##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l02n01_6667.34100000035d95.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16958577 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74810687241035 33.0 33.0 33.0 33.0 33.0 2 32.763547554727026 33.0 33.0 33.0 33.0 33.0 3 32.798021437765684 33.0 33.0 33.0 33.0 33.0 4 36.569447424745604 37.0 37.0 37.0 37.0 37.0 5 36.6481916495706 37.0 37.0 37.0 37.0 37.0 6 36.670587396572245 37.0 37.0 37.0 37.0 37.0 7 36.65757887586913 37.0 37.0 37.0 37.0 37.0 8 36.65853479333791 37.0 37.0 37.0 37.0 37.0 9 36.640646499998205 37.0 37.0 37.0 37.0 37.0 10-11 36.6339806694866 37.0 37.0 37.0 37.0 37.0 12-13 36.61506976676168 37.0 37.0 37.0 37.0 37.0 14-15 38.7392797461721 40.0 37.0 40.0 37.0 40.0 16-17 38.710969646804685 40.0 37.0 40.0 37.0 40.0 18-19 38.67992644665882 40.0 37.0 40.0 37.0 40.0 20-21 38.64432894340132 40.0 37.0 40.0 37.0 40.0 22-23 38.601764405114885 40.0 37.0 40.0 37.0 40.0 24-25 38.55594750078382 40.0 37.0 40.0 37.0 40.0 26-27 38.481799947012064 40.0 37.0 40.0 37.0 40.0 28-29 38.411911742359045 40.0 37.0 40.0 37.0 40.0 30-31 38.35705722832759 40.0 37.0 40.0 37.0 40.0 32-33 38.26345341357356 40.0 37.0 40.0 37.0 40.0 34-35 38.19251352869996 40.0 37.0 40.0 37.0 40.0 36-37 38.108042467242385 40.0 37.0 40.0 37.0 40.0 38-39 38.020890726857566 40.0 37.0 40.0 37.0 40.0 40-41 37.90937514981357 40.0 37.0 40.0 35.0 40.0 42-43 37.78447015336252 40.0 37.0 40.0 33.0 40.0 44-45 37.656489751469124 40.0 37.0 40.0 33.0 40.0 46-47 37.46093699371121 40.0 37.0 40.0 33.0 40.0 48-49 37.226948110092025 37.0 37.0 40.0 33.0 40.0 50-51 37.02548279846829 37.0 37.0 40.0 33.0 40.0 52-53 36.81650821882049 37.0 37.0 40.0 33.0 40.0 54-55 36.563485102553116 37.0 37.0 40.0 33.0 40.0 56-57 36.27691424227398 37.0 37.0 40.0 33.0 40.0 58-59 35.993951143424354 37.0 37.0 38.5 33.0 40.0 60-61 35.71795298626766 37.0 35.0 37.0 33.0 40.0 62-63 35.44788206581248 37.0 33.0 37.0 33.0 40.0 64-65 35.20394488287549 37.0 33.0 37.0 33.0 40.0 66-67 34.92493630214375 37.0 33.0 37.0 33.0 38.5 68-69 34.705798782527566 37.0 33.0 37.0 30.0 37.0 70-71 34.46329821776909 37.0 33.0 37.0 27.0 37.0 72-73 34.27330536046745 37.0 33.0 37.0 27.0 37.0 74-75 34.063067113473025 37.0 33.0 37.0 27.0 37.0 76-77 31.044921546188693 33.0 30.0 33.0 24.5 37.0 78-79 33.78083576823692 35.0 33.0 37.0 27.0 37.0 80-81 34.290719675359554 37.0 33.0 37.0 30.0 37.0 82-83 34.289646147787046 37.0 33.0 37.0 33.0 37.0 84-85 34.22179859194554 37.0 33.0 37.0 27.0 37.0 86-87 34.10645347778885 37.0 33.0 37.0 27.0 37.0 88-89 33.974277558783385 37.0 33.0 37.0 27.0 37.0 90-91 33.89397500745493 37.0 33.0 37.0 27.0 37.0 92-93 33.811932097840526 37.0 33.0 37.0 27.0 37.0 94-95 33.708590555681646 37.0 33.0 37.0 27.0 37.0 96-97 33.56379815948001 37.0 33.0 37.0 27.0 37.0 98-99 33.41535318676797 37.0 33.0 37.0 27.0 37.0 100-101 31.400797956102096 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.09712748007451921 2306 2 0.06261569181128124 2306 3 0.04209987402094839 2306 4 -0.0996430975113114 2306 5 -0.06789890541107724 2306 6 0.01909291714847683 2306 7 