FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l02n02_6628.34200000035d5f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l02n02_6628.34200000035d5f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16300938
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGGGGTCCATCCCGGATTACCAATCTCAGATAAACTCCG166440.10210455373795055No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCACG10705.6800386E-498.6955694-95
CAGTCTA12500.001053617884.483494-95
GATCAGA12750.001139823682.8268594-95
ACCGAGA13250.001327907979.70132494-95
TTAGGCG13550.001451329977.93671494-95
AGTTCGA22851.6047652E-677.0271794-95
ATACTAG14400.001847659473.3362894-95
ATCCGTG14700.002005145471.8396294-95
TATACTG5303.5124336E-466.4177694-95
GGGACTA16000.002805927866.0026594-95
CTTTGTG1869200.064.167021
CCGCAAT16700.003324858863.23607694-95
AGTTTGC17350.00386781260.86794-95
TAACTTT224700.060.179781
TTTGTGT2003500.059.9401172
AACTTTG231150.058.9629172
TCACTGA29957.998113E-658.76696894-95
GGAAGCG18550.005040362556.92951294-95
ACTTTGT549600.056.3332561
AACCGAT25552.5832502E-455.10984494-95
Produced by FastQC (version 0.11.2)