##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l02n02_6628.34200000035d5f.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16300938 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.40700461531723 33.0 33.0 33.0 33.0 33.0 2 32.40563432607375 33.0 33.0 33.0 33.0 33.0 3 32.390715920764805 33.0 33.0 33.0 33.0 33.0 4 36.232206269357015 37.0 37.0 37.0 37.0 37.0 5 36.229299258729775 37.0 37.0 37.0 37.0 37.0 6 36.21145948779144 37.0 37.0 37.0 37.0 37.0 7 36.13545760372808 37.0 37.0 37.0 37.0 37.0 8 36.209664437715176 37.0 37.0 37.0 37.0 37.0 9 36.16348574542152 37.0 37.0 37.0 37.0 37.0 10-11 36.15451929821462 37.0 37.0 37.0 37.0 37.0 12-13 36.13959871511689 37.0 37.0 37.0 37.0 37.0 14-15 38.13691212125339 40.0 37.0 40.0 37.0 40.0 16-17 38.10138803055382 40.0 37.0 40.0 37.0 40.0 18-19 38.041893018671686 40.0 37.0 40.0 37.0 40.0 20-21 37.99281952363723 40.0 37.0 40.0 37.0 40.0 22-23 37.94832131746038 40.0 37.0 40.0 37.0 40.0 24-25 37.892565231522255 40.0 37.0 40.0 37.0 40.0 26-27 37.827309876278285 40.0 37.0 40.0 33.0 40.0 28-29 37.76083551142885 40.0 37.0 40.0 33.0 40.0 30-31 37.69202726248023 40.0 37.0 40.0 33.0 40.0 32-33 37.64595693818356 40.0 37.0 40.0 33.0 40.0 34-35 37.56269071755257 40.0 37.0 40.0 33.0 40.0 36-37 37.45693217163331 40.0 37.0 40.0 33.0 40.0 38-39 37.30923904501692 40.0 37.0 40.0 33.0 40.0 40-41 37.21582810142582 40.0 37.0 40.0 33.0 40.0 42-43 37.09990909725563 40.0 37.0 40.0 33.0 40.0 44-45 36.91000106865016 38.5 37.0 40.0 33.0 40.0 46-47 36.725467761425755 37.0 37.0 40.0 33.0 40.0 48-49 36.535511484063065 37.0 37.0 40.0 33.0 40.0 50-51 35.156941336750066 37.0 35.0 38.5 30.0 38.5 52-53 35.535070926593306 37.0 35.0 38.5 33.0 40.0 54-55 36.14790796087931 37.0 37.0 40.0 33.0 40.0 56-57 36.04023624284689 37.0 37.0 40.0 33.0 40.0 58-59 35.81936346239708 37.0 37.0 38.5 33.0 40.0 60-61 35.582485713398825 37.0 37.0 37.0 33.0 40.0 62-63 35.32755173352601 37.0 33.0 37.0 33.0 40.0 64-65 35.10079140844533 37.0 33.0 37.0 33.0 40.0 66-67 34.84323132202576 37.0 33.0 37.0 30.0 38.5 68-69 34.63878127749459 37.0 33.0 37.0 27.0 37.0 70-71 34.45405706101084 37.0 33.0 37.0 27.0 37.0 72-73 34.27239579710076 37.0 33.0 37.0 27.0 37.0 74-75 34.0142687801156 37.0 33.0 37.0 27.0 37.0 76-77 33.942618302087894 37.0 33.0 37.0 27.0 37.0 78-79 33.829560176230345 37.0 33.0 37.0 27.0 37.0 80-81 33.69605617173687 37.0 33.0 37.0 27.0 37.0 82-83 33.58647848976543 37.0 33.0 37.0 27.0 37.0 84-85 33.47626280769855 37.0 33.0 37.0 27.0 37.0 86-87 33.383912263208416 37.0 33.0 37.0 27.0 37.0 88-89 33.291429763121606 37.0 33.0 37.0 27.0 37.0 90-91 33.20460699868928 37.0 33.0 37.0 27.0 37.0 92-93 33.09015840070062 37.0 33.0 37.0 27.0 37.0 94-95 32.94999536223008 37.0 33.0 37.0 27.0 37.0 96-97 32.868526768214195 35.0 33.0 37.0 27.0 37.0 98-99 31.37199224363653 33.0 30.0 37.0 21.0 37.0 100-101 2.7175422972592127 2.0 2.0 2.0 2.0 4.