FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l01n02_6019.34200000035bf1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l01n02_6019.34200000035bf1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15403891
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA11500.0133.2052994-95
GAGCGTA26250.071.0428294-95
CTTTGTG1453250.060.6301161
TTTGTGT1570150.056.0757332
ACTTTGT470100.054.4396821
CAACCTA23350.051.34251894-95
TTGTGTT1771850.050.8843463
TAACTTT239900.046.897261
TGTGTTT1940150.046.765384
CGTAGTA10009.172982E-846.62185794-95
CGACGTA5756.09134E-446.3322894-95
AACTTTG252600.044.7141532
ATTAGCG7654.7326314E-543.53114794-95
CTGTGTA13101.6640115E-840.67337494-95
CTGCGTA8207.127718E-540.61137494-95
TCCTAGA11803.366331E-739.5100594-95
GTGTTTG2316800.039.206175
AGAGCGG30650.039.1141294-95
GAGATCA14554.197682E-836.62001494-95
ATCGGCA16402.9522198E-936.5502494-95
Produced by FastQC (version 0.11.2)