##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l02n01_3522.34100000035b7d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15190797 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74339555719163 33.0 33.0 33.0 33.0 33.0 2 32.756431607900495 33.0 33.0 33.0 33.0 33.0 3 32.79044575475533 33.0 33.0 33.0 33.0 33.0 4 36.55581132444861 37.0 37.0 37.0 37.0 37.0 5 36.63658128010005 37.0 37.0 37.0 37.0 37.0 6 36.66014423074708 37.0 37.0 37.0 37.0 37.0 7 36.64260170154338 37.0 37.0 37.0 37.0 37.0 8 36.64430108571657 37.0 37.0 37.0 37.0 37.0 9 36.623336418754064 37.0 37.0 37.0 37.0 37.0 10-11 36.61632628623765 37.0 37.0 37.0 37.0 37.0 12-13 36.5958493817013 37.0 37.0 37.0 37.0 37.0 14-15 38.6859279338668 40.0 37.0 40.0 37.0 40.0 16-17 38.6634089376614 40.0 37.0 40.0 37.0 40.0 18-19 38.625465701371695 40.0 37.0 40.0 37.0 40.0 20-21 38.589370360225345 40.0 37.0 40.0 37.0 40.0 22-23 38.55149670553823 40.0 37.0 40.0 37.0 40.0 24-25 38.50551044161804 40.0 37.0 40.0 37.0 40.0 26-27 38.42841530961147 40.0 37.0 40.0 37.0 40.0 28-29 38.357538646589774 40.0 37.0 40.0 37.0 40.0 30-31 38.29251819374586 40.0 37.0 40.0 37.0 40.0 32-33 38.183539382430034 40.0 37.0 40.0 37.0 40.0 34-35 38.10346718476983 40.0 37.0 40.0 37.0 40.0 36-37 38.01016009890725 40.0 37.0 40.0 37.0 40.0 38-39 37.90330787120649 40.0 37.0 40.0 33.0 40.0 40-41 37.771432137497456 40.0 37.0 40.0 33.0 40.0 42-43 37.62148901733069 40.0 37.0 40.0 33.0 40.0 44-45 37.45102692768523 40.0 37.0 40.0 33.0 40.0 46-47 37.22446244920526 37.0 37.0 40.0 33.0 40.0 48-49 36.950897276818324 37.0 37.0 40.0 33.0 40.0 50-51 36.70411898072234 37.0 37.0 40.0 33.0 40.0 52-53 36.46278654767094 37.0 37.0 40.0 33.0 40.0 54-55 36.20620596799496 37.0 37.0 40.0 33.0 40.0 56-57 35.913367810787015 37.0 37.0 38.5 33.0 40.0 58-59 35.61877612478134 37.0 33.0 37.0 33.0 40.0 60-61 35.3318646809644 37.0 33.0 37.0 33.0 40.0 62-63 35.07511923172958 37.0 33.0 37.0 33.0 40.0 64-65 34.82317708544193 37.0 33.0 37.0 33.0 37.0 66-67 34.540138381152744 37.0 33.0 37.0 27.0 37.0 68-69 34.32979168242456 37.0 33.0 37.0 27.0 37.0 70-71 34.103841918235105 37.0 33.0 37.0 27.0 37.0 72-73 33.924032524429094 37.0 33.0 37.0 27.0 37.0 74-75 33.71997170392047 37.0 33.0 37.0 27.0 37.0 76-77 30.664279629304506 33.0 30.0 33.0 24.5 35.0 78-79 33.49337174343124 35.0 33.0 37.0 27.0 37.0 80-81 34.048927551332554 37.0 33.0 37.0 27.0 37.0 82-83 34.06456623046177 37.0 33.0 37.0 27.0 37.0 84-85 33.99863930773349 37.0 33.0 37.0 27.0 37.0 86-87 33.88105370639868 37.0 33.0 37.0 27.0 37.0 88-89 33.72941495433057 37.0 33.0 37.0 27.0 37.0 90-91 33.658320099992125 37.0 33.0 37.0 27.0 37.0 92-93 33.565198093292935 37.0 33.0 37.0 27.0 37.0 94-95 33.45520067182782 37.0 33.0 37.0 27.0 37.0 96-97 33.30429213161101 37.0 33.0 37.0 27.0 37.0 98-99 33.138689069441185 35.0 33.0 37.0 27.0 37.0 100-101 31.090728616806608 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.08513868316018147 2306 2 0.07926334795600098 2306 3 0.07849471096864846 2306 4 -0.0717555756126842 2306 5 -0.042247954022528234 2306 6 0.03158347451807941 2306 7 0.06953733465379486 2306 8 0.05778585504025813 2306 9 0.09479953790783213 2306 10-11 0.10836965568715584 2306 12-13 0.1202213823343996 2306 14-15 0.20229881597529698 2306 16-17 0.1786252753798223 2306 18-19 0.17793120364516568 2306 20-21 0.15517771072985198 2306 22-23 0.20464787135720286 2306 24-25 0.10838912239049847 2306 26-27 0.1308553184495551 2306 28-29 