FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l01n01_6471.34100000035cd9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l01n01_6471.34100000035cd9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13389190
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1226250.063.709581
AACTTTG156200.060.4886472
TTTGTGT1304650.060.014852
TAACTTT158550.059.3633271
ACTTTGT387850.058.6765981
TTGTGTT1463650.054.8776323
TGTGTTT1560700.051.702874
GTGTTTG1740950.046.351285
TTTGAGG548050.033.5315178
TTGAGGG264750.032.8323979
TTTGACC123850.032.6966488
TTGAGGC93700.032.4257939
GTTTGAG1419950.032.3365367
TGTTTGA2499200.032.2656446
GTTTGAA154300.031.4492242
TTGACCC62650.030.7648779
TGAGTCT31000.029.8622935
TTTGAGC348600.029.1023048
GAGGTCT17300.028.5389166
TTTGAGT524250.028.5356643
Produced by FastQC (version 0.11.2)