##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l02n02_3522.34200000035b7a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15190797 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.42588469847895 33.0 33.0 33.0 33.0 33.0 2 32.423259622256815 33.0 33.0 33.0 33.0 33.0 3 32.41193605575797 33.0 33.0 33.0 33.0 33.0 4 36.257062746609016 37.0 37.0 37.0 37.0 37.0 5 36.25761663459791 37.0 37.0 37.0 37.0 37.0 6 36.24155500201866 37.0 37.0 37.0 37.0 37.0 7 36.16676761594537 37.0 37.0 37.0 37.0 37.0 8 36.238697284941665 37.0 37.0 37.0 37.0 37.0 9 36.19705700760796 37.0 37.0 37.0 37.0 37.0 10-11 36.18359230921195 37.0 37.0 37.0 37.0 37.0 12-13 36.16691866134476 37.0 37.0 37.0 37.0 37.0 14-15 38.15767579541745 40.0 37.0 40.0 37.0 40.0 16-17 38.13021927025949 40.0 37.0 40.0 37.0 40.0 18-19 38.067730580561374 40.0 37.0 40.0 37.0 40.0 20-21 38.02287256554084 40.0 37.0 40.0 37.0 40.0 22-23 37.988902721825596 40.0 37.0 40.0 37.0 40.0 24-25 37.93787679474619 40.0 37.0 40.0 37.0 40.0 26-27 37.8753409383326 40.0 37.0 40.0 35.0 40.0 28-29 37.80695611296761 40.0 37.0 40.0 33.0 40.0 30-31 37.72796243014767 40.0 37.0 40.0 33.0 40.0 32-33 37.67587461013402 40.0 37.0 40.0 33.0 40.0 34-35 37.58521439000205 40.0 37.0 40.0 33.0 40.0 36-37 37.49282904642857 40.0 37.0 40.0 33.0 40.0 38-39 37.33168634930742 40.0 37.0 40.0 33.0 40.0 40-41 37.23471273429564 40.0 37.0 40.0 33.0 40.0 42-43 37.11030830047956 40.0 37.0 40.0 33.0 40.0 44-45 36.893251223092506 38.5 37.0 40.0 33.0 40.0 46-47 36.69098250736943 37.0 37.0 40.0 33.0 40.0 48-49 36.482983019258306 37.0 37.0 40.0 33.0 40.0 50-51 35.095202904758715 37.0 35.0 38.5 30.0 38.5 52-53 35.471050432706065 37.0 35.0 38.5 33.0 40.0 54-55 36.0740222846767 37.0 37.0 40.0 33.0 40.0 56-57 35.94856053306485 37.0 37.0 40.0 33.0 40.0 58-59 35.718612525728574 37.0 37.0 37.0 33.0 40.0 60-61 35.465161637009565 37.0 35.0 37.0 33.0 40.0 62-63 35.20560474871726 37.0 33.0 37.0 33.0 40.0 64-65 34.963544309097145 37.0 33.0 37.0 33.0 38.5 66-67 34.70432607979687 37.0 33.0 37.0 30.0 37.0 68-69 34.51449914049934 37.0 33.0 37.0 27.0 37.0 70-71 34.33584452481328 37.0 33.0 37.0 27.0 37.0 72-73 34.16868308489673 37.0 33.0 37.0 27.0 37.0 74-75 33.921189191060876 37.0 33.0 37.0 27.0 37.0 76-77 33.853966516700865 37.0 33.0 37.0 27.0 37.0 78-79 33.745252931758614 37.0 33.0 37.0 27.0 37.0 80-81 33.63134462266858 37.0 33.0 37.0 27.0 37.0 82-83 33.525901702195085 37.0 33.0 37.0 27.0 37.0 84-85 33.41425693464274 37.0 33.0 37.0 27.0 37.0 86-87 33.330368643593886 37.0 33.0 37.0 27.0 37.0 88-89 33.228302800702295 37.0 33.0 37.0 27.0 37.0 90-91 33.14718355462192 37.0 33.0 37.0 27.0 37.0 92-93 33.03000477855112 37.0 33.0 37.0 27.0 37.0 94-95 32.89289561304782 35.0 33.0 37.0 27.0 37.0 96-97 32.8057642400198 33.0 33.0 37.0 27.0 37.0 98-99 31.30997386114764 33.0 30.0 37.0 21.0 37.0 100-101 2.714828096248011 2.0 2.0 2.0 2.0 4.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.2050062044703651 2306 2 0.15996150958095967 2306 3 0.20905609636556477 2306 4 0.24427516818548156 2306 5 0.2381920501466368 2306 6 0.24894077595899944 2306 7 0.31625827157733966 2306 8 0.25222766148949205 2306 9 0.27135025849477756 2306 10-11 0.2567283810431391 2306 12-13 0.22505690814299584 2306 14-15 0.3085383986050232 2306 16-17 0.3269285852734356 2306 18-19 0.36101733320206364 2306 20-21 0.33676919309933595 2306 22-23 0.3242396361239841 2306 24-25 0.3184409971829041 