FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l01n02_6667.34200000035d92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l01n02_6667.34200000035d92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16679731
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA16000.0197.835394-95
GCGTCGG10750.0100.2391694-95
CTGCCTA16150.091.7436694-95
CTTTGTG1720000.072.457431
TTTGTGT1861150.066.890112
AGAGCGG20850.064.6025894-95
TTGTGTT2046500.062.152653
TGTGTTT2173250.058.744394
ACATCCG16400.057.4923594-95
GTTCTCG13700.054.0751894-95
GTGTTTG2411850.052.939135
ATCGGAG34150.051.27533794-95
CCGCTAG10652.417437E-950.59018794-95
ACTTTGT371600.047.3689731
AACTTTG210250.047.2419362
TAACTTT211600.046.9506651
GAGATCA17600.045.9192294-95
CCTAGCG6107.714936E-444.16274694-95
GGCCTAG6309.043026E-442.76075494-95
CTCGCTG16355.638867E-1141.1915594-95
Produced by FastQC (version 0.11.2)