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1828941602748344 2306 2 0.14902052990287018 2306 3 0.17035295697446884 2306 4 0.20939939253107553 2306 5 0.21809262243203165 2306 6 0.2370409469550694 2306 7 0.28919579267770956 2306 8 0.21642437161335693 2306 9 0.21511751485437003 2306 10-11 0.2335846554370633 2306 12-13 0.1841376930851908 2306 14-15 0.2900766442036158 2306 16-17 0.2931831231755666 2306 18-19 0.3143724826137557 2306 20-21 0.3436256810435694 2306 22-23 0.36787544639420133 2306 24-25 0.2905193013011882 2306 26-27 0.21522963186403388 2306 28-29 0.16545993659833869 2306 30-31 0.18454867640856776 2306 32-33 0.2253618966252091 2306 34-35 0.1622723829168109 2306 36-37 0.19574659056832644 2306 38-39 0.2521122613570199 2306 40-41 0.20130310684674413 2306 42-43 0.1477200756509589 2306 44-45 0.14000884507603217 2306 46-47 0.10440929991027303 2306 48-49 0.10754660685553574 2306 50-51 0.049089826862839914 2306 52-53 0.11082382450393169 2306 54-55 0.30992749638084405 2306 56-57 0.2851580129108058 2306 58-59 0.30188040368464897 2306 60-61 0.22802030392324468 2306 62-63 0.19203737535159604 2306 64-65 0.23583338015763644 2306 66-67 0.15612223106375467 2306 68-69 0.1791141883806162 2306 70-71 0.20373901881559675 2306 72-73 0.20867841353521044 2306 74-75 0.12136025497764535 2306 76-77 0.03221123208394516 2306 78-79 0.13245305900861126 2306 80-81 0.11227425371924227 2306 82-83 0.06307847791455856 2306 84-85 0.056058485951076875 2306 86-87 0.017627904960917817 2306 88-89 -0.11246202928533933 2306 90-91 -0.19705322973609185 2306 92-93 -0.21537180083848284 2306 94-95 -0.28671049408257687 2306 96-97 -0.21973954024856113 2306 98-99 -0.29392207167393636 2306 100-101 2.3144436156669337 2307 1 0.08634472984385155 2307 2 0.07074666251008921 2307 3 0.0531217824043253 2307 4 0.06037762070211983 2307 5 0.09529943823802967 2307 6 0.12117453454914795 2307 7 0.20354023851367486 2307 8 0.15226316307436605 2307 9 0.16338554216888923 2307 10-11 0.11641590127595691 2307 12-13 0.07352011234083733 2307 14-15 0.12611817288391336 2307 16-17 0.11011053621026434 2307 18-19 0.10009518261491479 2307 20-21 0.01570972824791994 2307 22-23 0.027640405838575077 2307 24-25 -0.018543708280063242 2307 26-27 0.06247336023540839 2307 28-29 -0.006067069567528449 2307 30-31 -0.00657275203067087 2307 32-33 -0.03239930915943745 2307 34-35 -0.0571851568738353 2307 36-37 -0.054349781482102344 2307 38-39 -0.08768721350934072 2307 40-41 -0.08565998790411555 2307 42-43 -0.09085189825675855 2307 44-45 -0.018532744931533784 2307 46-47 -0.04762269011898468 2307 48-49 -0.025346375109080554 2307 50-51 -0.1958806779445723 2307 52-53 -0.0778854665570563 2307 54-55 0.08366122582383184 2307 56-57 0.09632846874246326 2307 58-59 0.08657733288800529 2307 60-61 0.14864590240215136 2307 62-63 0.13516654695133212 2307 64-65 0.09396067320604828 2307 66-67 0.12406955760331329 2307 68-69 0.11171496846358764 2307 70-71 0.11500659123235124 2307 72-73 0.04763815658898096 2307 74-75 -0.015203850963175114 2307 76-77 0.04728755119064232 2307 78-79 0.03924449763464111 2307 80-81 -0.08587874083790581 2307 82-83 -0.020112709549373164 2307 84-85 -0.0383159306328551 2307 86-87 -0.012643858798661256 2307 88-89 -0.007472398974776695 2307 90-91 -0.07739353802509186 2307 92-93 -0.2317557466053728 2307 94-95 -0.2256890613827025 2307 96-97 -0.1350055736734319 2307 98-99 0.7922172148380611 2307 100-101 4.388217420871705 2201 1 0.11123076595129078 2201 2 0.09376906710617305 2201 3 0.11680053879720731 2201 4 0.13886874480296285 2201 5 0.13304707436875418 2201 6 0.1174604697291457 2201 7 0.10122091317474258 2201 8 0.11869068779673597 2201 9 