FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l01n01_6205.34100000035c53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l01n01_6205.34100000035c53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15786322
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTT232470.1472603941564096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT391050.053.4699631
CTTTGTG953950.052.7550541
TTTGTGT1058700.047.6496352
TTGTGTT1140600.045.5044363
TGTGTTT1255450.041.576584
GTGTTTG1420200.036.816835
GTTTGAG1131100.028.2370532
TGTTTGA1938300.027.6883531
TTTGAGG544250.026.918348
TTGAGGG391100.025.175139
TTTGAGT379250.024.9876923
TTGAGTC122650.024.3587194
AACTTTG389200.023.8010922
TAACTTT385600.023.7059671
GTTTGAC389650.022.6006037
GTTTGAA126750.022.221712
TGAGGGG320600.021.5303785
TTTGAGA163500.020.6586993
TTTGATG134950.020.122138
GTTTGAT565800.020.0721192
Produced by FastQC (version 0.11.2)