FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l03n01_30076.34100000035ce6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l03n01_30076.34100000035ce6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16090946
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT189170.11756300717185926No Hit
CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC185650.11537544156819618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT370300.057.716561
CTTTGTG1069700.055.8729671
TTGTGTT1208250.051.0279123
TTTGTGT1186200.050.515342
TGTGTTT1294850.047.8588184
GTGTTTG1447350.042.9582025
TTCCGAT11000.041.4279256
TCCGATC12000.038.7667587
TTGAGGG399150.031.7295829
TTTGAGG593000.030.8751878
GTTTGAG1229700.030.3397312
TGTTTGA2121450.030.1287121
GTTTGAA127750.029.7501832
CTTGTGT60500.029.2789842
GTTTGAC359300.027.3745637
TTTGAGT414150.027.2999293
TTGAGGC59450.026.905014
TTTGAGA199000.026.296323
AGTCCTG37200.026.1592677
TTTGACC116000.025.985468
Produced by FastQC (version 0.11.2)