0.1334898459966638 2306 30-31 0.09194635241644988 2306 32-33 0.22708728068403872 2306 34-35 0.20870015685547827 2306 36-37 0.14712929702567834 2306 38-39 0.045386980075853955 2306 40-41 0.11229441134247509 2306 42-43 0.14274924232592667 2306 44-45 0.027004066582740904 2306 46-47 -0.03417735231380448 2306 48-49 0.0653854938746079 2306 50-51 0.04037265280021529 2306 52-53 0.04247352928041437 2306 54-55 0.02588263591435691 2306 56-57 0.03835527355328594 2306 58-59 -0.033478198350245236 2306 60-61 -0.09383330546685897 2306 62-63 -0.022747634711954845 2306 64-65 -0.16047644663118632 2306 66-67 -0.059633824069607044 2306 68-69 -0.11896070302094586 2306 70-71 -0.28390302423266434 2306 72-73 -0.2001748124309941 2306 74-75 -0.3479577090100463 2306 76-77 -0.44104877153442956 2306 78-79 -0.19172382083542772 2306 80-81 -0.13092143785572574 2306 82-83 -0.08773273275970439 2306 84-85 -0.10281293253638069 2306 86-87 -0.0703848913117966 2306 88-89 -0.3011476780931446 2306 90-91 -0.2104651948189442 2306 92-93 -0.2415896154389614 2306 94-95 -0.2861644538031456 2306 96-97 -0.24970500079466973 2306 98-99 -0.1722923021370235 2306 100-101 -0.6171424967887837 2307 1 0.08987418092061006 2307 2 0.0818775796476956 2307 3 0.07572611212601998 2307 4 -0.11012545022618525 2307 5 -0.05912573252093978 2307 6 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-0.07446874865807018 1213 58-59 -0.09132774308027791 1213 60-61 -0.10412211668048599 1213 62-63 -0.04279629946680785 1213 64-65 0.06220160279438147 1213 66-67 0.05740433653758714 1213 68-69 0.058146464299511536 1213 70-71 0.09009034296278173 1213 72-73 0.0834936992069828 1213 74-75 0.18143522176112725 1213 76-77 0.5186078432032204 1213 78-79 -0.03440760757002437 1213 80-81 -0.08938704215574234 1213 82-83 -0.029303032805103157 1213 84-85 0.08319133636518927 1213 86-87 0.06656311385572167 1213 88-89 0.1374828324678674 1213 90-91 0.04767360120809627 1213 92-93 0.09756621839790824 1213 94-95 0.1105196662080914 1213 96-97 0.02578675607878722 1213 98-99 0.1971511931029113 1213 100-101 0.9812310559116959 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 44.0 8 410.0 9 1130.0 10 774.0 11 278.0 12 333.0 13 613.0 14 1181.0 15 2493.0 16 4393.0 17 6355.0 18 8886.0 19 12190.0 20 16156.0 21 21241.0 22 27740.0 23 36007.0 24 47002.0 25 60647.0 26 78348.0 27 102017.0 28 131681.0 29 169907.0 30 221082.0 31 294516.0 32 404241.0 33 595728.0 34 971081.0 35 1876436.0 36 4165564.0 37 5329381.0 38 602901.0 39 39.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 21.079279040209045 21.479545673708014 30.488267157116223 26.95290812896672 2 28.16656031938285 21.232408016511574 30.39396813741899 20.207063526686586 3 22.178385719241724 19.66095245869372 37.583311993648785 20.57734982841577 4 21.63659179002624 19.651354105704936 37.19301842572757 21.519035678541247 5 29.232337184151696 19.056926374567443 31.734352055392485 19.976384385888377 6 30.445696825518766 19.584337806633844 30.52415880483427 19.445806563013118 7 29.596472127170152 25.875383628653587 24.55850736468929 19.96963687948697 8 27.434478915095767 21.32326565880645 30.199468796798485 21.042786629299307 9 23.444859410602355 20.211987560626348 34.31555302858698 22.02760000018432 10-11 28.450913405004357 21.063628195413315 27.76608100285982 22.719377396722503 12-13 27.687574294813338 25.088679803281572 23.876708502988414 23.347037398916676 14-15 27.185709874208708 22.825079553100473 