##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l02n01_6471.34100000035cd9.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13607858 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.75005713610474 33.0 33.0 33.0 33.0 33.0 2 32.76488746428718 33.0 33.0 33.0 33.0 33.0 3 32.79850774456935 33.0 33.0 33.0 33.0 33.0 4 36.56682344862799 37.0 37.0 37.0 37.0 37.0 5 36.64495639210815 37.0 37.0 37.0 37.0 37.0 6 36.670627221418684 37.0 37.0 37.0 37.0 37.0 7 36.65460442047529 37.0 37.0 37.0 37.0 37.0 8 36.65825334156191 37.0 37.0 37.0 37.0 37.0 9 36.63572452034699 37.0 37.0 37.0 37.0 37.0 10-11 36.63002817930639 37.0 37.0 37.0 37.0 37.0 12-13 36.613243612624416 37.0 37.0 37.0 37.0 37.0 14-15 38.73036538888046 40.0 37.0 40.0 37.0 40.0 16-17 38.70184341282809 40.0 37.0 40.0 37.0 40.0 18-19 38.674839273014165 40.0 37.0 40.0 37.0 40.0 20-21 38.63416483328971 40.0 37.0 40.0 37.0 40.0 22-23 38.58911733205917 40.0 37.0 40.0 37.0 40.0 24-25 38.54178651776054 40.0 37.0 40.0 37.0 40.0 26-27 38.471466927417964 40.0 37.0 40.0 37.0 40.0 28-29 38.404045919644375 40.0 37.0 40.0 37.0 40.0 30-31 38.35324369199032 40.0 37.0 40.0 37.0 40.0 32-33 38.2588005400997 40.0 37.0 40.0 37.0 40.0 34-35 38.191605320984394 40.0 37.0 40.0 37.0 40.0 36-37 38.10237066700725 40.0 37.0 40.0 37.0 40.0 38-39 38.01064587093722 40.0 37.0 40.0 37.0 40.0 40-41 37.902997922229936 40.0 37.0 40.0 33.0 40.0 42-43 37.777678309106406 40.0 37.0 40.0 33.0 40.0 44-45 37.650650932718435 40.0 37.0 40.0 33.0 40.0 46-47 37.463182192230406 40.0 37.0 40.0 33.0 40.0 48-49 37.22899930319673 37.0 37.0 40.0 33.0 40.0 50-51 37.02707564261767 37.0 37.0 40.0 33.0 40.0 52-53 36.805927942516746 37.0 37.0 40.0 33.0 40.0 54-55 36.56011776430942 37.0 37.0 40.0 33.0 40.0 56-57 36.27811739364124 37.0 37.0 40.0 33.0 40.0 58-59 35.98215670680867 37.0 37.0 38.5 33.0 40.0 60-61 35.701440667590745 37.0 35.0 37.0 33.0 40.0 62-63 35.43965317686296 37.0 33.0 37.0 33.0 40.0 64-65 35.18634207529209 37.0 33.0 37.0 33.0 40.0 66-67 34.89495135825197 37.0 33.0 37.0 33.0 38.5 68-69 34.65836122040662 37.0 33.0 37.0 27.0 37.0 70-71 34.408589801569065 37.0 33.0 37.0 27.0 37.0 72-73 34.221952786397395 37.0 33.0 37.0 27.0 37.0 74-75 34.00446844756904 37.0 33.0 37.0 27.0 37.0 76-77 31.01116072051898 33.0 30.0 33.0 24.5 37.0 78-79 33.73695849118943 35.0 33.0 37.0 27.0 37.0 80-81 34.23276852242285 37.0 33.0 37.0 27.0 37.0 82-83 34.23134309602584 37.0 33.0 37.0 27.0 37.0 84-85 34.16852894849431 37.0 33.0 37.0 27.0 37.0 86-87 34.04261820633343 37.0 33.0 37.0 27.0 37.0 88-89 33.90655057541018 37.0 33.0 37.0 27.0 37.0 90-91 33.82892245054292 37.0 33.0 37.0 27.0 37.0 92-93 33.7393911664863 37.0 33.0 37.0 27.0 37.0 94-95 33.63577166222634 37.0 33.0 37.0 27.0 37.0 96-97 33.495548307455884 37.0 33.0 37.0 27.0 37.0 98-99 33.33657530817855 37.0 33.0 37.0 27.0 37.0 100-101 31.322961740194526 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.07174084629981081 2306 2 0.06733130547863198 2306 3 0.05303083504605155 2306 4 -0.18731078298944226 2306 5 -0.09297248267319702 2306 6 0.00390863709581879 2306 7 0.044251560351220576 2306 8 0.08319022799781806 2306 9 0.0835759097169202 2306 10-11 0.09426167912700834 2306 12-13 0.06103240940942811 2306 14-15 0.13044845432270336 2306 16-17 0.110866657683232 2306 18-19 0.16937938678362485 2306 20-21 0.12433104194001743 2306 