0.03957152809856268 2306 8 0.0489927938677468 2306 9 0.05829382415545581 2306 10-11 0.09414430498211601 2306 12-13 0.08173329003382435 2306 14-15 0.1434681504207802 2306 16-17 0.1446751004468112 2306 18-19 0.14922280493353668 2306 20-21 0.1498858129249072 2306 22-23 0.16699845005560832 2306 24-25 0.09399533739161114 2306 26-27 0.0946097517054838 2306 28-29 0.11509922077681978 2306 30-31 0.03366895852992968 2306 32-33 0.14214287079865784 2306 34-35 0.12102805296868269 2306 36-37 0.15771796619618073 2306 38-39 0.04031891711462521 2306 40-41 0.04929641561141551 2306 42-43 0.11129766570176258 2306 44-45 0.017829761947027123 2306 46-47 0.013204837932605074 2306 48-49 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40-41 -0.2813357948078732 1213 42-43 -0.18973513721866908 1213 44-45 -0.19535608317845998 1213 46-47 -0.16039903355834895 1213 48-49 -0.1072030698645321 1213 50-51 -0.09618865686484668 1213 52-53 -0.08115984812259569 1213 54-55 -0.05284841168881371 1213 56-57 -0.13905768151883535 1213 58-59 -0.11632836190854334 1213 60-61 -0.07083507681231538 1213 62-63 -0.04272995992190687 1213 64-65 0.0028549686478100966 1213 66-67 -0.020390541146653618 1213 68-69 -0.01126121029059135 1213 70-71 0.004173966925733907 1213 72-73 0.046294271543843024 1213 74-75 0.12870577827360563 1213 76-77 0.42439089508706473 1213 78-79 -0.09543109369240454 1213 80-81 -0.11508366758669553 1213 82-83 -0.04006541041970024 1213 84-85 0.04957270781208223 1213 86-87 0.04081087428892971 1213 88-89 0.0871226758567687 1213 90-91 0.03477091057757775 1213 92-93 0.097398159697903 1213 94-95 0.155885606971772 1213 96-97 -0.0028962080306698113 1213 98-99 0.18713489939306527 1213 100-101 0.9751364958178108 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 5.0 7 41.0 8 379.0 9 1055.0 10 784.0 11 261.0 12 305.0 13 653.0 14 1343.0 15 2998.0 16 5358.0 17 7538.0 18 10440.0 19 13970.0 20 18009.0 21 23183.0 22 29912.0 23 38094.0 24 48671.0 25 62580.0 26 79655.0 27 102017.0 28 130779.0 29 167906.0 30 217849.0 31 289331.0 32 398717.0 33 580516.0 34 937217.0 35 1836438.0 36 4360588.0 37 6471106.0 38 1120767.0 39 112.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.72566749554759 22.125210457739236 30.45388138508529 27.695240661627885 2 26.86501939402109 22.53144824592299 31.208408582866358 19.39512377718956 3 20.888835241826907 20.569445217570152 38.06980020020549 20.47191934039745 4 20.877089515464014 20.858363435452613 36.885457506469805 21.37908954261357 5 29.212120804711383 20.194966830058913 30.918944437378208 19.673967927851493 6 28.780917172472662 20.735460292452604 31.47401459450283 19.0096079405719 7 29.27625354415055 26.388157449767156 24.680590830232983 19.654998175849308 8 25.78412092005125 22.02147621230248 31.592296924441243 20.602105943205025 9 22.184119575598828 21.155354013488278 35.17475552341449 21.485770887498404 10-11 27.757479297938737 21.99482598097706 28.336525523338423 21.911169197745778 12-13 26.27358586527181 26.234670311639906 24.796534366364035 22.695209456724246 14-15 26.217922057965122 23.846213629834626 26.061768036315787 