FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l01n02_6205.34200000035c50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l01n02_6205.34200000035c50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15786322
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTT249130.15781383402669727No Hit
CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC173810.11010164368875791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA7500.0293.5910394-95
TATGCTA11600.092.0348194-95
CATGTAA7801.6552804E-1068.4361494-95
CTATATG8252.728484E-1064.7032694-95
ATCGGAC10550.063.24667494-95
GAGCGTA19350.058.62165594-95
ACTAACG11056.002665E-1154.34634894-95
ACTTTGT414600.053.6892551
ATATTAG16200.053.5449494-95
CTTTGTG1019300.052.1748621
GATCACA7052.8900273E-547.32286594-95
CGTAGTA12702.3646862E-1047.28560394-95
TTTGTGT1133500.046.980832
AATTTAG21850.045.806894-95
GTTTATA10401.2308192E-744.91121794-95
TTGTGTT1221500.044.8059923
ATCGGAG22700.044.09156894-95
TCCGAGA7704.8651185E-543.32807594-95
CGTCGTA16105.2750693E-1141.4442594-95
TGTGTTT1337300.041.1169364
Produced by FastQC (version 0.11.2)