25.538696225089442 24.45051434760138 16-17 26.710685423549535 22.625211830557674 24.821900391401453 25.842202354491338 18-19 26.647357607372413 23.306403870711982 25.478462387457352 24.567776134458253 20-21 25.270052179060336 24.09191227197993 25.734011604943213 24.904023944016522 22-23 25.453215392595986 23.973811573938757 25.33003130867084 25.242941724794417 24-25 25.61356721441278 23.753036131020643 25.1868911157196 25.446505538846974 26-27 25.47273452173516 23.887787691933276 25.282546958458752 25.356930827872816 28-29 25.687019184656855 24.212758528505503 24.65322114475977 25.44700114207787 30-31 26.03882460225691 23.94683697569631 24.529521349502264 25.484817072544512 32-33 25.91727292417014 24.358135085867918 24.493515222653567 25.231076767308373 34-35 25.902688653921924 24.472961883937234 24.248641321789574 25.37570814035127 36-37 25.867116125751533 24.3297406060705 24.501135510584042 25.302007757593927 38-39 25.841622477249395 23.949651290042155 24.59469705923233 25.614029173476116 40-41 25.720558992224202 24.409668590281605 24.238604884909485 25.63116753258471 42-43 25.597170638554665 24.318096840730938 24.51649236890521 25.568240151809185 44-45 25.995747336028685 24.055318402259378 24.692072561305302 25.256861700406635 46-47 25.46004207267037 24.368155319761545 24.613532597695258 25.558270009872828 48-49 25.681773645912383 24.165744180651867 24.551126024356133 25.601356149079624 50-51 25.477862833436443 24.342518690493254 25.09988222505568 25.079736251014623 52-53 25.50299887551838 24.331765629706908 25.036562359055054 25.128673135719655 54-55 25.36608335031075 24.199017149187434 24.672933977979095 25.761965522522722 56-57 25.49045870399101 24.278481643651904 24.826123722331737 25.404935930025346 58-59 25.221756776687982 24.541957917636772 25.020352450985555 25.215932854689687 60-61 25.83694346725028 24.379885154595314 24.314970072458163 25.46820130569624 62-63 25.27313565069871 24.712608469207513 24.839257174661345 25.174998705432433 64-65 25.369237193324885 24.835069943723887 24.759027015306334 25.036665847644894 66-67 25.731259933234963 24.75559698445819 24.486484347302508 25.026658735004336 68-69 25.611334001444614 24.79794506724014 24.769478232485472 24.821242698829778 70-71 25.36412672687755 25.09015482775666 24.483464900801454 25.062253544564335 72-73 25.67856446104317 24.62721442453567 24.51825004383369 25.175971070587472 74-75 25.36049209824448 24.531782881517362 24.55972415124689 25.548000868991267 76-77 25.47904012375336 24.310293854304362 24.553929335897216 25.65673668604506 78-79 25.70420978457192 24.190239854861463 24.551147194566063 25.554403166000554 80-81 25.306654031022113 24.26890431870161 24.753959798477535 25.670481851798744 82-83 25.137121543814832 24.90111170732831 24.618194208060785 25.34357254079607 84-85 25.42739017866681 24.770844831653076 24.3481467081624 25.453618281517716 86-87 25.009490561163627 25.03741733992856 24.67377327788541 25.279318821022407 88-89 25.300726389955713 25.788496349611258 23.902305213381002 25.00847204705203 90-91 25.244800756793524 25.07261126591015 24.026544798817532 25.656043178478793 92-93 24.90309979259563 25.566473766158648 24.05037665050039 25.480049790745333 94-95 25.287751642552454 25.62056206944258 23.54602409689329 25.545662191111667 96-97 25.08918847427506 25.080789345571898 23.866349745250574 25.963672434902474 98-99 25.47304120200828 25.07171949777768 24.13538668546507 25.31985261474897 