23.87409627588447 16-17 25.99139361751873 23.545280361671857 25.550259906830625 24.913066113978786 18-19 25.796854889416725 24.26292606979937 26.066128661620606 23.8740903791633 20-21 24.574106935260527 24.78848268845769 26.48956947416644 24.14784090211534 22-23 24.898658984915464 24.971946891756293 25.94273082410581 24.18666329922243 24-25 25.117080873000138 24.7219799161215 25.77479820388232 24.38614100699605 26-27 24.832889840855724 24.764154698384427 25.9086907896855 24.494264671074347 28-29 24.888900826529614 25.2043945875628 25.625761528276946 24.280943057630637 30-31 25.073643853588713 25.055525162807275 25.253872490531844 24.616958493072175 32-33 24.92752648909772 25.330114188532555 25.43764801343561 24.304711308934117 34-35 24.72810214205704 25.38587752490798 25.519551356924875 24.366468976110102 36-37 25.03203947657501 25.14052161429772 25.662015002773543 24.165423906353723 38-39 25.15955830538328 24.922608014370066 25.55693978806793 24.360893892178726 40-41 24.47099374526984 25.171916399277194 25.55086889894157 24.8062209565114 42-43 24.911662532963554 25.08626022188163 25.406102902036793 24.595974343118026 44-45 24.894691434034513 24.982735793154312 25.499880679284253 24.622692093526922 46-47 24.43480455638037 25.270131129211453 25.47127140373597 24.823792910672204 48-49 24.486503567792177 24.98184015497266 25.51720137968529 25.01445489754987 50-51 24.374696211849486 25.319923348374374 25.935779553566913 24.36960088620923 52-53 24.55768911964095 25.06484151682942 25.856990507917892 24.52047885561173 54-55 24.460326504322836 25.395145374522095 25.242863984843822 24.90166413631125 56-57 24.70245637892322 25.35944201404261 25.66420183013972 24.27389977689445 58-59 24.60805953554562 25.548565069951206 25.337066336988396 24.50630905751478 60-61 25.064253982428635 25.54417296323598 25.027404287747306 24.364168766588076 62-63 24.41519898768721 25.815241238158194 25.49070466047937 24.278855113675235 64-65 24.896300692925184 25.530089623923274 25.215870032836367 24.35773965031517 66-67 24.727472491109662 25.623317637898207 25.564734401970124 24.084475469022003 68-69 24.580005104029627 25.525928727089635 25.5858758588276 24.30819031005314 70-71 24.37369888657842 25.722648097764218 25.42965794180875 24.47399507384862 72-73 24.132437939532455 25.45274119600462 25.922768376654105 24.49205248780882 74-75 24.40430374534218 25.2262780852883 25.7208943334232 24.64852383594632 76-77 24.358817153302404 25.541760602160966 25.332538950420496 24.766883294116134 78-79 24.58966045310743 25.225850117547395 25.578120744428688 24.606368684916482 80-81 24.525150952422738 25.401634527647953 25.37417871408068 24.69903580584862 82-83 24.50827861702235 25.962324612281435 25.274273550552728 24.25512322014349 84-85 24.337527633671833 25.728929419224457 25.527219760227343 24.40632318687637 86-87 24.54778746900247 25.922027760031384 25.272823386646444 24.257361384319704 88-89 24.351609587475465 26.63672682065734 24.862014502435507 24.149649089431684 90-91 24.491768255898013 26.13453666583625 24.903822182542125 24.46987289572361 92-93 24.378348524967258 