FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l02n01_6471.34100000035cd9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l02n01_6471.34100000035cd9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13607858
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1239200.063.982041
AACTTTG162700.060.479112
TTTGTGT1322350.060.089442
TAACTTT164400.059.49281
ACTTTGT390900.058.011011
TTGTGTT1479500.055.087333
TGTGTTT1577250.051.891134
GTGTTTG1758400.046.5326275
TTTGAGG547550.033.5616238
TTGAGGG262150.032.632369
TGAGTCT31450.032.6040735
GTTTGAG1438950.032.2983677
GTTTGAA163400.032.241952
TGTTTGA2538350.032.221976
TTGAGGC94500.031.4977329
TTGACGC27100.030.48069
TTTGACC127750.030.2487268
TTTGAGT533300.029.1440873
TTTGAGC355550.029.0403698
TTGATCT47200.028.915344
Produced by FastQC (version 0.11.2)