0.1673774701308588 2201 10-11 0.14863694367518576 2201 12-13 0.14365897651904191 2201 14-15 0.16275193569797608 2201 16-17 0.1969979951141383 2201 18-19 0.1352397320483263 2201 20-21 0.21478672207690153 2201 22-23 0.20146450959543216 2201 24-25 0.2360024067146682 2201 26-27 0.22750670348895596 2201 28-29 0.1783695025012335 2201 30-31 0.17241978246634915 2201 32-33 0.19971254884342926 2201 34-35 0.16482709998648204 2201 36-37 0.1809176762141007 2201 38-39 0.14082618255859103 2201 40-41 0.06461874595261463 2201 42-43 0.0813637284583848 2201 44-45 0.07950672351731924 2201 46-47 0.14600601500146837 2201 48-49 0.08307378708648372 2201 50-51 0.06742666873145708 2201 52-53 0.14845228828202295 2201 54-55 0.2849886264356485 2201 56-57 0.14342586425399162 2201 58-59 0.1019137064445843 2201 60-61 0.09370154188418667 2201 62-63 0.14898442631071163 2201 64-65 0.08959783153811429 2201 66-67 0.062061117870257476 2201 68-69 0.027110011481745744 2201 70-71 0.07772620274799635 2201 72-73 0.029145959595020088 2201 74-75 -0.06306866427438251 2201 76-77 -0.0118428851156267 2201 78-79 -0.0737289824963483 2201 80-81 -0.15093079771956752 2201 82-83 -0.19023612999730943 2201 84-85 -0.24381675875537923 2201 86-87 -0.1669300640889375 2201 88-89 -0.17974828831690104 2201 90-91 -0.20145834454310574 2201 92-93 -0.28739317181286594 2201 94-95 -0.37323292060618485 2201 96-97 -0.3624746943000474 2201 98-99 -0.4044122537078394 2201 100-101 2.136558827860498 1101 1 0.05776931084943726 1101 2 0.07831649491232895 1101 3 0.07666926843784694 1101 4 0.04849680133526135 1101 5 0.10092792985813759 1101 6 0.10730581946534556 1101 7 0.14549554577624235 1101 8 0.10076874966654259 1101 9 0.09121142671265403 1101 10-11 0.10297316384579602 1101 12-13 0.08458854809502725 1101 14-15 0.017856999699553455 1101 16-17 0.09996621591231047 1101 18-19 0.052581216134463205 1101 20-21 0.0929955755477394 1101 22-23 0.06124143004714 1101 24-25 0.11561597514638322 1101 26-27 0.1401339747605448 1101 28-29 0.07306933639150515 1101 30-31 0.08109950122817366 1101 32-33 0.035331137767933285 1101 34-35 -0.012799682013501013 1101 36-37 0.08935831907655967 1101 38-39 0.07825319617408155 1101 40-41 0.0588325633063107 1101 42-43 -0.0039221962083431094 1101 44-45 0.05244490869974783 1101 46-47 0.10645552217152243 1101 48-49 -0.04238548125433539 1101 50-51 -0.109176767330041 1101 52-53 0.10142784815430872 1101 54-55 0.20598897256498816 1101 56-57 0.16367250971080693 1101 58-59 0.19283897162171826 1101 60-61 0.2131640588267345 1101 62-63 0.19431459164407983 1101 64-65 0.170222311939753 1101 66-67 0.1585877140278953 1101 68-69 0.12391003364049169 1101 70-71 0.11494141432359584 1101 72-73 0.10099476738972868 1101 74-75 -0.06371874854232118 1101 76-77 0.054740996255823404 1101 78-79 0.00288043804432192 1101 80-81 -0.11255471849153054 1101 82-83 -0.06980079148605256 1101 84-85 -0.08381174068872355 1101 86-87 -0.15679888673162168 1101 88-89 -0.04057295763399793 1101 90-91 -0.20962351940311663 1101 92-93 -0.29150202221059374 1101 94-95 -0.3294274226489975 1101 96-97 -0.19994738000490742 1101 98-99 -0.25364870694964736 1101 100-101 2.1207220763651873 2304 1 0.10096242197040795 2304 2 0.08300639032061241 2304 3 0.10842548818093434 2304 4 0.11924486927228628 2304 5 