##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l02n01_6617.34100000035d2b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17036741 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.75123663616181 33.0 33.0 33.0 33.0 33.0 2 32.76595318318216 33.0 33.0 33.0 33.0 33.0 3 32.80009163724447 33.0 33.0 33.0 33.0 33.0 4 36.5688705956145 37.0 37.0 37.0 37.0 37.0 5 36.64760836594276 37.0 37.0 37.0 37.0 37.0 6 36.674727461079556 37.0 37.0 37.0 37.0 37.0 7 36.661283868786875 37.0 37.0 37.0 37.0 37.0 8 36.66322320683281 37.0 37.0 37.0 37.0 37.0 9 36.64324514882277 37.0 37.0 37.0 37.0 37.0 10-11 36.63814173731936 37.0 37.0 37.0 37.0 37.0 12-13 36.62221870955249 37.0 37.0 37.0 37.0 37.0 14-15 38.75585985019083 40.0 37.0 40.0 37.0 40.0 16-17 38.72444316081344 40.0 37.0 40.0 37.0 40.0 18-19 38.701080124420514 40.0 37.0 40.0 37.0 40.0 20-21 38.66193375834028 40.0 37.0 40.0 37.0 40.0 22-23 38.62119926575159 40.0 37.0 40.0 37.0 40.0 24-25 38.58259757544005 40.0 37.0 40.0 37.0 40.0 26-27 38.51253256124514 40.0 37.0 40.0 37.0 40.0 28-29 38.450022219625225 40.0 37.0 40.0 37.0 40.0 30-31 38.39732772247932 40.0 37.0 40.0 37.0 40.0 32-33 38.303615902830245 40.0 37.0 40.0 37.0 40.0 34-35 38.23312108225393 40.0 37.0 40.0 37.0 40.0 36-37 38.15045104577219 40.0 37.0 40.0 37.0 40.0 38-39 38.05787676762827 40.0 37.0 40.0 37.0 40.0 40-41 37.95439230425585 40.0 37.0 40.0 35.0 40.0 42-43 37.83338714839886 40.0 37.0 40.0 33.0 40.0 44-45 37.7036403265155 40.0 37.0 40.0 33.0 40.0 46-47 37.513985656059454 40.0 37.0 40.0 33.0 40.0 48-49 37.282177060741844 38.5 37.0 40.0 33.0 40.0 50-51 37.08397442327732 37.0 37.0 40.0 33.0 40.0 52-53 36.8700457734258 37.0 37.0 40.0 33.0 40.0 54-55 36.62531446008365 37.0 37.0 40.0 33.0 40.0 56-57 36.34499062936978 37.0 37.0 40.0 33.0 40.0 58-59 36.05020578759752 37.0 37.0 40.0 33.0 40.0 60-61 35.76355190232686 37.0 35.0 37.0 33.0 40.0 62-63 35.50035558443953 37.0 33.0 37.0 33.0 40.0 64-65 35.23825783346709 37.0 33.0 37.0 33.0 40.0 66-67 34.94412813460039 37.0 33.0 37.0 33.0 38.5 68-69 34.714189468513965 37.0 33.0 37.0 30.0 37.0 70-71 34.46252396511751 37.0 33.0 37.0 27.0 37.0 72-73 34.27751146771557 37.0 33.0 37.0 27.0 37.0 74-75 34.06632445137248 37.0 33.0 37.0 27.0 37.0 76-77 31.069203112261903 33.0 30.0 33.0 24.5 37.0 78-79 33.79144224238662 35.0 33.0 37.0 27.0 37.0 80-81 34.287271432957745 37.0 33.0 37.0 30.0 37.0 82-83 34.289517167632 37.0 33.0 37.0 30.0 37.0 84-85 34.21960743548311 37.0 33.0 37.0 27.0 37.0 86-87 34.09396738495937 37.0 33.0 37.0 27.0 37.0 88-89 33.94695364565324 37.0 33.0 37.0 27.0 37.0 90-91 33.87363008570712 37.0 33.0 37.0 27.0 37.0 92-93 33.78473705739848 37.0 33.0 37.0 27.0 37.0 94-95 33.68183642047502 37.0 33.0 37.0 27.0 37.0 96-97 33.54111167740356 37.0 33.0 37.0 27.0 37.0 98-99 33.389390670433976 37.0 33.0 37.0 27.0 37.0 100-101 31.369042705996414 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.09756518433962924 2306 2 0.07614013687038579 2306 3 0.06760486886801687 2306 4 -0.10149544029032853 2306 5 -0.04452713505283867 2306 6 0.024101484219279712 2306 7 0.06695886603421997 2306 8 0.08955876834596666 2306 9 0.09278421719219665 2306 10-11 0.1054281999359361 2306 12-13 0.08066963241429903 2306 14-15 0.1739281793812566 