FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l02n01_6019.34100000035bf4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l02n01_6019.34100000035bf4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15647248
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1395000.062.1470031
TTTGTGT1509450.057.6262932
ACTTTGT455000.054.4095881
TTGTGTT1729500.051.6687553
TGTGTTT1894950.047.4093974
TAACTTT247150.046.390251
AACTTTG258750.044.5877762
GTGTTTG2264000.039.712585
TTGAGTC182350.031.8569624
TTTGAGG660550.030.909348
TTTGAGT616150.030.670343
GTTTGAG1656500.029.8904482
GTTTGAA184650.029.7540112
TGTTTGA3016250.029.7517386
GTTTGAC593700.028.6327617
TTGAGGG413550.026.4128069
TTTGACC181700.026.3610218
TTGAGTG357300.024.327444
TTGACCC110550.024.0896269
TGAGTCC136450.023.8309635