0.0839943567635757 2304 6 0.11148907565267052 2304 7 0.1621081021663926 2304 8 0.14143335714710759 2304 9 0.13303619151116663 2304 10-11 0.12250268172208933 2304 12-13 0.08851123742127243 2304 14-15 0.15182238192021913 2304 16-17 0.1335884201988904 2304 18-19 0.1632166440701539 2304 20-21 0.13360045127421216 2304 22-23 0.15801068014771857 2304 24-25 0.16782277717216232 2304 26-27 0.19419406323672206 2304 28-29 0.15797717876034056 2304 30-31 0.10493047088660035 2304 32-33 0.14287314385619965 2304 34-35 0.20032127322437532 2304 36-37 0.13961062435634375 2304 38-39 0.12035351291068963 2304 40-41 0.10116659794978489 2304 42-43 0.14246986981074627 2304 44-45 0.1809750828574863 2304 46-47 0.18252167852556767 2304 48-49 0.16225209554566078 2304 50-51 -0.007695617006994837 2304 52-53 0.06733046074786131 2304 54-55 0.19390150654506755 2304 56-57 0.25445409938124186 2304 58-59 0.22376603290283725 2304 60-61 0.20062951233493465 2304 62-63 0.20493145353622566 2304 64-65 0.2687827633946469 2304 66-67 0.18220021628881256 2304 68-69 0.1216446706067984 2304 70-71 0.21940527514301778 2304 72-73 0.22592512458201242 2304 74-75 0.02621078201785565 2304 76-77 0.07794848906924301 2304 78-79 -0.059889449757427826 2304 80-81 0.06815876930065912 2304 82-83 0.09081289469784792 2304 84-85 -0.026725107462731046 2304 86-87 -0.01406812318139572 2304 88-89 0.08092058648294653 2304 90-91 -0.023479878611929905 2304 92-93 0.004532857209184726 2304 94-95 -0.0038108091256745524 2304 96-97 -0.08524864522603082 2304 98-99 0.028147356082193653 2304 100-101 2.123904903879295 2202 1 0.15493315006244757 2202 2 0.13750916555225245 2202 3 0.1408536406187082 2202 4 0.15089868829689124 2202 5 0.12469658766701741 2202 6 0.15360904808743925 2202 7 0.22465927702694444 2202 8 0.17088713766113273 2202 9 0.19412945129512593 2202 10-11 0.15331412575338987 2202 12-13 0.15080410994736582 2202 14-15 0.17147207883227367 2202 16-17 0.14426390734870154 2202 18-19 0.16443717062194452 2202 20-21 0.21040470421868207 2202 22-23 0.20338578800991058 2202 24-25 0.23742334430612289 2202 26-27 0.22218339908353357 2202 28-29 0.16170832734645302 2202 30-31 0.1395934818718132 2202 32-33 0.17296703990284357 2202 34-35 0.23867121299407046 2202 36-37 0.2017701994140566 2202 38-39 0.2457390650053668 2202 40-41 0.25092535325374143 2202 42-43 0.22088000631431726 2202 44-45 0.17456011912561564 2202 46-47 0.267985622613061 2202 48-49 0.08507784358230452 2202 50-51 0.139977375403447 2202 52-53 0.25682135477020296 2202 54-55 0.3493406899891909 2202 56-57 0.3192482942216941 2202 58-59 0.2823153223857773 2202 60-61 0.30730025282586126 2202 62-63 0.2749543186641148 2202 64-65 0.3420419755365103 2202 66-67 0.32343107946934424 2202 68-69 0.2684877980703888 2202 70-71 0.3380128231585715 2202 72-73 0.3263300681379562 2202 74-75 0.15618197645204646 2202 76-77 0.14281292202898754 2202 78-79 0.20227477404865368 2202 80-81 0.12378297115158432 2202 82-83 0.14511911969206892 2202 84-85 0.11756755790393214 2202 86-87 0.13277354362811167 2202 88-89 0.11987738244728519 2202 90-91 0.08705474101295607 2202 92-93 0.11416848093038112 2202 94-95 0.07970090519777528 2202 96-97 -0.034874823384157594 2202 98-99 0.043491298210589946 2202 100-101 2.1770723189144143 1102 1 -0.017953203885220148 