FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l01n01_7637.34100000035bcd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l01n01_7637.34100000035bcd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16049264
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331000.20623998707978136No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA244720.15248051250200634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1591550.061.2960321
TTTGTGT1759900.055.5805742
TTGTGTT1888400.052.930913
ACTTTGT524400.051.475281
TGTGTTT2043900.049.0272834
GTGTTTG2192500.045.6875575
TAACTTT295450.042.414041
AACTTTG299900.042.1863562
TTTGAGG736300.031.8755878
GTTTGAG1773750.031.3908527
TGTTTGA3216750.031.1681566
TTGAGGG405450.030.7462379
GTTTGAC643850.027.8924167
TTTGAGT634400.027.6045043
TTGAGTC242150.025.4230164
GTTTGAT911700.025.170712
TTTGACG195550.024.4944618
TTGAGTG275300.023.9515864
TTTGAGC460150.023.5011338
TTGAGGC127450.023.3168059
Produced by FastQC (version 0.11.2)