2306 16-17 0.1747437385784778 2306 18-19 0.1979504429539105 2306 20-21 0.19066680771190647 2306 22-23 0.20097794223480037 2306 24-25 0.1417188494371402 2306 26-27 0.12180578025308364 2306 28-29 0.12052630770232753 2306 30-31 0.11621496708268353 2306 32-33 0.1950504879536652 2306 34-35 0.2021337327359518 2306 36-37 0.1764955921325111 2306 38-39 0.06764345684451456 2306 40-41 0.15686515261847944 2306 42-43 0.11049950341534043 2306 44-45 0.01881031445154946 2306 46-47 -0.0016148732771981145 2306 48-49 0.07603236028855775 2306 50-51 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46-47 -0.1942432539946921 1213 48-49 -0.1342554636529698 1213 50-51 -0.13950106223119718 1213 52-53 -0.12429133924432278 1213 54-55 -0.0714495806276716 1213 56-57 -0.16379689247113305 1213 58-59 -0.14863300144651248 1213 60-61 -0.15266277255067706 1213 62-63 -0.1045349923750436 1213 64-65 -0.009217488860187473 1213 66-67 0.006002876177220173 1213 68-69 0.0014802745594764133 1213 70-71 -0.008822583474909607 1213 72-73 0.05783314249087823 1213 74-75 0.07636060221204133 1213 76-77 0.3841998716341237 1213 78-79 -0.10252672710945632 1213 80-81 -0.13420237922576916 1213 82-83 -0.06858043562155558 1213 84-85 -0.010206810314315362 1213 86-87 0.006524208762094474 1213 88-89 0.05965552670598129 1213 90-91 -0.032183157066576484 1213 92-93 0.03094185362825641 1213 94-95 0.059824046638716766 1213 96-97 0.0035609170127557377 1213 98-99 0.1419148965732262 1213 100-101 0.9211037933150408 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 3.0 7 45.0 8 477.0 9 1332.0 10 879.0 11 342.0 12 351.0 13 577.0 14 1136.0 15 2348.0 16 3995.0 17 5572.0 18 8037.0 19 10828.0 20 14867.0 21 19839.0 22 26226.0 23 34884.0 24 45589.0 25 60069.0 26 77442.0 27 101108.0 28 131800.0 29 171698.0 30 225342.0 31 301968.0 32 412198.0 33 593798.0 34 949251.0 35 1847025.0 36 4341388.0 37 6473101.0 38 1173186.0 39 40.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 20.56593584975832 22.93970966207799 30.243864624319393 26.2504898638443 2 26.811213482672535 23.087044640756115 29.598090385948815 20.50365149062253 3 21.572127154146106 21.10787751106594 36.241835952291105 21.07815938249685 4 21.629638817670642 21.32628135012484 35.071670916157494 21.972408916047026 5 28.428993549881397 20.53808295847193 30.768055932763193 20.264867558883473 6 29.14311487155906 21.234430927840016 30.02529650477166 19.597157695829267 7 28.212461526532568 26.110621743912173 25.415594449666166 20.261322279889093 8 26.770718648596 22.369771307787094 29.832495545949776 21.02701449766713 9 23.08716790376751 21.594981105834737 33.58212700421988 21.735723986177874 10-11 27.21549268137609 22.42019174911446 28.135319425235146 22.22899614427431 12-13 26.489006611259203 25.885347826286015 24.7317848169369 22.89386074551788 14-15 26.316068900736354 23.94180905843436 25.937134925042294 23.804987115786993 16-17 25.634483144399507 23.719621610729423 25.559424774961357 25.08647046990971 18-19 25.6889272425988 24.260003717847212 26.01926917830118 24.031799861252807 20-21 24.59000574172161 24.73816528574603 26.647185000946777 24.02464397158558 22-23 24.685834232327796 24.948306962405177 25.879456655357895 24.486402149909132 24-25 25.331182765530098 