1102 2 -0.036134099301776246 1102 3 0.001983060561933314 1102 4 -0.12864989974223562 1102 5 -0.10747860695508393 1102 6 -0.022022247821830376 1102 7 0.06758436180913918 1102 8 -0.008731302485323056 1102 9 0.0698225996855868 1102 10-11 0.026565570076385825 1102 12-13 -0.011460961466035258 1102 14-15 -0.048489380820171846 1102 16-17 -0.002015669419840549 1102 18-19 0.023240137309748832 1102 20-21 0.021688852467789843 1102 22-23 0.04539806834507232 1102 24-25 0.06342304655144204 1102 26-27 0.1205999173032879 1102 28-29 0.06882640790227867 1102 30-31 -0.02023911024960512 1102 32-33 -0.017352044771335784 1102 34-35 0.0037052727659059315 1102 36-37 0.026008353820976993 1102 38-39 0.030472222724320375 1102 40-41 0.07587099112495821 1102 42-43 0.10478990912010033 1102 44-45 0.13108221927043928 1102 46-47 0.18425697579899492 1102 48-49 0.12065188555579454 1102 50-51 0.05039239485027025 1102 52-53 0.11978935200966845 1102 54-55 0.18200944005710795 1102 56-57 0.21039901564871855 1102 58-59 0.2262046678878491 1102 60-61 0.1740665952748941 1102 62-63 0.16435256207679316 1102 64-65 0.17737570642702138 1102 66-67 0.15132913927489255 1102 68-69 0.15090398349577328 1102 70-71 0.13834891488916412 1102 72-73 0.23512110563120814 1102 74-75 0.13104711601928898 1102 76-77 0.10750655143002774 1102 78-79 0.10989707484112898 1102 80-81 0.09451557658230314 1102 82-83 0.12930495381692708 1102 84-85 0.06361830445788286 1102 86-87 0.05702872616482324 1102 88-89 0.07124266368145982 1102 90-91 0.01201780368965899 1102 92-93 -0.033081174467952223 1102 94-95 0.03283851062622034 1102 96-97 -0.061836511662278326 1102 98-99 0.16947782545181767 1102 100-101 2.381811729646913 2305 1 0.02997279152932464 2305 2 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86-87 -0.3642784375326542 1213 88-89 -0.36869801779285183 1213 90-91 -0.2642511312600888 1213 92-93 -0.40626269583579244 1213 94-95 -0.2615995857497708 1213 96-97 -0.34074803183816016 1213 98-99 0.0011273288415054594 1213 100-101 -0.7330488733554836 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 167.0 3 141.0 4 168.0 5 314.0 6 2078.0 7 18164.0 8 31920.0 9 28908.0 10 22147.0 11 16397.0 12 14250.0 13 14213.0 14 14808.0 15 16353.0 16 18742.0 17 21702.0 18 25073.0 19 28951.0 20 33738.0 21 39388.0 22 45955.0 23 54004.0 24 64946.0 25 79575.0 26 100424.0 27 133344.0 28 157483.0 29 194342.0 30 257589.0 31 353446.0 32 501861.0 33 764516.0 34 1331153.0 35 2879730.0 36 6186403.0 37 2840026.0 38 8519.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.28332512222927 21.260656154858086 31.623239242814506 26.83277948009813 2 28.144978412497736 20.79229662179371 31.028418174620953 20.0343067910876 3 21.457295379127643 19.205319237109414 39.77423325689693 19.563152126866015 4 21.006426143045193 18.989730100028495 39.231718652701836 20.772125104224475 5 29.384342549209325 18.36910702984418 33.17445958676175 19.072090834184742 6 31.113257814003646 18.98184890056171 31.48182357601615 18.423069709418492 7 29.856590898081137 26.246893185505826 24.908791766973057 18.98772414943998 8 27.824255298615412 20.879209728257525 31.05809988877073 20.238435084356333 9 23.02978962120814 19.798476845167947 35.95291001808215 21.21882351554176 10-11 28.786903659069434 20.650125262646245 