22-23 0.17452492798142316 2306 24-25 0.08254171855971038 2306 26-27 0.06310786078957875 2306 28-29 0.12091524737068937 2306 30-31 0.09459759131553369 2306 32-33 0.16263432966107416 2306 34-35 0.16375709138232253 2306 36-37 0.13496921477246815 2306 38-39 0.028530748424543617 2306 40-41 0.05208265385584099 2306 42-43 0.06869322827647295 2306 44-45 -0.058444146457468094 2306 46-47 -0.02788156528693264 2306 48-49 0.03185216573140792 2306 50-51 0.009341305145547096 2306 52-53 -0.03375157907048987 2306 54-55 -0.05113143453938562 2306 56-57 -0.05296248441751317 2306 58-59 -0.059199642258903395 2306 60-61 -0.05355754194619777 2306 62-63 -0.06981206197634293 2306 64-65 -0.1566167330441388 2306 66-67 -0.19289681114467072 2306 68-69 -0.20972050883565174 2306 70-71 -0.2927038307012211 2306 72-73 -0.3440186955681739 2306 74-75 -0.45668073558058353 2306 76-77 -0.4888488375356488 2306 78-79 -0.27647260405726826 2306 80-81 -0.20347803642594897 2306 82-83 -0.22468290550575176 2306 84-85 -0.23593565014426332 2306 86-87 -0.213717894291797 2306 88-89 -0.31031246095488996 2306 90-91 -0.37462636061446375 2306 92-93 -0.3932601656179173 2306 94-95 -0.2970638867388189 2306 96-97 -0.2664125435346065 2306 98-99 -0.26921955790660235 2306 100-101 -0.6867542615488489 2307 1 0.09909352032767771 2307 2 0.06692285765169004 2307 3 0.06924016571730363 2307 4 -0.08161033588170596 2307 5 -0.03436645424535101 2307 6 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-0.22447322490302923 1213 42-43 -0.16634419737236072 1213 44-45 -0.13587483043657045 1213 46-47 -0.1194871130974775 1213 48-49 -0.06396061068687686 1213 50-51 -0.09677777365401852 1213 52-53 -0.0510114693587127 1213 54-55 0.016068332496537607 1213 56-57 -0.03430291539240926 1213 58-59 -0.028184445602668973 1213 60-61 -0.00735092469128773 1213 62-63 -0.001337990167968428 1213 64-65 0.07171147515312981 1213 66-67 0.061387165896810814 1213 68-69 0.07533156360764082 1213 70-71 0.07857277650020933 1213 72-73 0.09353241376364707 1213 74-75 0.19343213584354402 1213 76-77 0.5528667018599016 1213 78-79 -0.016823551637322964 1213 80-81 -0.0051146816912677195 1213 82-83 0.004205722269183809 1213 84-85 0.08073826740281476 1213 86-87 0.0806586957796398 1213 88-89 0.1637358904381685 1213 90-91 0.15390648443149502 1213 92-93 0.15458397431572735 1213 94-95 0.23271815463249368 1213 96-97 0.15757270140051816 1213 98-99 0.3164649829775712 1213 100-101 1.016863727455167 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 3.0 7 33.0 8 337.0 9 969.0 10 666.0 11 237.0 12 302.0 13 542.0 14 1083.0 15 2354.0 16 4022.0 17 5756.0 18 8151.0 19 10490.0 20 14096.0 21 18120.0 22 23298.0 23 29984.0 24 38312.0 25 49569.0 26 64073.0 27 81896.0 28 106137.0 29 137317.0 30 181492.0 31 241875.0 32 332098.0 33 482314.0 34 773369.0 35 1509268.0 36 3506390.0 37 5079888.0 38 903400.0 39 17.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 20.381341793042964 22.726637600211028 30.658159865996552 26.23386074074946 2 27.133587078877515 22.51118434657387 29.737633946503557 20.61759462804506 3 21.435473954967478 20.752353751002914 37.037772451115366 20.774399842914246 4 21.469450528310745 20.727073961435572 36.074241202719485 21.729234307534202 5 28.34487249940439 20.006308119911303 31.528121472167037 20.120697908517272 6 29.623993724802244 20.61827805669342 30.313242539714917 