FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l02n02_3522.34200000035b7a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l02n02_3522.34200000035b7a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15190797
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGGGAGGTAGAATTCCACGTGTAGCAGTGAAATGCGTAG171350.11279855823232975No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA4400.005095434161.9110194-95
AGTCTAG5150.008157555138.3317394-95
CGCCCGA8201.8794138E-4130.318694-95
CTTAAGA9002.722439E-4118.7347494-95
CCACATA10454.9322116E-4102.25957594-95
AGAGCGA15051.7703704E-594.6722194-95
CTGGACG16552.8369468E-586.09164494-95
CGATCTA5100.069.8439694-95
TGTTAGA15700.002483509568.064594-95
CTTTGTG1576800.066.278061
GTTTAGA17050.003443905962.67522494-95
TTTGTGT1703550.061.4320872
GGCATCG17400.003732589361.41451694-95
CGACCTA5953.631603E-559.86625394-95
AGTACGT17850.00412979459.8662594-95
ACTTTGT427750.058.8670961
TAACTTT162800.058.8325231
CACTGAG18600.004860687557.45229394-95
AACTTTG169600.056.583972
TTGTGTT1952150.054.932033
Produced by FastQC (version 0.11.2)