FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l02n02_7637.34200000035bca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l02n02_7637.34200000035bca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16365827
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA327410.20005710679943028No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247820.15142528391629703No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG5500.00869043135.4350494-95
CTTGCGA20907.232951E-571.281694-95
CTCTATA16650.002627037567.1074594-95
TGGGACG18200.003738115361.39225894-95
CTTTGTG1699400.059.4760861
AGATCGA19000.00443238358.8073294-95
GCTACGA20600.006103841654.2397694-95
TTTGTGT1871350.054.110552
TTGTGTT2000600.051.741663
ACTTTGT563950.051.5623631
TGTGTTT2160700.048.078434
TAACTTT288900.044.6023751
GTGTTTG2344650.044.276895
AACTTTG295550.043.7718242
TATGGGA44105.6464094E-542.22747894-95
GATCGGT20100.037.0593494-95
TCAAATA41650.002170505235.7691694-95
GAGATCA41800.00220913435.640894-95
TCCGATC11455.456968E-1232.5280794-95
TTTGAGG792650.031.3126288
Produced by FastQC (version 0.11.2)