FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l02n01_6205.34100000035c53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l02n01_6205.34100000035c53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16052683
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTT238870.14880378563508667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT395500.053.5057221
CTTTGTG958850.052.6602861
TTTGTGT1060300.047.7116472
TTGTGTT1144050.045.603763
TGTGTTT1267950.041.369434
GTGTTTG1430650.036.6955155
GTTTGAG1137400.029.0250972
TGTTTGA1959200.028.1867491
TTTGAGG533550.027.4133243
TTGAGGG380950.026.2309284
TTTGAGT392000.025.6255043
TTGAGTC126650.025.5087074
GTTTGAA132800.024.7946132
TAACTTT391550.023.8683661
AACTTTG396450.023.8573152
TGAGGGG314200.023.2370325
GTTTGAC398600.023.03317
TTTGAGA158850.021.4194643
TTGAGTG191950.020.1870524
GTTTGAT562650.020.1348652
Produced by FastQC (version 0.11.2)