2306 26-27 0.27743482027053545 2306 28-29 0.18814197668746857 2306 30-31 0.21697612849062864 2306 32-33 0.26461054524543215 2306 34-35 0.21007054101090006 2306 36-37 0.2789399519213518 2306 38-39 0.28977301765937113 2306 40-41 0.24607401881267776 2306 42-43 0.17934477913588154 2306 44-45 0.22858941427524826 2306 46-47 0.1650378016798726 2306 48-49 0.15408210812426404 2306 50-51 0.13802565541461576 2306 52-53 0.14356618824668743 2306 54-55 0.3610001717995601 2306 56-57 0.29400685969763884 2306 58-59 0.30424026564927686 2306 60-61 0.32183171954447687 2306 62-63 0.26965496762739605 2306 64-65 0.26951701421346996 2306 66-67 0.2375687953070269 2306 68-69 0.23478287186271984 2306 70-71 0.23542911439767522 2306 72-73 0.24933118253115794 2306 74-75 0.2040906406544849 2306 76-77 0.11520196475367328 2306 78-79 0.18648574700076637 2306 80-81 0.20853432032512131 2306 82-83 0.12810035750336368 2306 84-85 0.18353186259210474 2306 86-87 0.12099373283245995 2306 88-89 0.044632187155308145 2306 90-91 -0.059508515916171234 2306 92-93 -0.07211055670267541 2306 94-95 -0.17676991165450318 2306 96-97 -0.11731906974526396 2306 98-99 -0.20319069558819436 2306 100-101 2.30795730619147 2307 1 0.06500959293687458 2307 2 0.04632428859999749 2307 3 0.028596884700242242 2307 4 0.03756775002489121 2307 5 0.013797487598544933 2307 6 0.06047010770310379 2307 7 0.17239968236625458 2307 8 0.10938808022982727 2307 9 0.14375490217193487 2307 10-11 0.08426758279896518 2307 12-13 0.03265152516906511 2307 14-15 0.09670005128985082 2307 16-17 0.09060180457472455 2307 18-19 0.09573695690139061 2307 20-21 -0.028676147582736178 2307 22-23 0.03475963061331555 2307 24-25 -0.019487647415047604 2307 26-27 0.015494136468731767 2307 28-29 -0.05925190568553518 2307 30-31 -0.05479236286304712 2307 32-33 -0.0530040385842554 2307 34-35 -0.07296550457368056 2307 36-37 -0.039966126283836445 2307 38-39 -0.08438631836001775 2307 40-41 -0.08615097602520905 2307 42-43 -0.10991849743181348 2307 44-45 -0.0720990099973875 2307 46-47 -0.09385308302534412 2307 48-49 0.007738512491421545 2307 50-51 -0.14042830820879004 2307 52-53 -0.037137596632902614 2307 54-55 0.05653877638228266 2307 56-57 0.09486294936316852 2307 58-59 0.06253997490517094 2307 60-61 0.15642858578652863 2307 62-63 0.13245056638222508 2307 64-65 0.13220662687601248 2307 66-67 0.22419432111496462 2307 68-69 0.16703526425023085 2307 70-71 0.14373361350246228 2307 72-73 0.12200435556090383 2307 74-75 0.033854736873699665 2307 76-77 0.0815932812898339 2307 78-79 0.04424775090808453 2307 80-81 0.01579947323407538 2307 82-83 0.012538310787974183 2307 84-85 0.06685266703743764 2307 86-87 0.04877995649904676 2307 88-89 0.07260495296241487 2307 90-91 -0.0638868224837097 2307 92-93 -0.15846410071656436 2307 94-95 -0.1205260692437875 2307 96-97 -0.08879682251030374 2307 98-99 0.855283032435306 2307 100-101 4.414402687629998 2201 1 0.09797145408556673 2201 2 0.07497771044381096 2201 3 0.09989361685902054 2201 4 0.11242154463641185 2201 5 0.12196859549020189 2201 6 0.12479711818490102 2201 7 0.19279617113006253 2201 8 0.11169674604807511 2201 9 0.1379167615744734 2201 10-11 0.1599057890614901 2201 12-13 0.15926476878600937 2201 14-15 0.19360177056774575 2201 16-17 0.19925245472145292 2201 18-19 0.1717774183275651 2201 20-21 0.21100360779000482 2201 22-23 0.22502527968271835 2201 24-25 0.2693896222199754 2201 26-27 0.27613097045252033 