FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l02n02_6471.34200000035cd6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l02n02_6471.34200000035cd6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13607858
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTCG4351.3214467E-5253.6268694-95
TATATCG3200.00178574229.8493594-95
GGCCACG3550.0024363734207.1881394-95
AGATCGA3650.002647579201.5117594-95
CTGAGCG4200.00402932175.123394-95
CCGCTAG4600.0052893655159.895294-95
CACCATG6908.319246E-5159.8951994-95
TCGTCGA4700.00564073156.4931694-95
TCTATAC4750.005822082154.8458794-95
GCCACGA4800.0060072676153.2328894-95
CCGACTG7501.1597565E-4147.1035994-95
GACTAGA7901.4264384E-4139.6552994-95
GTGCTCA5450.008781484134.9574194-95
TCTCGCG5650.009780181130.1801694-95
CATTGAA9152.560061E-4120.57670694-95
CCCGATA6201.3524605E-6118.6319394-95
AACATGA12606.2464496E-6116.7488794-95
CTAAACG10153.868008E-4108.6972294-95
CGATCCT5002.2038406E-573.5517894-95
TTCCGCG10650.069.0627194-95