24.739361829824144 25.60484132499285 24.32461407965291 26-27 25.020562179752766 24.813753241927543 26.045884542637985 24.11980003568171 28-29 25.137429977537874 25.25858624064929 25.352454316792784 24.251529465020056 30-31 25.210080112056033 25.258669071646512 25.176415638090294 24.354835178207164 32-33 25.02575715045616 25.657987187872116 25.169405506418567 24.146850155253162 34-35 24.86124679454312 25.65212524425219 25.241431722117564 24.245196239087125 36-37 24.974689466934592 25.455998362231135 25.42938520525111 24.139926965583165 38-39 24.907281159980826 25.257485308013827 25.51903396809688 24.316199563908462 40-41 24.64653901436585 25.541435506205712 25.11304784467907 24.698977634749365 42-43 24.524985603045085 25.355836872294308 25.39761086124887 24.721566663411743 44-45 24.86442389182356 25.320238308608644 25.207857050053008 24.60748074951479 46-47 24.444383134412444 25.30404011750198 25.402508268416035 24.849068479669537 48-49 24.65546649034165 24.941104329339566 25.266421754286768 25.137007426032014 50-51 24.442530460122217 25.244923577128294 25.77272673505958 24.53981922768991 52-53 24.62260023064473 25.26836027060147 25.796620003019182 24.312419495734623 54-55 24.50057972382741 25.124528482675924 25.290252349543056 25.08463944395361 56-57 24.46160939490149 25.636984930996302 25.494601730564035 24.406803943538172 58-59 24.345114350607012 25.817993720382564 25.630478222642644 24.20641370636778 60-61 25.048368434116252 25.441789006035837 24.96207512581811 24.5477674340298 62-63 24.511072976405128 25.757035440845122 25.498873272171362 24.233018310578387 64-65 24.745839317013925 25.380024570678778 25.494878358876637 24.379257753430664 66-67 25.023652972465555 25.613518238887213 25.486369934250785 23.876458854396446 68-69 24.867085922464874 25.197288542543028 25.98153701297609 23.954088522016008 70-71 24.642211578154964 25.848554532515134 25.481492455082993 24.027741434246913 72-73 24.505799382166103 25.521500480422972 25.808121880138906 24.164578257272016 74-75 24.590055786816063 25.548013023138104 25.689885312828505 24.172045877217332 76-77 24.42596149244169 25.374718011252245 25.607919732708577 24.591400763597488 78-79 24.66720140736645 25.35343403389531 25.243247235305848 24.736117323432392 80-81 24.54293830941065 25.198691335247336 25.61262574656012 24.645744608781893 82-83 24.358549565910636 25.722541503181738 25.6362930820154 24.282615848892224 84-85 24.51507126096355 25.504017728954427 25.368909480614505 24.612001529467523 86-87 24.342741668186903 25.70248971350836 25.488577894475217 24.46619072382952 88-89 24.418359635256586 26.863246058763135 24.765113847482347 23.953280458497932 90-91 24.444657059512277 25.877486572443487 24.890888396925416 24.786967971118823 92-93 24.202957659356287 26.299617916622964 24.908925194437497 24.588499229583253 94-95 24.318803295727886 26.429343844480584 24.642698350418645 24.609154509372885 96-97 24.151147106122398 25.938963724856585 24.955867887317012 24.954021281704 98-99 24.53514438762876 25.62636622520452 24.854872054334212 24.98361733283251 100-101 24.716416282257235 26.509284842533837 24.349447913174284 24.42485096203464 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1505.0 1 1487.5 2 1570.5 3 1611.5 4 