FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l01n01_6667.34100000035d95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l01n01_6667.34100000035d95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16679731
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1621550.073.4102251
TTTGTGT1760600.067.801612
TTGTGTT1943650.062.860233
TGTGTTT2067350.059.3429034
GTGTTTG2266600.054.11535
ACTTTGT364650.048.6490361
TAACTTT208350.047.699271
AACTTTG211550.047.3880582
TTTGAGG614700.039.758348
GTTTGAG1694400.038.505127
TTGAGGG329200.038.273139
TGTTTGA3329100.036.8441166
TTGAGGC110600.036.1418049
TTTGAGC411800.034.8386238
TTTGAGT643500.032.6841473
TTTGAGA234850.031.029218
GTTTGAT960800.030.0564527
TTGAGGT187900.029.8384329
TTTGATG267900.029.7530088
GTTTGAC739250.029.7332617