FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l01n02_6471.34200000035cd6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l01n02_6471.34200000035cd6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13389190
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCCG7500.0259.889794-95
GTTCTCG4650.0216.8156694-95
AGATCGA5250.0179.2342894-95
TATATCG3553.5561243E-9113.599294-95
TCTCGCG6900.0107.15092594-95
ACATCCA19300.0100.99341694-95
CTGCGTA4201.1437805E-896.0183794-95
GACCTAG10050.086.94294-95
ATTCTCG3152.9672061E-585.3496694-95
AGAGTTA11900.084.7220994-95
GTGCTCA4151.1949396E-680.9793494-95
AGCACTA5851.2369128E-980.4256494-95
GCGTCGG3555.3676726E-575.73279694-95
CTCTCGG7157.094059E-1175.2031994-95
CATTCGA5709.5116775E-870.7503894-95
ATCGGAC3858.022969E-569.83153594-95
CGTTAGG3959.109182E-568.0636594-95
CTAGTTA22850.067.65407694-95
CGCCCGA3200.003375466463.0120594-95
CTTTGTG1291350.062.8453031
Produced by FastQC (version 0.11.2)