FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l01n02_3522.34200000035b7a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l01n02_3522.34200000035b7a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14950464
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGGGAGGTAGAATTCCACGTGTAGCAGTGAAATGCGTAG168940.11299983732946349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA6250.0213.703494-95
GTTCTCG8900.097.547294-95
ACATCCG12750.083.8052694-95
TCCCTCG5507.7658115E-872.8534394-95
CTTTGTG1539100.067.002341
CGTCGTA9551.4551915E-1162.93621494-95
TTTGTGT1662650.062.030482
TAACTTT156300.059.0348051
GTTTAGA19450.058.37014894-95
ACTTTGT408300.058.196221
GAGACAA14100.056.8360194-95
AACTTTG163950.056.5584262
AGCACTA4802.4657263E-455.65192894-95
TTGTGTT1899350.055.5995563
CGCCCGA7305.5041164E-754.88957694-95
TGTGTTT1986000.053.4279064
AGAGCGA15350.052.20767294-95
GCGACTA6802.3227398E-549.1046494-95
GCGTCGG8551.6371614E-646.86478494-95
TTATATA5705.7576597E-446.8647894-95
Produced by FastQC (version 0.11.2)