2201 28-29 0.1945822528648833 2201 30-31 0.1823727792678156 2201 32-33 0.23059605475040712 2201 34-35 0.2329895783156246 2201 36-37 0.2272066180113157 2201 38-39 0.1419555169566138 2201 40-41 0.12289053640812142 2201 42-43 0.12210592176860757 2201 44-45 0.1159012110112485 2201 46-47 0.12858763122425643 2201 48-49 0.11029219717598693 2201 50-51 0.14118285788351415 2201 52-53 0.2207240998180211 2201 54-55 0.27588516179601186 2201 56-57 0.21487442109872745 2201 58-59 0.20558885986581288 2201 60-61 0.15422441708295764 2201 62-63 0.15798072433720023 2201 64-65 0.1314845051194382 2201 66-67 0.10042676522525085 2201 68-69 0.03419066607608556 2201 70-71 0.13987246255778985 2201 72-73 0.03485268978453604 2201 74-75 0.013580878839199784 2201 76-77 0.026483965421512323 2201 78-79 -0.0674403885058581 2201 80-81 -0.10650164418703412 2201 82-83 -0.08575935117141853 2201 84-85 -0.19751204254431087 2201 86-87 -0.14557456663828816 2201 88-89 -0.097278919702255 2201 90-91 -0.19199551963284733 2201 92-93 -0.2237189915534188 2201 94-95 -0.3466239616773876 2201 96-97 -0.31930266509949234 2201 98-99 -0.3414296959168617 2201 100-101 2.14686261762234 1101 1 0.04534703511356497 1101 2 0.05794761772434498 1101 3 0.07938391071263595 1101 4 0.07679840089492984 1101 5 0.10801854627334251 1101 6 0.13238188842932885 1101 7 0.18587817939642548 1101 8 0.12994140590924275 1101 9 0.10701117311699448 1101 10-11 0.1440079422787477 1101 12-13 0.11728378293959452 1101 14-15 0.11250973522212604 1101 16-17 0.20490400008344523 1101 18-19 0.13401501121523296 1101 20-21 0.15107075408921844 1101 22-23 0.12482121919241962 1101 24-25 0.19841026909832493 1101 26-27 0.19314474260114167 1101 28-29 0.138182951490478 1101 30-31 0.12061217901323573 1101 32-33 0.10324148139763167 1101 34-35 0.06360917733904614 1101 36-37 0.11076854942404424 1101 38-39 0.1357709881424185 1101 40-41 0.10470790508904315 1101 42-43 -0.03776075841697235 1101 44-45 0.08105684887992481 1101 46-47 0.09871339470439722 1101 48-49 -0.08705028010437132 1101 50-51 -0.16801668789468494 1101 52-53 0.06807341067048611 1101 54-55 0.18155456040911844 1101 56-57 0.09557373082269294 1101 58-59 0.19781885051628478 1101 60-61 0.20490086262727658 1101 62-63 0.14902408304522652 1101 64-65 0.2060561692724292 1101 66-67 0.24848603968815297 1101 68-69 0.1314317354832042 1101 70-71 0.19417936325902474 1101 72-73 0.18520679079041713 1101 74-75 0.007895889928811073 1101 76-77 0.06974469756051604 1101 78-79 0.061156448731210844 1101 80-81 -0.07428714022442762 1101 82-83 -0.044368590301601785 1101 84-85 -0.07532771062378174 1101 86-87 -0.07323853812058445 1101 88-89 -0.007129554734440546 1101 90-91 -0.08358127367797863 1101 92-93 -0.1526379932941424 1101 94-95 -0.2751522640820312 1101 96-97 -0.15982205222911716 1101 98-99 -0.19665960040101638 1101 100-101 2.0893340033282857 2304 1 0.08556929560607784 2304 2 0.08251872143667072 2304 3 0.0645163974781866 2304 4 0.11058613191999456 2304 5 0.06753240111628145 2304 6 0.08931594665516229 2304 7 0.09823559189957365 2304 8 0.11933496866010529 2304 9 0.0885812058241271 2304 10-11 0.09579955905289239 2304 12-13 0.09012953707014759 2304 14-15 0.09877758517478696 2304 16-17 0.10576127267261626 2304 18-19 0.12205258253302986 2304 20-21 0.08872246130081862 2304 22-23 0.1508256498071745 2304 24-25 0.1338179865378777 2304 26-27 0.15306354637306896 2304 28-29 0.12672207385826795 2304 