100-101 25.960030552174562 25.620094881468113 23.224954985563144 25.194919580794178 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 818.0 1 887.5 2 1073.0 3 1186.0 4 1309.5 5 1394.5 6 1331.0 7 1282.5 8 1313.5 9 1336.0 10 1292.5 11 1296.5 12 1388.0 13 1506.0 14 1579.5 15 1678.0 16 1822.0 17 1953.0 18 2064.0 19 2263.5 20 2488.5 21 2686.0 22 3041.0 23 3453.0 24 3771.0 25 4238.0 26 4882.0 27 5547.0 28 6218.0 29 6870.5 30 7912.5 31 9654.0 32 13495.0 33 19327.5 34 26249.5 35 39095.5 36 54414.0 37 76339.5 38 107114.5 39 127667.0 40 146382.5 41 174055.5 42 210876.5 43 266340.5 44 349769.0 45 455457.5 46 589665.5 47 761513.0 48 960926.5 49 1082148.5 50 1084861.0 51 1074314.5 52 1083762.0 53 1081199.5 54 1011469.5 55 906578.5 56 829506.0 57 694267.5 58 527615.5 59 407171.5 60 318505.0 61 239675.0 62 155398.5 63 93866.5 64 51743.5 65 27197.5 66 15674.0 67 10216.0 68 7439.0 69 5286.0 70 3772.0 71 2778.5 72 2145.5 73 1722.5 74 1362.5 75 981.0 76 670.5 77 472.5 78 349.5 79 265.0 80 177.5 81 113.5 82 70.0 83 49.5 84 37.0 85 26.0 86 18.5 87 15.5 88 14.0 89 9.0 90 7.0 91 6.0 92 4.0 93 3.0 94 3.5 95 5.0 96 4.0 97 2.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12777473097691977 2 0.0 3 4.608053152181548E-5 4 0.5303605860837979 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 3.2585518718998086E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.90905059161807E-4 22-23 7.70203169721773E-4 24-25 0.0 26-27 2.304026576090774E-5 28-29 4.213077167708844E-4 30-31 0.005536246715692402 32-33 0.003995840376248856 34-35 0.003172973741930723 36-37 0.02209232339817325 38-39 0.019485481900653405 40-41 0.009390554030838539 42-43 0.006178082690460547 44-45 5.496749117245132E-4 46-47 0.0074156741084750195 48-49 0.008666431392638582 50-51 0.010914503037595722 52-53 0.013300816277118311 54-55 0.026035500309825748 56-57 0.03148946036208633 58-59 0.022625540977211398 60-61 0.026552260556177532 62-63 0.015321776731003645 64-65 0.026203365103226645 66-67 0.028997820193371025 68-69 0.030966117182660003 70-71 0.0336749941428353 72-73 0.036597816427933305 74-75 0.0353766823426052 76-77 0.046396512309393644 78-79 0.016783187873552652 80-81 0.013290941877506493 82-83 0.016108437233411783 84-85 0.019021385118897973 86-87 0.030650136395081837 88-89 0.036634022559843306 90-91 0.030416442270935488 92-93 0.026588466688087533 94-95 0.029781189229241885 96-97 0.030926619584212733 98-99 0.01962701496175612 100-101 0.2438812130792084 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5190797E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.984073865896953 #Duplication Level Percentage of deduplicated Percentage of total 1 73.866507314453 13.284207237589401 2 10.7194660776728 3.8555933948768857 3 3.7018321391653335 1.9972206788970204 4 2.1547026649738448 1.5500132754373854 5 1.6093071618608508 1.4470949435911258 6 0.8623766549001405 0.9305427277169549 7 0.6024679191224175 0.7584379291531549 8 0.47138401470082175 0.6781923951666106 9 0.49727100905506566 0.8048662702393871 >10 3.950516428819855 14.720842038357079 >50 0.6736619637264897 8.621468304495583 >100 0.766506640467975 30.826434773034624 >500 0.09336571089765151 11.264125089059005 >1k 0.030032867863278086 8.419900969714877 >5k 4.913159153553432E-4 0.574973544504491 >10k+ 1.1011640511684337E-4 0.26608642816634126 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTGTGTTTGAGGGAGGTAGAATTCCACGTGTAGCAGTGAAATGCGTAG 16374 0.10778894616260094 