAGCGAAAGACAGGTGAGAATCCTGTCCACCGTAAGACTAAG 15810 0.1052432787255405 No Hit CTTTGTGTTTGAGGGGTCCATCCCGGATTACCAATCTCAGATAAACTCCG 15160 0.10091638870836142 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.1301612392266264E-4 0.0 0.0 2 2.1301612392266264E-4 0.0 0.0 3 2.1967287779524583E-4 0.0 0.0 4 2.2632963166782904E-4 0.0 0.0 5 2.2632963166782904E-4 0.0 0.0 6 2.3964313941299547E-4 0.0 0.0 7 2.4629989328557865E-4 0.0 0.0 8 2.4629989328557865E-4 0.0 0.0 9 2.8624041652107793E-4 0.0 0.0 10-11 3.627930860557848E-4 0.0 0.0 12-13 4.7928627882599093E-4 0.0 0.0 14-15 6.090929793413635E-4 0.0 0.0 16-17 7.72183449219652E-4 6.6567538725832075E-6 0.0 18-19 9.851995731423146E-4 6.6567538725832075E-6 0.0 20-21 0.0012281710894916018 6.6567538725832075E-6 0.0 22-23 0.0014644858519683055 6.6567538725832075E-6 0.0 24-25 0.0018106370533426323 9.985130808874812E-6 0.0 26-27 0.0021601166316532507 1.3313507745166415E-5 0.0 28-29 0.0025262380946453273 1.3313507745166415E-5 0.0 30-31 0.0030621067813882755 1.3313507745166415E-5 0.0 32-33 0.0036578862529844723 1.3313507745166415E-5 0.0 34-35 0.004313576509433918 1.3313507745166415E-5 0.0 36-37 0.005262163936277025 1.3313507745166415E-5 0.0 38-39 0.006373841832998421 1.3313507745166415E-5 0.0 40-41 0.007738476376877978 1.3313507745166415E-5 0.0 42-43 0.009642307984436774 1.3313507745166415E-5 0.0 44-45 0.012012112363076397 1.3313507745166415E-5 0.0 46-47 0.01500432322880255 1.3313507745166415E-5 0.0 48-49 0.01892515125975406 1.3313507745166415E-5 0.0 50-51 0.024223927342330292 1.3313507745166415E-5 0.0 52-53 0.030973875769129664 1.3313507745166415E-5 0.0 54-55 0.03965095444204188 1.3313507745166415E-5 0.0 56-57 0.05000553509084505 1.3313507745166415E-5 0.0 58-59 0.06321586315098643 1.3313507745166415E-5 0.0 60-61 0.0796380749546492 1.3313507745166415E-5 0.0 62-63 0.0997747554192134 1.3313507745166415E-5 0.0 64-65 0.12487737427272468 1.3313507745166415E-5 0.0 66-67 0.15620072962016496 1.3313507745166415E-5 0.0 68-69 0.1942474063789143 1.3313507745166415E-5 0.0 70-71 0.2420096154146988 1.3313507745166415E-5 0.0 72-73 0.3002795104383559 1.3313507745166415E-5 0.0 74-75 0.3713237161435002 1.3313507745166415E-5 0.0 76-77 0.45889997009120487 1.3313507745166415E-5 0.0 78-79 0.5670855340284271 1.3313507745166415E-5 0.0 80-81 0.6977709260549807 1.3313507745166415E-5 0.0 82-83 0.854091477244852 1.3313507745166415E-5 0.0 84-85 1.0433097060730299 1.3313507745166415E-5 0.0 86-87 1.2650361924379676 1.3313507745166415E-5 0.0 88-89 1.5269861140779892 1.3313507745166415E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 148265 0.0 78.517044 1 TTTGTGT 158020 0.0 73.76291 2 TTGTGTT 179095 0.0 67.127785 3 TGTGTTT 186850 0.0 64.66925 4 GTGTTTG 203290 0.0 59.532063 5 ACTTTGT 22945 0.0 48.939873 1 TTTGAGT 74815 0.0 41.861374 3 GTTTGAA 26350 0.0 41.189198 2 TGTTTGA 350310 0.0 40.91574 1 GTTTGAG 196050 0.0 40.24265 2 TTGAGGG 41080 0.0 39.254913 9 TTGAGTC 27585 0.0 39.068436 4 TTTGAGG 76135 0.0 38.626545 8 TTTGATT 35800 0.0 37.518997 3 TGAGTCT 5105 0.0 37.288727 5 TGAGTGG 11285 0.0 34.53588 5 TTGATTC 10415 0.0 34.52257 4 GTTTGAT 96705 0.0 34.41011 2 TTGAGGC 12505 0.0 34.355305 9 TTGAGTG 32960 0.0 34.247406 4 >>END_MODULE