FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l01n02_7637.34200000035bca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l01n02_7637.34200000035bca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16049264
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA324800.2023768815816102No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242370.15101627090189307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA18550.065.4051794-95
CTTTGTG1670350.059.78181
TTTGTGT1831950.054.5199282
ACTTTGT549750.052.071281
TTGTGTT1970200.051.825773
ACATCCG19650.051.4531894-95
CGTAGTA19400.048.6418294-95
TGTGTTT2126400.048.2656944
GTGTTTG2310400.044.3763355
TAACTTT287750.043.9503861
AACTTTG294500.043.0876542
CTGCGCA25200.037.44647694-95
TTTGAGG768950.031.3571878
TTGAGGG426500.030.5848399
GTTTGAG1856450.030.451947
TGTTTGA3377200.030.3301986
CGACCTA15652.7894184E-630.14860394-95
GTATAGA16704.6252517E-628.2530394-95
GTTTGAC676100.027.6792187
TTTGAGT653400.027.4318563
Produced by FastQC (version 0.11.2)