FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l02n02_6617.34200000035d28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l02n02_6617.34200000035d28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17036741
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGGGGTCCATCCCGGATTACCAATCTCAGATAAACTCCG252460.14818561836445127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCAAT14250.0201.2108894-95
AGATCGA6808.700905E-5158.1206894-95
CCCGAAT4800.0064894855149.3361894-95
TCGGGCG5200.008243916137.848894-95
GTTCTCG7901.5811482E-4136.1038894-95
TAGCATA9052.716099E-4118.8089194-95
GTTAGCG9102.7763136E-4118.15610594-95
AGAGCGC10154.2869765E-4105.9330694-95
CCCGATA7351.3039637E-497.5256894-95
GCTCGGA11156.2290067E-496.4323494-95
ATCGGAC15201.8041257E-594.3175994-95
TTTTGCG17503.6301262E-581.9215794-95
CTAGTTA13600.001371212579.0603494-95
CTTCGGA15450.002274436469.5935794-95
AATCTGA20758.443997E-569.0904894-95
AGCGACA15950.002580590567.4119594-95
CTTTGTG1518250.065.173731
TTTGTGT1631900.060.701042
AACTTTG176600.057.449062
TAACTTT178150.056.68781
Produced by FastQC (version 0.11.2)