1663.0 5 1654.0 6 1535.5 7 1551.5 8 1585.5 9 1613.0 10 1661.5 11 1735.0 12 1861.0 13 2036.0 14 2214.5 15 2400.0 16 2571.5 17 2801.0 18 3176.0 19 3577.0 20 4012.5 21 4565.5 22 5109.5 23 5644.0 24 6525.0 25 7814.0 26 9168.5 27 10806.5 28 12780.5 29 15067.5 30 17484.0 31 20472.0 32 25284.0 33 32304.5 34 41482.5 35 54687.0 36 74549.5 37 99014.5 38 125926.5 39 168865.5 40 243009.0 41 351980.5 42 458451.0 43 524723.0 44 611025.0 45 783635.5 46 973311.5 47 1132031.0 48 1281792.0 49 1330231.5 50 1266338.0 51 1168681.0 52 1051757.5 53 959370.0 54 878665.0 55 790813.0 56 691602.0 57 510700.5 58 329813.5 59 232223.0 60 180114.0 61 152730.5 62 126829.5 63 91669.0 64 55537.5 65 33573.5 66 19041.0 67 10071.0 68 5907.0 69 3168.0 70 1991.0 71 1345.0 72 985.5 73 744.5 74 551.5 75 424.0 76 317.5 77 218.5 78 156.5 79 130.0 80 102.5 81 75.0 82 62.0 83 58.0 84 45.0 85 29.5 86 19.0 87 10.5 88 10.0 89 12.0 90 11.0 91 5.5 92 5.0 93 5.0 94 4.0 95 2.0 96 2.0 97 2.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12801157216629636 2 0.0 3 1.1739334418478275E-5 4 0.5306003067135904 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 2.758743588342395E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.2891702116032637E-4 22-23 6.691420618532617E-4 24-25 0.0 26-27 8.804500813858707E-6 28-29 3.81528368600544E-4 30-31 0.005740534530635877 32-33 0.004079418710421201 34-35 0.0032752743027554387 36-37 0.022378106235224213 38-39 0.019901106672925297 40-41 0.009312227027457893 42-43 0.00598999538702854 44-45 5.429442168546203E-4 46-47 0.007533717863058433 48-49 0.008998199831763599 50-51 0.011073127190229634 52-53 0.013602953757411702 54-55 0.026372414771111446 56-57 0.03280263519883292 58-59 0.023569648678699758 60-61 0.027666676390748674 62-63 0.01573364295436551 64-65 0.02730275702377585 66-67 0.02967116774270384 68-69 0.03246806416790629 70-71 0.03504484807276227 72-73 0.03777424332505847 74-75 0.03688205390925412 76-77 0.047755612414369625 78-79 0.017256821595163067 80-81 0.013376971569855995 82-83 0.016470286189125022 84-85 0.019733821157461983 86-87 0.03183707494291309 88-89 0.03828490437226228 90-91 0.03193392445186553 92-93 0.02773711239725955 94-95 0.031100431708153572 96-97 0.032236212313141346 98-99 0.020514486896290787 100-101 0.243045310132965 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.7036741E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.213330891171854 #Duplication Level Percentage of deduplicated Percentage of total 1 77.56101507040427 15.677664618732482 2 10.308061457255132 4.167205141640662 3 3.3464921689150677 2.029312606049869 4 1.764162682557725 1.4263841619358666 5 0.9990541167599858 1.0097105720128516 6 0.8599227838214313 1.042914226214414 7 0.6286225842307139 0.8894589410503279 8 0.3532093951294045 0.5711630702097055 9 0.29592955439707463 0.5383549803154599 >10 2.6750355602145577 11.469113460301909 >50 0.4980278612599049 7.222313923746264 >100 0.5962455071909971 24.709182537159442 >500 0.06767560710213412 9.165490977710034 >1k 0.04506848304079964 18.054333512048203 >5k 0.0014192296131478204 1.8045536550026917 >10k+ 5.793810791650368E-5 0.22284361586958176 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTGTGTTTGAGGGGTCCATCCCGGATTACCAATCTCAGATAAACTCCG 