FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l01n02_6271.34200000035c93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l01n02_6271.34200000035c93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16340497
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTCG13700.068.278994-95
ACATCCG21600.064.9597994-95
CTTTGTG1388050.062.8605041
AGATCGA14400.060.31980594-95
TTTGTGT1495850.058.2660182
TCTCGCG12750.057.6449994-95
ACTTTGT411550.056.6141971
TTGTGTT1649700.054.3162733
TGTGTTT1789400.050.3988654
TAACTTT173950.048.5731281
AACTTTG181000.046.760622
GTGTTTG2003650.044.9426965
TCCTAGA12451.0324584E-842.93383494-95
GCGTCGG15904.5474735E-1142.02250394-95
CTTATAG10858.391189E-636.94882294-95
CCGTACG13007.012568E-735.97772694-95
TTGAGTC210000.034.6649254
TCCCTCG10052.3153747E-433.24168494-95
CGTCGTA27050.032.111194-95
TTTGAGG592700.031.7906288
Produced by FastQC (version 0.11.2)