30-31 0.06404632642944108 2304 32-33 0.10564895360106874 2304 34-35 0.14240426117916627 2304 36-37 0.11461317358807577 2304 38-39 0.07764522694891696 2304 40-41 0.10439362389735862 2304 42-43 0.12804284855079118 2304 44-45 0.09413317607504723 2304 46-47 0.08946314845658065 2304 48-49 0.13167859441816887 2304 50-51 -0.028100664330239056 2304 52-53 0.06652515185747632 2304 54-55 0.1874555857320317 2304 56-57 0.195924442090039 2304 58-59 0.19302935106150443 2304 60-61 0.22056965625171898 2304 62-63 0.23233515805099358 2304 64-65 0.21225997843830413 2304 66-67 0.19957300313934923 2304 68-69 0.14110053456251137 2304 70-71 0.21583664576452577 2304 72-73 0.2239021795342353 2304 74-75 3.491582785741798E-4 2304 76-77 0.0683005440804223 2304 78-79 -0.13005081634699422 2304 80-81 -0.02874093927052712 2304 82-83 -0.0014533779931866775 2304 84-85 0.03502068543009784 2304 86-87 0.003549687379162947 2304 88-89 0.05545084424618807 2304 90-91 -0.09566409745166027 2304 92-93 -0.08709358446314042 2304 94-95 -0.02830935621606301 2304 96-97 -0.09649420916622375 2304 98-99 -0.09523926151128492 2304 100-101 2.1601906335294347 2202 1 0.07597944885608854 2202 2 0.07776219138082041 2202 3 0.06463862222526728 2202 4 0.05436462582565582 2202 5 0.013325527044841579 2202 6 0.032681590782900116 2202 7 0.12439446489766226 2202 8 0.09044894095648459 2202 9 0.08426229998466539 2202 10-11 0.06031286154824045 2202 12-13 0.05286853842368089 2202 14-15 0.04879230261379064 2202 16-17 -0.022408513306096722 2202 18-19 0.04181950710541571 2202 20-21 0.07475032390938452 2202 22-23 0.03138150002879314 2202 24-25 0.06185148015613606 2202 26-27 0.05903631517012542 2202 28-29 0.033583171280334057 2202 30-31 -0.012099068636054255 2202 32-33 -0.005478738305498609 2202 34-35 0.054466555048129806 2202 36-37 0.09027869975717095 2202 38-39 0.08215569144526569 2202 40-41 0.05570830638280455 2202 42-43 0.06592670492680952 2202 44-45 0.025856596628884176 2202 46-47 0.07784114400430298 2202 48-49 -0.07088581257128368 2202 50-51 -0.05886440566708728 2202 52-53 0.057965191118761084 2202 54-55 0.19081609683878753 2202 56-57 0.1726283399209123 2202 58-59 0.14076338453083537 2202 60-61 0.2072111817533795 2202 62-63 0.1343369666739065 2202 64-65 0.14512385211416756 2202 66-67 0.09521263711759786 2202 68-69 0.07843993643560765 2202 70-71 0.18386005000971295 2202 72-73 0.16743201380788975 2202 74-75 -0.024003983983426735 2202 76-77 0.021105230958589516 2202 78-79 0.09038138688544706 2202 80-81 -0.016945767403264256 2202 82-83 0.0015686720258543119 2202 84-85 0.00418900658941368 2202 86-87 0.008454291382463452 2202 88-89 -0.017928111082220255 2202 90-91 -0.01413091053802873 2202 92-93 8.227859444858154E-4 2202 94-95 0.017924220212726993 2202 96-97 -0.2009853744952963 2202 98-99 -0.1355983672321912 2202 100-101 2.180567410427918 1102 1 -0.08447553228339189 1102 2 -0.0907902292895244 1102 3 -0.06917057951380201 1102 4 -0.15637530097533414 1102 5 -0.18094088083886106 1102 6 -0.09258418583784334 1102 7 -0.008636838000278146 1102 8 -0.0422685070537554 1102 9 -0.035101485992456105 1102 10-11 -0.06233649911126804 1102 12-13 -0.10345370646370355 1102 14-15 -0.1398714180290881 1102 16-17 -0.1028962319966169 1102 18-19 -0.02798290098377265 1102 20-21 -0.039958583151758376 1102 22-23 0.004570712004841937 1102 24-25 0.02751930472717845 1102 26-27 0.04822383565485211 1102 28-29 0.030623424596548432 