23934 0.14048461498592954 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.3500234581250017E-4 0.0 0.0 2 1.4674168023097846E-4 0.0 0.0 3 1.5261134744021758E-4 0.0 0.0 4 1.5848101464945673E-4 0.0 0.0 5 1.6435068185869587E-4 0.0 0.0 6 1.6435068185869587E-4 0.0 0.0 7 1.70220349067935E-4 0.0 0.0 8 1.878293506956524E-4 0.0 0.0 9 1.9369901790489155E-4 0.0 0.0 10-11 2.171776867418481E-4 0.0 0.0 12-13 2.729395252296199E-4 0.0 0.0 14-15 3.521800325543483E-4 0.0 0.0 16-17 4.783778775529898E-4 0.0 0.0 18-19 5.634880520869573E-4 0.0 0.0 20-21 7.395780683641314E-4 0.0 0.0 22-23 9.684950895244577E-4 0.0 0.0 24-25 0.0011798031090570669 0.0 0.0 26-27 0.0014556774678913063 0.0 0.0 28-29 0.0017344866603301653 0.0 0.0 30-31 0.0021072105281168506 0.0 0.0 32-33 0.0025562400696236445 0.0 0.0 34-35 0.0031167932881059823 0.0 0.0 36-37 0.003938546697399462 5.8696672092391376E-6 0.0 38-39 0.00504204413273642 5.8696672092391376E-6 0.0 40-41 0.006424350760512237 5.8696672092391376E-6 0.0 42-43 0.008270361097817945 5.8696672092391376E-6 0.0 44-45 0.010656380818373654 5.8696672092391376E-6 0.0 46-47 0.013814261776944312 5.8696672092391376E-6 0.0 48-49 0.017582588125275838 5.8696672092391376E-6 0.0 50-51 0.022231364554993235 5.8696672092391376E-6 0.0 52-53 0.028083422762604657 5.8696672092391376E-6 0.0 54-55 0.03592236332054352 5.8696672092391376E-6 2.9348336046195688E-6 56-57 0.04551046470683566 5.8696672092391376E-6 5.8696672092391376E-6 58-59 0.057323169965429424 5.8696672092391376E-6 5.8696672092391376E-6 60-61 0.07204136049259656 5.8696672092391376E-6 5.8696672092391376E-6 62-63 0.09070690221797702 5.8696672092391376E-6 5.8696672092391376E-6 64-65 0.11390676186249471 5.8696672092391376E-6 5.8696672092391376E-6 66-67 0.14287356954008984 5.8696672092391376E-6 5.8696672092391376E-6 68-69 0.1781561391348263 5.8696672092391376E-6 5.8696672092391376E-6 70-71 0.22231951521714158 5.8696672092391376E-6 5.8696672092391376E-6 72-73 0.27710992378178434 5.8696672092391376E-6 5.8696672092391376E-6 74-75 0.3459464459781363 5.8696672092391376E-6 5.8696672092391376E-6 76-77 0.42898169315363777 5.8696672092391376E-6 5.8696672092391376E-6 78-79 0.5316304333088118 5.8696672092391376E-6 5.8696672092391376E-6 80-81 0.6554246495852698 5.8696672092391376E-6 5.8696672092391376E-6 82-83 0.8062545530274834 5.8696672092391376E-6 5.8696672092391376E-6 84-85 0.9888628347405175 5.8696672092391376E-6 5.8696672092391376E-6 86-87 1.2035253690832066 5.8696672092391376E-6 5.8696672092391376E-6 88-89 1.4582806652986036 5.8696672092391376E-6 5.8696672092391376E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 143125 0.0 66.80066 1 TTTGTGT 154605 0.0 61.972088 2 AACTTTG 16800 0.0 59.548073 2 TAACTTT 17130 0.0 58.42208 1 ACTTTGT 41925 0.0 56.336174 1 TTGTGTT 174510 0.0 56.333843 3 TGTGTTT 184790 0.0 53.429832 4 GTGTTTG 199330 0.0 49.47277 5 TTTGAGG 63805 0.0 34.744194 8 GTTTGAG 166850 0.0 33.953136 7 TGTTTGA 290930 0.0 33.88483 6 TTGAGGG 34205 0.0 33.25196 9 TTTGAGC 44530 0.0 32.04471 8 TTGAGGC 10990 0.0 30.92683 9 TTTGACC 14575 0.0 28.758894 8 TGAGTCT 3970 0.0 28.09934 5 TTGAGGT 18265 0.0 27.808922 9 GTTTGAC 56640 0.0 27.623838 7 TTTGATG 29960 0.0 27.553566 8 TTTGAGT 64790 0.0 26.73405 3 >>END_MODULE