FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l02n01_6628.34100000035d52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l02n01_6628.34100000035d52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16300938
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1763550.065.6604841
TTTGTGT1886400.061.5031782
TAACTTT229100.060.027511
AACTTTG237250.058.5751842
ACTTTGT523450.055.9133531
TTGTGTT2144100.055.629083
TGTGTTT2302250.052.0958444
GTGTTTG2656700.045.1473125
TTTGAGG817050.034.100038
TTGAGGG452050.032.9853179
GTTTGAG2063300.032.491757
TGTTTGA3720500.032.219336
TTGAGTC256450.031.8215944
GTTTGAA214950.031.2825452
TTTGAGT800400.030.6884963
TTGAGGC149400.029.6780629
TGAGTCT35400.028.5636865
TTGATTC103750.028.5188584
GTTTGAC756450.028.1400347
TCTGTGT21750.027.6464772