26.463660064916194 24.614608216514632 24.543383193601915 94-95 24.44504890834184 26.569878777527833 24.54930467596408 24.435767638166247 96-97 24.353081287938576 26.24513970237727 24.344321935744873 25.057457073939283 98-99 24.57858451391165 26.269206594634003 24.372063544713978 24.78014534674037 100-101 24.971705463495155 26.834185533779454 23.679295208764234 24.514813793961157 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6621.0 1 4118.5 2 2339.0 3 2719.0 4 2689.5 5 2453.0 6 1853.0 7 1800.0 8 1889.5 9 1940.0 10 1902.5 11 1899.0 12 1924.5 13 1960.5 14 2060.0 15 2274.5 16 2612.5 17 2966.0 18 3317.5 19 3696.0 20 4140.0 21 4993.0 22 6048.0 23 7126.0 24 8513.0 25 10114.5 26 11967.5 27 14141.5 28 17078.5 29 21403.5 30 27472.5 31 35135.0 32 46906.5 33 63853.0 34 84694.0 35 112348.5 36 142868.5 37 180661.5 38 225984.5 39 271488.5 40 323872.5 41 375776.0 42 413895.5 43 455689.0 44 529313.0 45 637043.5 46 773656.0 47 933738.0 48 1063355.0 49 1153620.5 50 1196443.0 51 1166344.0 52 1068926.0 53 946912.5 54 820467.0 55 731607.5 56 691214.0 57 604622.0 58 495001.0 59 398619.5 60 290775.5 61 189789.5 62 122299.0 63 78230.5 64 48395.5 65 29454.0 66 18498.0 67 12879.5 68 10074.0 69 7637.5 70 5769.0 71 4184.5 72 3050.5 73 2443.5 74 1957.5 75 1500.5 76 1156.5 77 863.5 78 647.5 79 529.0 80 396.5 81 278.5 82 210.5 83 151.5 84 110.0 85 82.5 86 75.0 87 68.5 88 55.5 89 51.5 90 37.0 91 31.0 92 33.5 93 29.0 94 20.5 95 13.0 96 7.5 97 4.5 98 4.5 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12787039855997354 2 0.0 3 5.896721169470764E-6 4 0.5251384004683884 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 3.6559671250718735E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.122819621009475E-4 22-23 6.928647374128148E-4 24-25 0.0 26-27 1.7690163508412293E-5 28-29 4.7173769355766113E-4 30-31 0.005601885110997226 32-33 0.003874145808342292 34-35 0.0029984827146758834 36-37 0.021888628981075478 38-39 0.019653771657846056 40-41 0.009260800596653834 42-43 0.006023500674614385 44-45 4.7173769355766113E-4 46-47 0.00741217851002475 48-49 0.008971861259349767 50-51 0.01096200465404615 52-53 0.013173275092597685 54-55 0.02616375182894178 56-57 0.03178627546403215 58-59 0.02279377568058924 60-61 0.026945067383896657 62-63 0.01505432914565886 64-65 0.026594212474313143 66-67 0.029020713235550364 68-69 0.03128505416462714 70-71 0.034136118850066254 72-73 0.03691052616030224 74-75 0.03561914422418815 76-77 0.046545768551217476 78-79 0.016882312708194798 80-81 0.013214552140783983 82-83 0.016428265178145548 84-85 0.019258691339491513 86-87 0.030721917292942682 88-89 0.03686630075153122 90-91 0.03074255581703583 92-93 0.026909687056879832 94-95 0.029946498459157277 96-97 0.03106687548135672 98-99 0.020037058533861656 100-101 0.2417655679483013 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6958577E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.648979990399816 #Duplication Level Percentage of deduplicated Percentage of total 1 71.9161850957696 16.288282372199753 2 13.062884403672676 5.917220149513765 3 4.58375415453154 