FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l02n01_6617.34100000035d2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l02n01_6617.34100000035d2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17036741
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGGGGTCCATCCCGGATTACCAATCTCAGATAAACTCCG239340.14048461498592954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1431250.066.800661
TTTGTGT1546050.061.9720882
AACTTTG168000.059.5480732
TAACTTT171300.058.422081
ACTTTGT419250.056.3361741
TTGTGTT1745100.056.3338433
TGTGTTT1847900.053.4298324
GTGTTTG1993300.049.472775
TTTGAGG638050.034.7441948
GTTTGAG1668500.033.9531367
TGTTTGA2909300.033.884836
TTGAGGG342050.033.251969
TTTGAGC445300.032.044718
TTGAGGC109900.030.926839
TTTGACC145750.028.7588948
TGAGTCT39700.028.099345
TTGAGGT182650.027.8089229
GTTTGAC566400.027.6238387
TTTGATG299600.027.5535668
TTTGAGT647900.026.734053
Produced by FastQC (version 0.11.2)