28.442729991829296 22.12024108645502 12-13 27.67183726928708 25.411760464977178 23.924670267997104 22.991731997738636 14-15 27.341504103544466 22.88678177530734 25.486350446493006 24.285363674655187 16-17 26.83062752920276 22.384041801527545 25.056867004543065 25.728463664726625 18-19 26.747547569260217 23.212452820096143 25.464870153492075 24.57512945715156 20-21 25.350995432180873 24.097214952865244 26.005162329274857 24.54662728567903 22-23 25.560196000504355 23.984478635968365 25.579769518903557 24.87555584462373 24-25 25.856422667556732 24.00405976484329 25.18969116731843 24.949826400281548 26-27 25.610429718569904 24.056791466859657 25.527325602497932 24.805453212072504 28-29 25.438113501766992 24.508860041259826 25.10684899403267 24.946177462940515 30-31 25.623369891656665 24.244695097760587 24.842821976859412 25.289113033723336 32-33 25.3317411718269 24.510156846660408 25.100090209315272 25.058011772197418 34-35 25.302929108938628 24.612703499072968 24.892457951062706 25.191909440925702 36-37 25.541118240894445 24.46152954893518 24.916307418105735 25.081044792064645 38-39 25.417889373075912 24.219889038049136 25.017620860050364 25.344600728824577 40-41 25.480538573255846 24.459653262536584 24.567757625080212 25.492050539127355 42-43 25.459437872596624 24.624688761552076 24.546868975995984 25.36900438985532 44-45 25.543158299299552 24.364117528959117 24.69117058141587 25.40155359032546 46-47 25.113113157272316 24.579440920446288 24.829121017246248 25.478324905035144 48-49 25.16106384945434 24.293636398572975 24.793515624301605 25.75178412767108 50-51 25.18455825439534 24.5615747054415 25.1723735456366 25.08149349452656 52-53 25.200471937922124 24.40198782100838 25.30128772282519 25.09625251824431 54-55 25.14751758938602 24.438501007824613 24.767768366062004 25.64621303672736 56-57 25.148025648368943 24.737838145272132 25.04525990312177 25.068876303237158 58-59 25.07599891484534 24.881157626086566 25.036525062502058 25.006318396566034 60-61 25.553758399759563 24.7794120447843 24.575074058696732 25.091755496759404 62-63 25.178885132240197 25.120999735804013 24.98638324905291 24.713731882902884 64-65 25.453940777825835 24.86063001501381 24.646759896418303 25.03866931074205 66-67 25.89959029842342 24.878547251764278 24.683209372729358 24.53865307708294 68-69 25.5822702077821 24.621488269687518 25.042948744493398 24.753292778036982 70-71 25.34814868380636 24.993761624091857 24.693892578530654 24.964197113571128 72-73 25.22553783268222 24.598942226527974 25.044597642226723 25.13092229856308 74-75 25.109025166688802 24.471924927171926 25.148514863672077 25.270535042467195 76-77 24.937018890672448 24.420721746195433 25.080209367164656 25.562049995967463 78-79 25.605498881114613 24.416467797464517 24.584611338694927 25.393421982725943 80-81 25.220208739816684 24.35520232224154 25.125892909453356 25.298696028488422 82-83 25.269843315275985 24.94925351200774 24.96265052329883 24.818252649417445 84-85 25.30969056723393 24.8814206948252 24.67406611375393 25.134822624186942 86-87 25.054340358569316 24.969478197523884 24.978782921767692 24.997398522139108 88-89 25.002488264863093 25.969608228387404 24.28032244467809 24.747581062071415 90-91 25.100414309846858 25.104557008500407 24.470671831005454 