1102 30-31 -0.03822622931284769 1102 32-33 -0.02286119984989199 1102 34-35 -0.02219241719493681 1102 36-37 -0.023452460109261608 1102 38-39 0.03006521381659155 1102 40-41 0.04662702465408586 1102 42-43 0.09090962563622895 1102 44-45 0.04718036112410573 1102 46-47 0.13036920191624546 1102 48-49 0.12244560574666963 1102 50-51 -6.933642820641239E-4 1102 52-53 0.05281107758749215 1102 54-55 0.12758191611968073 1102 56-57 0.11664015530747918 1102 58-59 0.15383353021846347 1102 60-61 0.08161341145711276 1102 62-63 0.1450715149575288 1102 64-65 0.16273259643799776 1102 66-67 0.0987131575324085 1102 68-69 0.07614249068058143 1102 70-71 0.14016031535558682 1102 72-73 0.16582679566636216 1102 74-75 -0.006278262390260636 1102 76-77 0.01849589943324048 1102 78-79 -0.002399709431848862 1102 80-81 0.016098412742415746 1102 82-83 0.023008646127081533 1102 84-85 0.0036200437734947855 1102 86-87 -0.009501107767988515 1102 88-89 -0.027965043486879892 1102 90-91 -0.06912827087283802 1102 92-93 -0.05629114647964428 1102 94-95 0.005338516389862491 1102 96-97 -0.0966920406375138 1102 98-99 0.038758701934494155 1102 100-101 2.392084504962099 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 146.0 3 109.0 4 158.0 5 332.0 6 1787.0 7 13804.0 8 23723.0 9 20357.0 10 15712.0 11 12970.0 12 12298.0 13 12990.0 14 13920.0 15 15609.0 16 17978.0 17 21125.0 18 24278.0 19 28425.0 20 33083.0 21 38637.0 22 45124.0 23 53660.0 24 64228.0 25 78805.0 26 99743.0 27 130676.0 28 151541.0 29 185696.0 30 246424.0 31 337627.0 32 481880.0 33 737919.0 34 1276925.0 35 2738013.0 36 5905453.0 37 2343969.0 38 5673.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.129589172062595 21.242294589323503 30.694168447750698 26.933947790863204 2 28.032729644041478 20.763544614707 31.195035312932472 20.00869042831905 3 22.291990814359583 19.24978270836198 38.35329008165211 20.10493639562633 4 21.316253191349123 19.21701002827529 38.387986534849205 21.078750245526383 5 29.338834403745633 18.64724715234066 32.432034125355685 19.581884318558018 6 30.536637850531474 19.208242412072778 31.181369312229123 19.073750425166622 7 30.17891596000623 25.841133896483086 24.435998135191255 19.54395200831943 8 27.296787582434334 21.390211821636562 30.622461981686282 20.690538614242822 9 23.26216122437657 19.85350115091366 35.07571966861172 21.80861795609805 10-11 28.695912157628307 20.796444066860964 27.977045724332218 22.530598051178515 12-13 27.85733784403802 25.134898570923745 23.75648784473818 23.25127574030005 14-15 27.307747783527457 22.636318708852905 25.62154374108383 24.434389766535812 16-17 26.774432049200463 22.55212326552712 24.789774116454627 25.883670568817784 18-19 26.707604694075716 23.256497482901786 25.4979391660983 24.537958656924197 20-21 25.185350866082246 24.112598665866535 25.817694172092438 24.884356295958785 22-23 25.39306272514087 23.955598124545006 25.36654241753166 25.28479673278246 24-25 25.553050545984924 23.73407837112041 25.24219269423747 25.4706783886572 26-27 25.388071658517504 23.863592927361896 25.3520818592959 25.396253554824703 28-29 25.613496725307787 24.196528916213804 24.69778034781353 25.49219401066487 30-31 26.05528099236632 23.92706804610447 24.50751312716114 25.51013783436807 32-33 25.86921734250172 24.349081511078776 24.504124986156246 25.27757616026326 34-35 25.89946860631021 24.452593308651636 24.21891587513517 25.42902220990298 36-37 25.851833066257555 