19.444485678789416 7 28.30682830464574 26.083870069778797 25.60119307535396 20.008108550221497 8 27.130816620808357 21.918196089347788 29.97759088902897 20.97339640081488 9 23.050255227531032 21.174155403444097 34.18057419470426 21.595015174320604 10-11 27.43715432656631 21.993733326729306 28.41645613879863 22.152656207905753 12-13 26.63896788147577 25.742931434189625 24.66017877812437 22.95792190621023 14-15 26.413337793501373 23.87868465411676 25.945141402857086 23.762836149524784 16-17 25.773725739936438 23.506377712054313 25.5368883184995 25.183008229509745 18-19 25.720807786207057 24.180745419301115 26.025778634668296 24.072668159823536 20-21 24.63834334209263 24.633500542206196 26.67937038163431 24.048785734066865 22-23 24.805749459581797 24.789953312261762 25.818495984107876 24.585801244048568 24-25 25.389760093028602 24.749578515590038 25.570442460525385 24.290218930855982 26-27 25.199392174637946 24.61546844705 25.926784498601396 24.258354879710662 28-29 25.117538282546104 25.201912682361854 25.31042029900424 24.370128736087803 30-31 25.258030147167844 25.01476712586993 25.303403576777022 24.423799150185207 32-33 25.028842030991942 25.54536213858263 25.0959824062233 24.32981342420213 34-35 24.761526642497294 25.41851269994343 25.434996326458847 24.384964331100427 36-37 24.98442552848369 25.327381804452703 25.329178964873083 24.359013702190527 38-39 24.9230559808326 25.040015079546702 25.53882128822445 24.49810765139625 40-41 24.72299352964096 25.34151015759904 25.022425720073144 24.913070592686857 42-43 24.63192698766041 25.19280312397686 25.214846900661165 24.960422987701563 44-45 24.949957893605138 25.0751839543679 25.190963220663924 24.783894931363033 46-47 24.5782170222431 25.031617348306824 25.33238131280867 25.057784316641406 48-49 24.61026358322912 24.74636633077164 25.219789878935504 25.423580207063733 50-51 24.74838953870803 24.897386146909348 25.63432882440919 24.719895489973432 52-53 24.644914687065864 25.119667564945118 25.660181745941102 24.575236002047916 54-55 24.64967847369784 25.057543587933257 25.08105094344581 25.211726994923094 56-57 24.745173567783233 25.480643732575686 25.354437009661133 24.419745689979948 58-59 24.53427159845358 25.82113358241667 25.420587696876595 24.22400712225316 60-61 25.241196779715825 25.46298586584776 24.869776783407346 24.42604057102907 62-63 24.628633228207207 25.75636843341005 25.48383233361291 24.13116600476983 64-65 24.919490787708508 25.417903464069173 25.245765302355228 24.416840445867095 66-67 25.205933725798772 25.454896692516126 25.406998418570414 23.93217116311468 68-69 24.857709224741136 24.966118968658172 26.066329929351912 24.10984187724878 70-71 24.79066560926188 25.456005815138237 25.502458407323736 24.25087016827615 72-73 24.45421136037858 25.14122446573111 25.972370461520413 24.4321937123699 74-75 24.604754974512403 25.156119386680377 25.875154038946572 24.363971599860648 76-77 24.500025916535524 25.058444544679553 25.685859974980435 24.75566956380449 78-79 24.68815407749665 25.111541149079454 25.227975533748552 24.972329239675346 80-81 24.595670777407577 24.902729655194968 25.732509261983065 24.769090305414384 82-83 24.346024432639428 25.666722146226213 25.61337283517172 24.373880585962638 84-85 24.62348192123741 25.318694557003614 25.350847727962638 24.70697579379634 86-87 24.31441595220018 