FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l02n01_3522.34100000035b7d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l02n01_3522.34100000035b7d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15190797
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGGGAGGTAGAATTCCACGTGTAGCAGTGAAATGCGTAG163740.10778894616260094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1442900.067.8780751
TTTGTGT1556950.063.0537572
TAACTTT157750.060.7442551
ACTTTGT394800.058.4453961
AACTTTG166550.057.7462732
TTGTGTT1806100.055.935893
TGTGTTT1901900.053.376854
GTGTTTG2194300.046.22465
TTGAGTC232650.037.2158364
TTGAGGG324600.033.519059
TTTGAGG626900.033.499828
TTTGAGT711600.032.826273
GTTTGAG1732700.032.270877
TGTTTGA3140800.032.2552266
TCCGATC15050.030.9111527
TTGAGGC106250.030.783359
TTGATCT79800.030.3846594
TGAGTCC181350.029.9194975
GTTTGAC601450.029.1477747
TTGAGTG331700.028.5777034
Produced by FastQC (version 0.11.2)