FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l02n02_6019.34200000035bf1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l02n02_6019.34200000035bf1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15647248
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCTCG10505.129783E-4101.2558294-95
TATTATA12058.8669336E-488.2312294-95
ACATCCG12900.001162392482.4175294-95
CCGTTCA13600.001433721278.1754594-95
AACACGA23451.7982293E-675.5640494-95
ATATTAG20508.406452E-569.15031494-95
CTTTGTG1453150.060.505341
GGAAATA17900.004261044359.3958794-95
TATTAGA18200.00455088858.41681794-95
TTTGTGT1582400.055.6460152
ACTTTGT471400.053.5786971
GGAGTAG20050.006675124653.02673794-95
TTGTGTT1789550.050.3432923
GTATAGA22100.00980770148.10796794-95
ACTTCGA37152.7503827E-547.69789594-95
TAACTTT242050.047.3271521
TGTGTTT1956200.046.2784844
AACTTTG252550.045.635832
AGAGCGA36600.001468448738.73173594-95
GTGTTTG2345750.038.6304445
Produced by FastQC (version 0.11.2)