3.1145206838069073 4 2.360139393970352 2.1381899963435544 5 1.533214984401788 1.736287775134863 6 1.0494891387546206 1.4261915102277205 7 0.732895810954374 1.1619539790147424 8 0.6414483242105441 1.1622520207936087 9 0.42915358405370485 0.8747901844236655 >10 2.7766434349450826 12.343908239488988 >50 0.34027758676663167 5.5485783369496655 >100 0.4497055217189226 21.877250992880974 >500 0.08942455988373503 14.073638665367309 >1k 0.03446021129519923 11.830384987777188 >5k 3.2379507117240145E-4 0.5065501060772911 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.1793442338941528E-4 0.0 0.0 2 1.1793442338941528E-4 0.0 0.0 3 1.1793442338941528E-4 0.0 0.0 4 1.1793442338941528E-4 0.0 0.0 5 1.2383114455888605E-4 0.0 0.0 6 1.2972786572835681E-4 0.0 0.0 7 1.3562458689782758E-4 0.0 0.0 8 1.3562458689782758E-4 0.0 0.0 9 1.6510819274518138E-4 0.0 0.0 10-11 2.0343688034674134E-4 0.0 0.0 12-13 3.0368114022774433E-4 0.0 0.0 14-15 3.773901548461289E-4 0.0 0.0 16-17 4.953245782355442E-4 0.0 0.0 18-19 6.751745739044025E-4 0.0 0.0 20-21 8.196442425564362E-4 0.0 0.0 22-23 9.78855714132147E-4 0.0 0.0 24-25 0.0011911376762330944 0.0 0.0 26-27 0.001527250782892928 0.0 0.0 28-29 0.0018633638895527616 0.0 0.0 30-31 0.0021699933903652413 0.0 0.0 32-33 0.002591608953982401 0.0 0.0 34-35 0.003296267133734157 0.0 0.0 36-37 0.004316399896052599 0.0 0.0 38-39 0.005660852322691933 0.0 0.0 40-41 0.0076126670297867564 0.0 0.0 42-43 0.010165947296167595 0.0 0.0 44-45 0.013692186555511115 0.0 0.0 46-47 0.018397770048748784 0.0 0.0 48-49 0.024704313339497765 0.0 0.0 50-51 0.03279756314459639 0.0 0.0 52-53 0.04347652518250794 0.0 0.0 54-55 0.056576091260487245 0.0 0.0 56-57 0.07342007528108048 0.0 0.0 58-59 0.09534113622858804 0.0 0.0 60-61 0.12323262736018475 0.0 0.0 62-63 0.15741886834019153 0.0 0.0 64-65 0.20090423860445367 0.0 0.0 66-67 0.25600909793315796 0.0 0.0 68-69 0.3255756659299893 5.896721169470764E-6 0.0 70-71 0.41049729585212247 5.896721169470764E-6 0.0 72-73 0.5150314203839155 5.896721169470764E-6 0.0 74-75 0.644036937769012 5.896721169470764E-6 0.0 76-77 0.8013083880799668 1.1793442338941528E-5 0.0 78-79 0.9930137416600462 1.1793442338941528E-5 0.0 80-81 1.2254801803240922 1.1793442338941528E-5 0.0 82-83 1.500464926980607 1.1793442338941528E-5 0.0 84-85 1.826957533052449 1.1793442338941528E-5 0.0 86-87 2.2067122730875357 1.1793442338941528E-5 0.0 88-89 2.6476366501741273 1.1793442338941528E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 164730 0.0 74.14498 1 TTTGTGT 178780 0.0 68.42768 2 TTGTGTT 197420 0.0 63.434277 3 TGTGTTT 209705 0.0 59.98442 4 GTGTTTG 230195 0.0 54.6357 5 TAACTTT 20820 0.0 48.221306 1 ACTTTGT 36090 0.0 47.401245 1 AACTTTG 21485 0.0 47.136932 2 TTTGAGG 61305 0.0 40.722645 8 TTGAGGG 32510 0.0 39.0895 9 GTTTGAG 173325 0.0 38.680714 7 TGTTTGA 339400 0.0 37.074684 6 TTGAGGC 11170 0.0 36.931362 9 TTTGAGC 42305 0.0 35.369038 8 TTTGAGT 66970 0.0 33.608166 3 TTTGAGA 23495 0.0 30.994072 8 TTTGATG 27755 0.0 30.46151 8 GTTTGAT 98090 0.0 30.222923 7 GTTTGAC 74785 0.0 30.005428 7 TTGAGGT 19800 0.0 29.633436 9 >>END_MODULE