FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l02n01_6667.34100000035d95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l02n01_6667.34100000035d95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16958577
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1647300.074.144981
TTTGTGT1787800.068.427682
TTGTGTT1974200.063.4342773
TGTGTTT2097050.059.984424
GTGTTTG2301950.054.63575
TAACTTT208200.048.2213061
ACTTTGT360900.047.4012451
AACTTTG214850.047.1369322
TTTGAGG613050.040.7226458
TTGAGGG325100.039.08959
GTTTGAG1733250.038.6807147
TGTTTGA3394000.037.0746846
TTGAGGC111700.036.9313629
TTTGAGC423050.035.3690388
TTTGAGT669700.033.6081663
TTTGAGA234950.030.9940728
TTTGATG277550.030.461518
GTTTGAT980900.030.2229237
GTTTGAC747850.030.0054287
TTGAGGT198000.029.6334369