25.40779106771677 25.70094215373168 24.57685082635137 88-89 24.342894652193717 26.707971430836736 24.807585195257253 24.141548721712297 90-91 24.45066118987346 25.691694708915247 24.957685774061993 24.8999583271493 92-93 24.33804867020943 25.931084208951333 24.96709920246048 24.76376791837875 94-95 24.244609036496957 26.27437784700339 24.606173298487338 24.874839818012312 96-97 24.28218090258955 25.64013145279479 24.821879669404552 25.255807975211113 98-99 24.613488833787024 25.53948683233242 24.726578736437535 25.12044559744302 100-101 24.596873878455682 26.331727984140297 24.265080922092856 24.806317215311168 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1984.0 1 1592.0 2 1345.0 3 1479.0 4 1533.5 5 1519.5 6 1419.5 7 1426.5 8 1489.0 9 1621.0 10 1684.0 11 1699.5 12 1737.5 13 1773.0 14 1846.0 15 1893.5 16 1995.5 17 2143.0 18 2251.5 19 2333.5 20 2472.5 21 2705.5 22 2921.5 23 3126.5 24 3428.0 25 3949.5 26 4555.5 27 5288.5 28 6859.5 29 8971.5 30 10737.5 31 12999.5 32 17403.0 33 23993.0 34 30853.0 35 41477.5 36 56328.5 37 79308.5 38 111105.5 39 149750.0 40 204776.0 41 262518.5 42 308567.5 43 365495.0 44 492162.5 45 663777.0 46 826340.0 47 955972.0 48 1023849.0 49 1019273.0 50 941402.5 51 865383.5 52 784924.5 53 714995.5 54 676596.5 55 636536.5 56 601223.5 57 493919.5 58 354643.5 59 256692.0 60 178208.0 61 124124.0 62 91245.5 63 66832.0 64 41289.5 65 21140.0 66 10456.0 67 4658.5 68 2731.0 69 1686.0 70 1180.0 71 865.5 72 645.5 73 481.0 74 352.0 75 254.5 76 164.5 77 117.5 78 90.0 79 65.0 80 52.5 81 40.5 82 31.5 83 23.5 84 18.0 85 12.0 86 10.0 87 7.0 88 4.0 89 4.0 90 3.0 91 2.0 92 2.0 93 1.0 94 0.5 95 0.0 96 1.0 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12612565475036555 2 0.0 3 2.9394780574576835E-5 4 0.526173920980069 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 3.0129650088941257E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.0208911645021576E-4 22-23 8.04682118229041E-4 24-25 0.0 26-27 1.4697390287288417E-5 28-29 4.2254997075954207E-4 30-31 0.005698913083896084 32-33 0.00401606189600156 34-35 0.0030533828321841687 36-37 0.02250537887741039 38-39 0.019654085161676438 40-41 0.009343865875143612 42-43 0.006194950006092067 44-45 4.960369221959841E-4 46-47 0.007411159052365185 48-49 0.009009500246107801 50-51 0.011081832276615466 52-53 0.013462809503156191 54-55 0.026495720340409198 56-57 0.03220933081459257 58-59 0.023328432733498543 60-61 0.027315099848925526 62-63 0.0157078358695395 64-65 0.026966036829602424 66-67 0.02959686969102705 68-69 0.03215788994858706 70-71 0.03484751237116084 72-73 0.03772452651989755 74-75 0.03650096877848079 76-77 0.04778856451911829 78-79 0.017203295331271094 80-81 0.013488529936158945 82-83 0.016508843640196717 84-85 0.019606318643242752 86-87 0.031544273904092764 88-89 0.03784210564219585 90-91 0.03143036912936628 92-93 0.02722324115962997 94-95 0.03079470699944106 96-97 0.031919057356418624 98-99 0.02023095773045251 100-101 0.24122826678526482 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.3607858E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.40294611455521 #Duplication Level Percentage of deduplicated Percentage of total 1 75.32884700458715 12.356150182878164 2 11.003046193347608 3.6096474761088517 3 3.5577368243162546 1.7507209625708484 4 1.6015201799835184 1.0507859685456966 5 1.2015441823385975 