24.334267373395665 24.49504351836494 25.318856041981835 38-39 25.79734421416201 23.922724997941845 24.605205586067346 25.674725201828796 40-41 25.684303049333746 24.41935678333063 24.236051538783503 25.66028862855212 42-43 25.539592494100926 24.310124804889885 24.516746010321306 25.633536690687887 44-45 25.982259964655757 24.036111064865313 24.692101050717394 25.28952791976154 46-47 25.37574697601508 24.38148108027779 24.66490951170612 25.57786243200101 48-49 25.632186924109877 24.15682038504666 24.5961319171425 25.614860773700958 50-51 25.435916397077495 24.29911649284548 25.17757750232762 25.0873896077494 52-53 25.445303239605565 24.300478163617537 25.09652421834988 25.15769437842702 54-55 25.325617221523895 24.17259187263081 24.686569635350715 25.815221270494582 56-57 25.44650344560605 24.24344541090421 24.869497605005808 25.440553538483933 58-59 25.17150469528383 24.53195817542631 25.089094632409324 25.20744249688053 60-61 25.83610943749716 24.365658193305194 24.34512542787033 25.45310694132732 62-63 25.226958278540756 24.697334032041713 24.89362494520742 25.182082744210117 64-65 25.322249679794396 24.82416086193598 24.83196476642581 25.02162469184381 66-67 25.71616655739023 24.754146146782173 24.524064186984145 25.00562310884345 68-69 25.589552114082565 24.80710651180489 24.841020256506976 24.762321117605566 70-71 25.343729242656742 25.134974856315917 24.49439191182651 25.02690398920083 72-73 25.671053244001968 24.598962157760408 24.572736573166544 25.157248025071077 74-75 25.339410654076318 24.497907278998415 24.597797314103904 25.564884752821364 76-77 25.481792827483297 24.249135966433254 24.600258426130335 25.668812779953115 78-79 25.742142646319877 24.114173709270485 24.596740912515443 25.546942731894195 80-81 25.310721232655816 24.193198954739973 24.83044011916601 25.66563969343821 82-83 25.178126304143877 24.860768324349273 24.68056760700018 25.280537764506672 84-85 25.455030685757123 24.696282791285583 24.440768702637268 25.40791782032003 86-87 25.020288474278345 24.997060622086575 24.770258156270337 25.212392747364742 88-89 25.35531285569036 25.79743375670577 23.94746843767632 24.899784949927543 90-91 25.295420233896575 25.002118819713132 24.083230934715004 25.619230011675292 92-93 24.98676903126535 25.471374817708025 24.15448952719523 25.387366623831387 94-95 25.43576181098554 25.523003629964762 23.640832934408902 25.400401624640796 96-97 25.279325903211287 24.909302657128865 23.98417193916681 25.827199500493037 98-99 25.81476864198098 24.771721685529034 24.300901787126282 25.11260788536371 100-101 33.280595439819386 40.67519145291348 13.794746258368937 12.249466848898193 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 904.0 1 1015.0 2 1206.5 3 1270.0 4 1357.5 5 1383.0 6 1324.0 7 1344.5 8 1432.5 9 1570.5 10 1680.0 11 1996.5 12 2463.0 13 2921.0 14 3333.0 15 3634.0 16 3730.0 17 3688.0 18 3608.5 19 3523.0 20 3483.5 21 3547.0 22 3806.5 23 4131.0 24 4585.0 25 5158.0 26 5922.5 27 6793.5 28 7749.0 29 8787.5 30 10196.5 31 12388.0 32 17739.5 33 25782.5 34 33744.5 35 47926.0 36 68562.0 37 94414.0 38 119255.5 39 141772.0 40 164315.0 41 195810.0 42 240617.5 43 305618.5 44 400774.0 45 521172.0 46 683232.5 47 869241.5 48 1033313.0 49 1088549.5 50 1073873.0 51 1081123.0 52 1077181.0 53 1044336.0 54 950237.5 55 862670.0 56 765289.0 57 600417.0 58 453642.5 59 351275.5 60 272927.0 61 190952.0 62 118454.0 63 69172.5 