FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l01n01_6628.34100000035d52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l01n01_6628.34100000035d52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16032571
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1731800.064.886131
TTTGTGT1850700.060.8238032
TAACTTT226100.059.7090151
AACTTTG230550.059.0038832
ACTTTGT519650.056.345341
TTGTGTT2106350.055.0239223
TGTGTTT2253850.051.7481844
GTGTTTG2607150.044.7770965
TTTGAGG812100.033.5367628
TTGAGTC256750.032.778414
GTTTGAG2034400.032.0554127
TTGAGGG454000.031.8507639
TGTTTGA3665600.031.8074676
GTTTGAA218050.031.7277972
TCTGTGT22750.031.581092
TTTGAGT789450.030.9325543
TTGAGGC148300.030.218769
TGAGTCT35000.029.5688345
TTGATTC104950.028.9660284
GTTTGAC749750.028.455257