FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l02n02_6205.34200000035c50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l02n02_6205.34200000035c50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16052683
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTT256770.15995456958814921No Hit
CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC180570.1124858691846092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA6558.749339E-10271.3140394-95
TAAATCG8451.0143231E-6168.246894-95
CGATATA5000.0075172656142.1685694-95
GTTATAC5000.0075172656142.1685694-95
ATGCTAG10052.4049004E-6141.4612494-95
TATCGCG5250.008697592135.3986294-95
ATATTAG15851.7166349E-7112.1203194-95
CGCTCAG9603.5508024E-4111.0691894-95
CTATACG10404.8819618E-4102.525494-95
AGCACTA11006.101638E-496.933194-95
AAATTCG19055.7623158E-574.6291694-95
TGCCCCG15050.002118708870.84811494-95
ATCGGAG22151.2157443E-464.1844594-95
TATTAGA16800.003276844563.46810594-95
TATGCTC17050.003474341262.53748794-95
CCCACGA17350.003722892461.45614294-95
ACTTTGT413900.053.773321
CTTTGTG1042150.052.783021
GAGCGCG14851.5214187E-447.868294-95
TTTGTGT1158050.047.5927962
Produced by FastQC (version 0.11.2)