25.32435685064729 92-93 24.89668554711048 25.634265421968077 24.396678780661244 25.0723702502602 94-95 25.081328186529174 25.748493857816655 23.90540666369577 25.2647712919584 96-97 24.943162387905478 25.17404020026325 24.231600506808704 25.651196905022573 98-99 25.469852082552908 25.102354956665884 24.1554653169148 25.27232764386641 100-101 33.16374085482546 40.720053094359976 13.867559648913023 12.248646401901539 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 16426.0 1 9511.5 2 3627.5 3 4056.5 4 3742.5 5 3136.5 6 2153.5 7 2385.5 8 2855.0 9 3078.5 10 3197.5 11 3227.0 12 3178.0 13 3266.0 14 3419.0 15 3540.5 16 3546.0 17 3413.5 18 3257.0 19 3071.5 20 2929.0 21 2935.0 22 3101.0 23 3322.5 24 3716.5 25 4317.5 26 5104.0 27 6018.5 28 7107.0 29 8552.5 30 10708.5 31 13887.0 32 21701.5 33 32929.5 34 44937.5 35 66768.0 36 96952.5 37 134871.5 38 174750.5 39 216798.0 40 262076.0 41 325587.0 42 392478.0 43 456476.5 44 566537.5 45 700316.0 46 814943.5 47 890051.5 48 955592.0 49 965090.5 50 921867.0 51 920099.5 52 934591.0 53 927728.5 54 910095.5 55 893135.5 56 824230.0 57 681373.5 58 539814.0 59 433736.5 60 338139.0 61 252218.0 62 171595.5 63 104940.5 64 61879.5 65 33185.5 66 18149.0 67 13221.0 68 10360.0 69 7811.0 70 6268.0 71 5169.0 72 4496.0 73 3734.0 74 3013.0 75 2420.0 76 1923.5 77 1721.5 78 1564.0 79 1233.0 80 917.0 81 693.0 82 543.5 83 431.5 84 344.5 85 297.0 86 261.5 87 230.5 88 229.0 89 217.5 90 163.5 91 108.5 92 82.0 93 71.5 94 69.5 95 56.0 96 32.5 97 17.0 98 15.0 99 11.0 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.023931138195851062 2 0.041641775461019485 3 0.0790629349059545 4 0.026881888637328724 5 0.03312079341691871 6 0.07602629983624255 7 0.08439391647278212 8 0.07398347260752725 9 0.09365105247317669 10-11 0.10522707343589677 12-13 0.10401855402431444 14-15 0.11753924835491063 16-17 0.09687479334011331 18-19 0.10830358351157461 20-21 0.1091010836308929 22-23 0.11493203642637007 24-25 0.11178191095506283 26-27 0.11257634376622988 28-29 0.1105979300086903 30-31 0.12388857622794468 32-33 0.11196594944413629 34-35 0.13398308735362346 36-37 0.14239057899612895 38-39 0.16938902534320419 40-41 0.1363357127056124 42-43 0.128928163520406 44-45 0.1598404950684433 46-47 0.15527634053942171 48-49 0.15156489767644046 50-51 0.13703505896409152 52-53 0.14858654146160177 54-55 0.16426662073065978 56-57 0.14792707020908857 58-59 0.14155013656269352 60-61 0.1471326373979215 62-63 0.13101700037138966 64-65 0.12430266282835993 66-67 0.1253271437508688 68-69 0.15066617638813176 70-71 0.15131951302434252 72-73 0.1473074739625413 74-75 0.3214293557830844 76-77 0.28014952268390936 78-79 0.30090292963509213 80-81 0.31783447062984965 82-83 0.3131414891584766 84-85 0.29508731338037114 86-87 0.28068630161037356 88-89 0.2737879255782704 90-91 0.26636197254415667 92-93 0.2715058483137596 94-95 0.2857473600598935 96-97 0.25972738501305875 98-99 0.26037458703296706 100-101 88.25171287689089 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6300938E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.682471424934707 #Duplication Level Percentage of deduplicated Percentage of total 1 74.20155973790318 13.86268519488962 2 10.112654621189037 3.778587619811962 3 4.454464824641543 2.4966123539922727 