64 36793.5 65 20648.0 66 12931.0 67 9119.5 68 6862.5 69 5011.0 70 3673.0 71 2835.5 72 2237.5 73 1785.0 74 1418.5 75 1054.5 76 750.0 77 609.0 78 511.5 79 376.5 80 274.5 81 203.5 82 161.5 83 139.5 84 100.5 85 75.0 86 72.5 87 65.0 88 49.0 89 37.0 90 30.5 91 23.5 92 23.5 93 21.5 94 12.5 95 7.5 96 6.5 97 3.5 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.022493882315720498 2 0.04130790504277031 3 0.07825790839019177 4 0.026581883755012987 5 0.032282703797569014 6 0.07540091543583921 7 0.08447878014563687 8 0.07332729151735752 9 0.09320774940248362 10-11 0.10488916414326385 12-13 0.10390830711515663 14-15 0.11764030550865764 16-17 0.09664074900085888 18-19 0.10847686266889092 20-21 0.10929643783667176 22-23 0.11532640453295505 24-25 0.11178149507231254 26-27 0.11225875772021705 28-29 0.1104319937920308 30-31 0.12388421752986364 32-33 0.11183415853690888 34-35 0.13371253661015944 36-37 0.1419872834848626 38-39 0.16806557285967286 40-41 0.13656623809797472 42-43 0.12873913067234063 44-45 0.15966575025655336 46-47 0.15454093685802 48-49 0.15122972152152386 50-51 0.13693159088361198 52-53 0.1478098877892977 54-55 0.16370767116432403 56-57 0.14806991364574223 58-59 0.14175029789417895 60-61 0.14718121768067863 62-63 0.13130976603795047 64-65 0.12444705830773725 66-67 0.12526992494205538 68-69 0.15078537353899205 70-71 0.15122313858844932 72-73 0.14747744966903317 74-75 0.3235281203481292 76-77 0.2827238096855616 78-79 0.30325597794506765 80-81 0.3207698713898948 82-83 0.31572076172171876 84-85 0.2978941789558507 86-87 0.2832899419299725 88-89 0.2764798976643556 90-91 0.26893256489438966 92-93 0.27437335908050114 94-95 0.28845096146041577 96-97 0.26237596355214277 98-99 0.26327124245028094 100-101 88.31027101474662 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5190797E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.218536559139167 #Duplication Level Percentage of deduplicated Percentage of total 1 74.38478103770228 12.807970717412386 2 9.616899049061738 3.311778557236404 3 3.7280364490343345 1.9257399567450317 4 2.180862716005962 1.5020505762404877 5 1.5320387437534515 1.3189732559668224 6 0.9106036590431527 0.9407557436472253 7 0.6569657134078452 0.7918391708089748 8 0.6333446583095556 0.8724214522910868 9 0.6001832308213982 0.9300849211872455 >10 4.10324216812267 14.8044852922216 >50 0.690144501623451 8.446007125640232 >100 0.8303382715568953 31.428270547464415 >500 0.10105554687451335 11.703470341481559 >1k 0.03079928201088756 8.252450795495522 >5k 5.900819414407637E-4 0.6866051187375978 >10k+ 1.1489073041564109E-4 0.2770964274233243 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTGTGTTTGAGGGAGGTAGAATTCCACGTGTAGCAGTGAAATGCGTAG 17135 0.11279855823232975 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.1849279534181123E-4 6.582933074545069E-6 0.0 2 1.1849279534181123E-4 6.582933074545069E-6 0.0 3 1.3824159456544643E-4 6.582933074545069E-6 0.0 4 1.448245276399915E-4 6.582933074545069E-6 0.0 5 1.448245276399915E-4 6.582933074545069E-6 0.0 6 1.5799039378908163E-4 6.582933074545069E-6 0.0 7 1.645733268636267E-4 6.582933074545069E-6 0.0 8 1.7773919301271684E-4 6.582933074545069E-6 0.0 9 1.9748799223635204E-4 6.582933074545069E-6 0.0 10-11 2.6331732298180276E-4 6.582933074545069E-6 6.582933074545069E-6 12-13 3.1598078757816327E-4 6.582933074545069E-6 1.9748799223635204E-5 14-15 3.8839305139815906E-4 