0.9854432238578653 6 0.8458154846246587 0.8324319490292805 7 0.6870585992814959 0.7888849627089309 8 0.3675090429384907 0.4822584822345454 9 0.39362683767579154 0.58109758268749 >10 3.3951228664300843 11.599367274037336 >50 0.5949787062922036 6.913137319614126 >100 0.8597396476623118 30.843979887865462 >500 0.1059958038185276 11.904700305749651 >1k 0.056409624697612994 15.162879197569556 >5k 9.592074347130662E-4 0.9861894083182224 >10k+ 8.979457108547452E-5 0.15232581622413277 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.818434172373051E-5 0.0 0.0 2 9.553303686737472E-5 0.0 0.0 3 9.553303686737472E-5 0.0 0.0 4 1.0288173201101893E-4 0.0 0.0 5 1.1023042715466314E-4 0.0 0.0 6 1.1023042715466314E-4 0.0 0.0 7 1.1757912229830734E-4 0.0 0.0 8 1.3962520772923997E-4 0.0 0.0 9 1.616712931601726E-4 0.0 0.0 10-11 2.0943781159385997E-4 0.0 0.0 12-13 3.086451960330568E-4 0.0 0.0 14-15 4.22549970759542E-4 0.0 0.0 16-17 6.136160444942915E-4 0.0 0.0 18-19 8.487742890909062E-4 0.0 0.0 20-21 0.00111700166183392 0.0 0.0 22-23 0.001403600772436044 0.0 0.0 24-25 0.00169387423060999 0.0 0.0 26-27 0.00211274985379771 0.0 0.0 28-29 0.0025463228672727182 0.0 0.0 30-31 0.003035011094325058 0.0 0.0 32-33 0.0036376040961038835 7.348695143644209E-6 0.0 34-35 0.004464332299763857 7.348695143644209E-6 0.0 36-37 0.005434360058724892 7.348695143644209E-6 0.0 38-39 0.006635871714710721 7.348695143644209E-6 0.0 40-41 0.008495091586052706 7.348695143644209E-6 0.0 42-43 0.010600492744706771 7.348695143644209E-6 0.0 44-45 0.013117420831404913 7.348695143644209E-6 0.0 46-47 0.016530889725627648 7.348695143644209E-6 0.0 48-49 0.0208445737749468 7.348695143644209E-6 0.0 50-51 0.02570206126489562 7.348695143644209E-6 0.0 52-53 0.0322313769000235 7.348695143644209E-6 0.0 54-55 0.04009080635615098 7.348695143644209E-6 0.0 56-57 0.049603692219598414 7.348695143644209E-6 0.0 58-59 0.06143509140086559 7.348695143644209E-6 0.0 60-61 0.07555928346694976 7.348695143644209E-6 0.0 62-63 0.09304550356125116 7.348695143644209E-6 0.0 64-65 0.11457350598455687 7.348695143644209E-6 0.0 66-67 0.14025719551159338 7.348695143644209E-6 0.0 68-69 0.17172063376910607 7.348695143644209E-6 0.0 70-71 0.21033802674895638 7.348695143644209E-6 0.0 72-73 0.2577187386876024 7.348695143644209E-6 0.0 74-75 0.3148621921245798 7.348695143644209E-6 0.0 76-77 0.3840354595117027 7.348695143644209E-6 0.0 78-79 0.46808616021713334 7.348695143644209E-6 0.0 80-81 0.5679108350483963 7.348695143644209E-6 0.0 82-83 0.686474682495952 7.348695143644209E-6 0.0 84-85 0.8281318044324095 7.348695143644209E-6 0.0 86-87 0.9941204559894732 7.348695143644209E-6 0.0 88-89 1.1895626776822628 7.348695143644209E-6 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 123920 0.0 63.98204 1 AACTTTG 16270 0.0 60.47911 2 TTTGTGT 132235 0.0 60.08944 2 TAACTTT 16440 0.0 59.4928 1 ACTTTGT 39090 0.0 58.01101 1 TTGTGTT 147950 0.0 55.08733 3 TGTGTTT 157725 0.0 51.89113 4 GTGTTTG 175840 0.0 46.532627 5 TTTGAGG 54755 0.0 33.561623 8 TTGAGGG 26215 0.0 32.63236 9 TGAGTCT 3145 0.0 32.604073 5 GTTTGAG 143895 0.0 32.298367 7 GTTTGAA 16340 0.0 32.24195 2 TGTTTGA 253835 0.0 32.22197 6 TTGAGGC 9450 0.0 31.497732 9 TTGACGC 2710 0.0 30.4806 9 TTTGACC 12775 0.0 30.248726 8 TTTGAGT 53330 0.0 29.144087 3 TTTGAGC 35555 0.0 29.040369 8 TTGATCT 4720 0.0 28.91534 4 >>END_MODULE