FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l01n01_6617.34100000035d2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l01n01_6617.34100000035d2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16759244
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGGGGTCCATCCCGGATTACCAATCTCAGATAAACTCCG238120.14208278129968155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1413700.066.0199361
TTTGTGT1527350.061.2371832
AACTTTG170450.059.8002742
TAACTTT170850.059.4325561
ACTTTGT423600.056.4986951
TTGTGTT1728350.055.4110573
TGTGTTT1826750.052.6867374
GTGTTTG1967450.048.9175875
TTTGAGG629900.034.3897978
GTTTGAG1664850.033.434017
TGTTTGA2883600.033.3924186
TTGAGGG336450.033.045869
TTTGAGC447650.031.7302658
TTGAGGC110200.031.6633199
GTCCTCG19450.028.8013678
TTTGATG288400.027.7038158
TTTGACC140050.027.4569728
TTTGAGT637050.027.3434753
GTTTGAC548250.027.102947
TTGAGCC262800.027.0786489
Produced by FastQC (version 0.11.2)