6.582933074545069E-6 2.304026576090774E-5 16-17 4.904285140536076E-4 6.582933074545069E-6 3.949759844727041E-5 18-19 6.155042424699638E-4 6.582933074545069E-6 4.608053152181548E-5 20-21 7.636202366472279E-4 6.582933074545069E-6 4.937199805908801E-5 22-23 8.919874316008567E-4 6.582933074545069E-6 5.595493113363308E-5 24-25 0.001066435158076301 6.582933074545069E-6 7.241226381999574E-5 26-27 0.0012836719495362883 6.582933074545069E-6 9.216106304363096E-5 28-29 0.0015338234063690009 6.582933074545069E-6 9.216106304363096E-5 30-31 0.0018695529931707993 6.582933074545069E-6 9.874399611817602E-5 32-33 0.0021921167138235077 6.582933074545069E-6 1.0861839572999362E-4 34-35 0.002712168426712568 6.582933074545069E-6 1.3165866149090138E-4 36-37 0.0033243812026452595 6.582933074545069E-6 1.448245276399915E-4 38-39 0.00413079050427703 6.582933074545069E-6 1.4811599417726405E-4 40-41 0.0051939341958160586 6.582933074545069E-6 1.645733268636267E-4 42-43 0.006451274413054167 6.582933074545069E-6 1.645733268636267E-4 44-45 0.008172711412047702 6.582933074545069E-6 1.6786479340089925E-4 46-47 0.010259501196678488 6.582933074545069E-6 1.7773919301271684E-4 48-49 0.012958503757241967 6.582933074545069E-6 1.8103065954998938E-4 50-51 0.016240095894902683 6.582933074545069E-6 1.8432212608726192E-4 52-53 0.020532168259506066 6.582933074545069E-6 1.8432212608726192E-4 54-55 0.026074997908273015 6.582933074545069E-6 1.90905059161807E-4 56-57 0.03307265576651443 6.582933074545069E-6 1.90905059161807E-4 58-59 0.04185757995449482 6.582933074545069E-6 1.9419652569907953E-4 60-61 0.05356861789411049 6.582933074545069E-6 2.0407092531089712E-4 62-63 0.06822222691804782 6.582933074545069E-6 2.0407092531089712E-4 64-65 0.08675976645596672 6.582933074545069E-6 2.0407092531089712E-4 66-67 0.11023450579979444 6.582933074545069E-6 2.106538583854422E-4 68-69 0.14059828460613358 6.582933074545069E-6 2.3369412414634994E-4 70-71 0.17808808846566773 6.582933074545069E-6 2.3698559068362245E-4 72-73 0.2243364847808841 6.582933074545069E-6 2.40277057220895E-4 74-75 0.2829772526089316 6.582933074545069E-6 2.6331732298180276E-4 76-77 0.3572886926209336 6.582933074545069E-6 2.7319172259362035E-4 78-79 0.4481726666481028 6.582933074545069E-6 2.7977465566816537E-4 80-81 0.5595822260017036 6.582933074545069E-6 2.8306612220543794E-4 82-83 0.695891729709771 6.582933074545069E-6 2.8306612220543794E-4 84-85 0.8604782224395469 6.582933074545069E-6 2.995234548918006E-4 86-87 1.0572750067030716 6.582933074545069E-6 3.2256372065270835E-4 88-89 1.2273747058827789 6.582933074545069E-6 3.291466537272534E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATCGA 440 0.005095434 161.91101 94-95 AGTCTAG 515 0.008157555 138.33173 94-95 CGCCCGA 820 1.8794138E-4 130.3186 94-95 CTTAAGA 900 2.722439E-4 118.73474 94-95 CCACATA 1045 4.9322116E-4 102.259575 94-95 AGAGCGA 1505 1.7703704E-5 94.67221 94-95 CTGGACG 1655 2.8369468E-5 86.091644 94-95 CGATCTA 510 0.0 69.84396 94-95 TGTTAGA 1570 0.0024835095 68.0645 94-95 CTTTGTG 157680 0.0 66.27806 1 GTTTAGA 1705 0.0034439059 62.675224 94-95 TTTGTGT 170355 0.0 61.432087 2 GGCATCG 1740 0.0037325893 61.414516 94-95 CGACCTA 595 3.631603E-5 59.866253 94-95 AGTACGT 1785 0.004129794 59.86625 94-95 ACTTTGT 42775 0.0 58.867096 1 TAACTTT 16280 0.0 58.832523 1 CACTGAG 1860 0.0048606875 57.452293 94-95 AACTTTG 16960 0.0 56.58397 2 TTGTGTT 195215 0.0 54.93203 3 >>END_MODULE