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.053269291927211E-4 0.0 0.0 2 1.053269291927211E-4 0.0 0.0 3 1.1190986226726616E-4 0.0 0.0 4 1.1190986226726616E-4 0.0 0.0 5 1.1190986226726616E-4 0.0 6.582933074545069E-6 6 1.3165866149090135E-4 0.0 6.582933074545069E-6 7 1.3824159456544643E-4 0.0 6.582933074545069E-6 8 1.5799039378908163E-4 0.0 1.3165866149090137E-5 9 1.645733268636267E-4 0.0 1.3165866149090137E-5 10-11 1.90905059161807E-4 0.0 1.6457332686362673E-5 12-13 2.600258564445302E-4 0.0 1.9748799223635204E-5 14-15 3.1927225411543584E-4 0.0 2.9623198835452807E-5 16-17 3.9826745100997665E-4 0.0 3.949759844727041E-5 18-19 4.739711813672449E-4 0.0 4.937199805908801E-5 20-21 5.924639767090562E-4 0.0 5.266346459636055E-5 22-23 7.339970378117751E-4 0.0 5.266346459636055E-5 24-25 8.985703646754019E-4 0.0 5.266346459636055E-5 26-27 0.001109224223060844 0.0 5.595493113363308E-5 28-29 0.0013527927468190114 0.0 5.924639767090561E-5 30-31 0.0016523162017108121 0.0 5.924639767090561E-5 32-33 0.0020242519204226083 0.0 6.253786420817815E-5 34-35 0.0025212633675507612 0.0 6.912079728272322E-5 36-37 0.003100561478110727 0.0 7.899519689454082E-5 38-39 0.0038839305139815906 0.0 8.228666343181336E-5 40-41 0.004914159540147893 0.0 8.55781299690859E-5 42-43 0.006151750958162366 0.0 8.55781299690859E-5 44-45 0.007823815959096814 0.0 8.55781299690859E-5 46-47 0.009890856944503965 0.0 9.216106304363096E-5 48-49 0.012487824042411996 0.0 9.874399611817602E-5 50-51 0.015733210048162714 0.0 1.053269291927211E-4 52-53 0.020071362944287914 0.0 1.053269291927211E-4 54-55 0.025610901126517586 0.0 1.1190986226726616E-4 56-57 0.03252627232132718 0.0 1.1849279534181123E-4 58-59 0.041228909845875766 0.0 1.250757284163563E-4 60-61 0.052857661122059627 3.2914665372725343E-6 1.250757284163563E-4 62-63 0.06756722507713059 6.582933074545069E-6 1.2836719495362881E-4 64-65 0.08605539261699041 6.582933074545069E-6 1.3165866149090135E-4 66-67 0.10951696609466903 6.582933074545069E-6 1.4482452763999148E-4 68-69 0.13982478996987452 6.582933074545069E-6 1.5140746071453656E-4 70-71 0.17735409142785596 6.582933074545069E-6 1.5140746071453656E-4 72-73 0.22374402080417505 6.582933074545069E-6 1.7773919301271687E-4 74-75 0.28246049236257975 6.582933074545069E-6 2.0077945877362458E-4 76-77 0.3569365057014454 6.582933074545069E-6 2.139453249227147E-4 78-79 0.44785339439398736 6.582933074545069E-6 2.205282579972598E-4 80-81 0.5594900649386599 1.3165866149090137E-5 2.3369412414634994E-4 82-83 0.6960464286370228 1.3165866149090137E-5 2.4356852375816753E-4 84-85 0.8612583000088804 1.3165866149090137E-5 2.4685999029544004E-4 86-87 1.0585685530522198 1.3165866149090137E-5 2.7319172259362035E-4 88-89 1.292713608114176 1.3165866149090137E-5 2.89649055279983E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 144290 0.0 67.878075 1 TTTGTGT 155695 0.0 63.053757 2 TAACTTT 15775 0.0 60.744255 1 ACTTTGT 39480 0.0 58.445396 1 AACTTTG 16655 0.0 57.746273 2 TTGTGTT 180610 0.0 55.93589 3 TGTGTTT 190190 0.0 53.37685 4 GTGTTTG 219430 0.0 46.2246 5 TTGAGTC 23265 0.0 37.215836 4 TTGAGGG 32460 0.0 33.51905 9 TTTGAGG 62690 0.0 33.49982 8 TTTGAGT 71160 0.0 32.82627 3 GTTTGAG 173270 0.0 32.27087 7 TGTTTGA 314080 0.0 32.255226 6 TCCGATC 1505 0.0 30.911152 7 TTGAGGC 10625 0.0 30.78335 9 TTGATCT 7980 0.0 30.384659 4 TGAGTCC 18135 0.0 29.919497 5 GTTTGAC 60145 0.0 29.147774 7 TTGAGTG 33170 0.0 28.577703 4 >>END_MODULE