FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l01n02_6628.34200000035d5f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l01n02_6628.34200000035d5f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16032571
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGGGGTCCATCCCGGATTACCAATCTCAGATAAACTCCG164720.10274085173238902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA7900.0159.5826794-95
GTTCTCG8900.0119.2859294-95
GACCTAG6850.0106.5519394-95
ACATCCG15300.073.7253394-95
GAGCGTA22800.069.8450594-95
CTTTGTG1838650.064.2228241
TTTGTGT1967050.060.0012172
TAACTTT228050.059.6106031
AACTTTG235550.057.9440462
ACTTTGT537750.055.373091
TTGTGTT2224050.054.5177843
ATCGTCA12553.6379788E-1252.8707594-95
TCTCGCG8953.9450242E-851.89603894-95
TGTGTTT2375350.051.3152474
GCGTCGG9255.11518E-850.21292594-95
CGTCGTA11053.8235157E-948.03822394-95
AGCACTA7253.5240882E-545.76054894-95
TATTACA7353.821052E-545.13795594-95
GTGTTTG2754250.044.2746125
CAGTCTA12259.515134E-943.33243694-95
Produced by FastQC (version 0.11.2)