FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l01n01_6271.34100000035c96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l01n01_6271.34100000035c96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16340497
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1294400.063.860161
TTTGTGT1397100.059.250982
ACTTTGT389950.055.9695741
TTGTGTT1543700.055.277743
TGTGTTT1683600.051.0404934
TAACTTT171400.050.5057371
AACTTTG183750.047.186712
GTGTTTG1853150.046.401855
TTGAGTC197850.033.0435524
TGTTTGA2827400.032.494631
GTTTGAG1559550.032.381472
TTTGAGG574250.032.264818
TTTGAGT601700.031.5205153
TTGAGGG275250.030.5092879
TTTGAGC442850.025.850863
GTTTGAC538650.025.778097
TTGAGGC102300.025.5686329
TTGAGGT199050.025.112959
TGAGTCT47200.025.0431485
GTTTGAA271250.024.5791592