FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l02n01_6271.34100000035c96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l02n01_6271.34100000035c96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16598639
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1324250.064.103371
TTTGTGT1422250.059.8280562
ACTTTGT398250.056.0302161
TTGTGTT1577050.055.810463
TGTGTTT1720150.051.508814
TAACTTT171150.051.062021
AACTTTG180900.048.6992872
GTGTTTG1892150.046.8036735
GTTTGAG1587750.032.8404122
TTGAGTC198350.032.8179634
TGTTTGA2903850.032.805511
TTTGAGT614950.031.530913
TTTGAGG587250.031.4853068
TTGAGGG284250.030.6616619
GTTTGAC541000.026.5087
TTTGAGC445200.025.835798
TTGAGGT196900.025.7642214
CTTGTGT107200.025.731352
TTGAGTG276900.025.6971044
TTGAGGC107550.024.9376414