FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l02n02_6271.34200000035c93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l02n02_6271.34200000035c93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16598639
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACACT10555.1311054E-4101.2511994-95
AGATCGA14451.4496187E-598.5651794-95
CCCTCGG14800.001968383672.1756894-95
GTGTGCA15450.002334232469.1391694-95
CTTTGTG1403850.062.149681
TTTGTGT1517400.057.5762982
ACTTTGT425350.056.375361
TTGTGTT1668150.053.7125973
TGTGTTT1802300.049.94754
TAACTTT175750.048.0789681
TCGAGGA22250.00988855448.00899594-95
AACTTTG180700.046.865452
CCGCAAT30706.0413434E-446.39305594-95
GTGTTTG2026250.044.3865975
TTGAGTC204450.034.1091044
AACTCTA44750.003847499631.8271994-95
GTTTGAG1661800.031.585342
TGTTTGA3079150.031.5321791
TTTGAGT629300.031.4114823
GGTCCGA11450.00579652931.09752894-95
Produced by FastQC (version 0.11.2)