4 1.9802892268867556 1.479867875776714 5 1.4484719947120992 1.3530518325513485 6 0.8771573550953857 0.9832480333044511 7 0.6547252529267956 0.8562320080291606 8 0.6349870171647375 0.9490501442707786 9 0.4750760311053185 0.7988034938216853 >10 3.6172686531632157 14.036475405846224 >50 0.6341598142145927 8.372913212913542 >100 0.7970752090637594 31.538687460937936 >500 0.08539281055767116 10.790792244445404 >1k 0.025934965576754235 7.620120209251812 >5k 6.504400577650866E-4 0.7819222103480272 >10k+ 1.3204574133709998E-4 0.3009506998090697 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTGTGTTTGAGGGGTCCATCCCGGATTACCAATCTCAGATAAACTCCG 16644 0.10210455373795055 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.288269423514156E-4 0.0 0.0 2 1.288269423514156E-4 0.0 0.0 3 1.3496155865386396E-4 0.0 0.0 4 1.3496155865386396E-4 0.0 0.0 5 1.3496155865386396E-4 0.0 0.0 6 1.5336540756120904E-4 0.0 0.0 7 2.085769542832443E-4 0.0 0.0 8 2.085769542832443E-4 0.0 0.0 9 2.453846520979345E-4 0.0 0.0 10-11 2.852596580638488E-4 0.0 0.0 12-13 3.312692803322116E-4 0.0 0.0 14-15 4.5089429822995464E-4 0.0 0.0 16-17 5.735866242789218E-4 0.0 0.0 18-19 7.300193399913551E-4 0.0 0.0 20-21 8.64980898645219E-4 0.0 0.0 22-23 0.001082759777382136 0.0 0.0 24-25 0.0013649521272947607 0.0 0.0 26-27 0.0016502117853586093 0.0 0.0 28-29 0.001981481065690821 0.0 0.0 30-31 0.0023618272764426196 0.0 0.0 32-33 0.0028403273480335917 0.0 0.0 34-35 0.0034905966760931184 0.0 0.0 36-37 0.004199144859025904 0.0 0.0 38-39 0.0052849719445592636 0.0 0.0 40-41 0.0066959336941223874 0.0 0.0 42-43 0.008484174346286084 0.0 0.0 44-45 0.011030040111802156 0.0 0.0 46-47 0.013950117471767574 0.0 0.0 48-49 0.01794375268466146 0.0 0.0 50-51 0.022590724533766093 0.0 0.0 52-53 0.028642523516131405 0.0 0.0 54-55 0.036494832383265306 0.0 0.0 56-57 0.046475853107348794 0.0 0.0 58-59 0.058876979962748155 0.0 0.0 60-61 0.07412763609063479 0.0 0.0 62-63 0.09285661966200964 0.0 0.0 64-65 0.11608841159938157 0.0 0.0 66-67 0.14570633910760228 0.0 0.0 68-69 0.18235760420658 0.0 0.0 70-71 0.22756972635562445 0.0 0.0 72-73 0.28338246547529966 0.0 0.0 74-75 0.3523447546392729 0.0 0.0 76-77 0.4357172574976974 0.0 0.0 78-79 0.5385364940348831 0.0 0.0 80-81 0.6640047339607082 0.0 0.0 82-83 0.8126311504282759 0.0 0.0 84-85 0.9917650137679193 0.0 0.0 86-87 1.2028203530373527 6.134616302448362E-6 0.0 88-89 1.3839541012915944 1.2269232604896724E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTCACG 1070 5.6800386E-4 98.69556 94-95 CAGTCTA 1250 0.0010536178 84.4834 94-95 GATCAGA 1275 0.0011398236 82.82685 94-95 ACCGAGA 1325 0.0013279079 79.701324 94-95 TTAGGCG 1355 0.0014513299 77.936714 94-95 AGTTCGA 2285 1.6047652E-6 77.02717 94-95 ATACTAG 1440 0.0018476594 73.33628 94-95 ATCCGTG 1470 0.0020051454 71.83962 94-95 TATACTG 530 3.5124336E-4 66.41776 94-95 GGGACTA 1600 0.0028059278 66.00265 94-95 CTTTGTG 186920 0.0 64.16702 1 CCGCAAT 1670 0.0033248588 63.236076 94-95 AGTTTGC 1735 0.003867812 60.867 94-95 TAACTTT 22470 0.0 60.17978 1 TTTGTGT 200350 0.0 59.940117 2 AACTTTG 23115 0.0 58.962917 2 TCACTGA 2995 7.998113E-6 58.766968 94-95 GGAAGCG 1855 0.0050403625 56.929512 94-95 ACTTTGT 54960 0.0 56.333256 1 AACCGAT 2555 2.5832502E-4 55.109844 94-95 >>END_MODULE