FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l02n01_7637.34100000035bcd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l02n01_7637.34100000035bcd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16365827
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA248990.1521401882104705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1619100.060.9848441
TTTGTGT1780800.055.5375062
TTGTGTT1925000.052.6018683
ACTTTGT536650.051.5027431
TGTGTTT2090850.048.6827354
GTGTTTG2249500.045.181735
TAACTTT300050.043.151291
AACTTTG308800.042.2810552
TTTGAGG754850.032.192948
TTGAGGG415700.031.9524259
TGTTTGA3304000.030.7661826
GTTTGAG1825400.030.762827
GTTTGAC658150.027.6543457
TTTGAGT646250.027.5580233
GTTTGAT937400.024.9650542
TTGAGTC243900.024.8875584
TTGAGTG274700.024.3554614
TTTGACG195550.023.4749558
TTGACCC125800.023.2830929
GTTTGAA258300.022.9828012
Produced by FastQC (version 0.11.2)