FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l02n02_6667.34200000035d92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l02n02_6667.34200000035d92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16958577
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA12801.9281288E-10172.4708194-95
CTAGTCA13157.710209E-6111.9202394-95
GAGATCA16152.1397129E-591.1300894-95
GGTACGA12258.1563584E-490.1071994-95
AACCGAG16902.6803427E-587.0858594-95
AGTGTCG12959.094947E-1285.2365594-95
CCTACGA13750.001290474180.2773294-95
CAGATCT14200.001466451977.7333294-95
GGACATA14250.001487054577.4605794-95
CGTCGTA24051.6723407E-676.4943394-95
CACTCAG14550.00161523975.8634594-95
CGCTCGA14600.001637378475.60364594-95
CTTTGTG1751650.072.5455551
TTTGTGT1903750.066.8115162
TTGTGTT2089500.062.1448553
TCTGAGC18650.004321533759.18569694-95
TGTGTTT2209500.058.967854
AGTGCGA12650.058.1719794-95
AGGTCTG19050.004700302657.9429594-95
AGACATC26402.4403716E-455.7481494-95
Produced by FastQC (version 0.11.2)