FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l01n02_6617.34200000035d28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l01n02_6617.34200000035d28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16759244
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGGGGTCCATCCCGGATTACCAATCTCAGATAAACTCCG247820.14787063187337088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTCG10200.0171.2290394-95
AGATCGA7550.0160.1510594-95
CTAGTTA12600.0127.9513694-95
ACATCCG9950.0121.52164594-95
CGTCGTA10950.092.0198294-95
ACATCCA40950.078.739394-95
TCCCTCG7359.094947E-1173.1150794-95
TCCTAGG9700.069.2520394-95
CACTGTA8907.2759576E-1267.9292394-95
TCTCGCG8957.2759576E-1267.54973694-95
CTTTGTG1493800.065.2070541
GCGTCGG5254.8469847E-663.9756894-95
GTAGTCA26250.063.9756894-95
TTTGTGT1609700.060.490192
AACTTTG168150.057.7994042
ACTTTGT434150.057.229571
CAGTCTA9458.56744E-1056.86726894-95
TAACTTT170700.056.836491
TTGTGTT1811750.054.9706153
CGTAGTA14750.054.6504194-95
Produced by FastQC (version 0.11.2)